Corynebacterium sputi (DSM 45148, CCUG 55795, CIP 109925)

Name: Corynebacterium sputi (DSM 45148, CCUG 55795, CIP 109925)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 3228

Type strain

Culture col. no. DSM 45148 CCUG 55795 CIP 109925 JCM 17321 IMMIB L-999

NCBI tax ID(s) 1111729 489915

Special Collections DSMZ CCUG JCM CIP Literature strains

History <- A. F. Yassin, Inst. Med. Mikrobiol. Immun. Univ. Bonn; IMMIB L-999 CCUG 55795 <-- A. F. Yassin IMMIB L-999. CIP <- 2011, CCUG <- 2008, A.F. Yassin, Bonn Univ. Bonn, Germany: strain IMMIB L-999

Links

Corynebacterium sputi DSM 45148 is a facultative anaerobe, mesophilic, Gram-positive prokaryote that was isolated from sputum of a patient with pneumonia.

Gram-positive rod-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 360269

CCUG 55795,DSM 45148,JCM 17321,CIP 109925

@ref 20215

Last LPSN update 2025-12-16 09:47:30

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Corynebacteriaceae

Genus Corynebacterium

Species Corynebacterium sputi

Full scientific name Corynebacterium sputi Yassin and Siering 2008

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
31188	positive	rod-shaped
123277	positive	rod-shaped
125438			90.5
125438	positive		90.279

Pigmentation

31188

Productionyes

Multimedia

@ref:	15415
multimedia content:	DSM_45148.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45148.jpg
caption:	Medium 535a 37°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15415	TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
34075	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123277	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15415	positive	growth	37	mesophilic
31188	positive	growth	37	mesophilic
31188	positive	optimum	37	mesophilic
34075	positive	growth	37	mesophilic
60736	positive	growth	30	mesophilic
67770	positive	growth	37	mesophilic

Culture pH

@ref	Ability	Type	PH
31188	positive	optimum	7
31188	positive	growth	7

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31188	facultative anaerobe
60736	microaerophile
125439	obligate aerobe	93.2

Spore formation

31188

Spore formationno

Observation

31188

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31188	17234 ChEBI	glucose	+	carbon source
123277	17632 ChEBI	nitrate	-	reduction
123277	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123277	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	-	3.4.21.4	from API zym
31188	urease	+	3.5.1.5
123277	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123277	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Inflammation	-
#Infection	#Patient	-
#Host Body-Site	#Oral cavity and airways	#Airways
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
15415	sputum of a patient with pneumonia		Bonn	Germany	DEU	Europe
60736	Human sputum,pneumonia	1996-02-01	Bonn	Germany	DEU	Europe
67770	Sputum of a patient with pneumonia
123277	Human, Sputum pneumonia		Bonn	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence AM930556 (>99% sequence identity) for Corynebacterium sputi subclade from Microbeatlas

Aquatic Samples	643
Soil Samples	670
Animal Samples	3569
Plant Samples	288
Total Samples	5170

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
15415	yes, in single cases	1	Risk group (German classification) According to TRBA 466
123277		2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM42786v1 assembly for Corynebacterium sputi DSM 45148	scaffold	GCA_000427865	1111729.3	2513237121	1111729	70.69

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15415	Corynebacterium sputi partial 16S rRNA gene, type strain IMMIB L-999T	AM930556	1495		1111729

GC content

@ref	GC-content (mol%)	Method
67770	61.5	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Corynebacterium sputi DSM 45148
NCBI: GCA_000427865

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	93.20	no
125439	gram_stain	BacteriaNetⓘ	positive	78.40	no
125439	motility	BacteriaNetⓘ	no	61.30	no
125439	spore_formation	BacteriaNetⓘ	no	89.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Corynebacterium sputi DSM 45148
PATRIC: 1111729.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.28	yes
125438	anaerobic	anaerobicⓘ	no	97.69	no
125438	spore-forming	spore-formingⓘ	no	75.53	no
125438	aerobic	aerobicⓘ	yes	66.01	yes
125438	thermophilic	thermophileⓘ	no	94.10	no
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Influence of Endogenous Bacteria on Behavioral Responses in Leptocybe invasa: An Analysis of mVOCs.	Zhou L, Hu P, Xie J, Li J, Guo C, Yang Z.	Insects	10.3390/insects15060455	2024
	Phylogenomic Reappraisal of Fatty Acid Biosynthesis, Mycolic Acid Biosynthesis and Clinical Relevance Among Members of the Genus Corynebacterium.	Dover LG, Thompson AR, Sutcliffe IC, Sangal V.	Front Microbiol	10.3389/fmicb.2021.802532	2021
Phylogeny	Role of Williamsia and Segniliparus in human infections with the approach taxonomy, cultivation, and identification methods.	Fatahi-Bafghi M.	Ann Clin Microbiol Antimicrob	10.1186/s12941-021-00416-z	2021
Phylogeny	Corynebacterium sputi sp. nov., isolated from the sputum of a patient with pneumonia.	Yassin AF, Siering C	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/000414-0	2008

References

#15415	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45148
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27515	IJSEM 2876 2008 ( DOI 10.1099/ijs.0.2008/000414-0 , PubMed 19060075 )
#31188	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27515
#34075	; Curators of the CIP;
#60736	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55795
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123277	Collection of Institut Pasteur ; Curators of the CIP; CIP 109925
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Corynebacterium sputi (DSM 45148, CCUG 55795, CIP 109925)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000427865

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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