Name: Corynebacterium sputi DSM 45148
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3228

Type strain:

Species: Corynebacterium sputi

Culture col. no.: DSM 45148, CCUG 55795, CIP 109925, JCM 17321, IMMIB L-999

Strain history: CIP <- 2011, CCUG <- 2008, A.F. Yassin, Bonn Univ. Bonn, Germany: strain IMMIB L-999

NCBI tax ID(s): 1111729 (strain), 489915 (species)

SI-ID 360269

CCUG 55795, DSM 45148, JCM 17321, CIP 109925

Section

Corynebacterium sputi DSM 45148 is a facultative anaerobe, mesophilic, Gram-positive human pathogen that was isolated from sputum of a patient with pneumonia.

Gram-positive
rod-shaped
facultative anaerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium sputi**
	Full Scientific Name (LPSN)	Corynebacterium sputi Yassin and Siering 2008

Information on morphological and physiological properties Morphology

[Ref.: #31188]	Gram stain	positive
[Ref.: #123277]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31188]	Cell shape	rod-shaped
[Ref.: #123277]	Cell shape	rod-shaped

[Ref.: #31188]	Motility	no
[Ref.: #123277]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.617

[Ref.: #31188]

Pigment production

yes

Multimedia content

[Ref.: #15415]

Caption:

Medium 535a 37°C

[Ref.: #15415]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15415]

Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)

[Ref.: #15415]

Culture medium growth

[Ref.: #15415]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15415]

Culture medium composition

expand / minimize

[Ref.: #34075]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #34075]

Culture medium growth

[Ref.: #34075]

Culture medium composition

expand / minimize

[Ref.: #123277]

Culture medium

CIP Medium 6

[Ref.: #123277]

Culture medium growth

[Ref.: #123277]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15415]	growth	37 °C
[Ref.: #31188]	growth	37 °C
[Ref.: #31188]	optimum	37 °C
[Ref.: #34075]	growth	37 °C
[Ref.: #60736]	growth	30 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #15415]	Temperature range	mesophilic
[Ref.: #31188]	Temperature range	mesophilic
[Ref.: #34075]	Temperature range	mesophilic
[Ref.: #60736]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31188]			optimum	7
[Ref.: #31188]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31188]	Oxygen tolerance	facultative anaerobe
[Ref.: #60736]	Oxygen tolerance	microaerophile

[Ref.: #31188]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.982

[Ref.: #31188]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31188]	glucose ChEBI	+	carbon source
[Ref.: #123277]	nitrate ChEBI	-	reduction
[Ref.: #123277]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123277]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #31188]	urease	+	3.5.1.5
[Ref.: #123277]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123277]	12223	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15415]	Sample type/isolated from	sputum of a patient with pneumonia
[Ref.: #15415]	Geographic location (country and/or sea, region)	Bonn
[Ref.: #15415]	Country	Germany
[Ref.: #15415]	Country ISO 3 Code	DEU
[Ref.: #15415]	Continent	Europe

[Ref.: #60736]	Sample type/isolated from	Human sputum,pneumonia
[Ref.: #60736]	Sampling date	1996-02-01
[Ref.: #60736]	Geographic location (country and/or sea, region)	Bonn
[Ref.: #60736]	Country	Germany
[Ref.: #60736]	Country ISO 3 Code	DEU
[Ref.: #60736]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Sputum of a patient with pneumonia

[Ref.: #123277]	Sample type/isolated from	Human, Sputum pneumonia
[Ref.: #123277]	Geographic location (country and/or sea, region)	Bonn
[Ref.: #123277]	Country	Germany
[Ref.: #123277]	Country ISO 3 Code	DEU
[Ref.: #123277]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Inflammation	-
#Infection	#Patient	-
#Host Body-Site	#Oral cavity and airways	#Airways
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM930556 (>99% sequence identity) for Corynebacterium sputi subclade from Microbeatlas

Aquatic Samples	643
Soil Samples	670
Animal Samples	3569
Plant Samples	288
Total Samples	5170

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15415]	Pathogenicity (human)	yes, in single cases
[Ref.: #15415]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123277]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15415]	Corynebacterium sputi partial 16S rRNA gene, type strain IMMIB L-999T	AM930556	1495	ENA	1111729 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Corynebacterium sputi DSM 45148	GCA_000427865	scaffold	GenBank	1111729 tax ID
[Ref.: #66792]	Corynebacterium sputi DSM 45148	1111729.3	wgs	PATRIC	1111729 tax ID	*
[Ref.: #66792]	Corynebacterium sputi DSM 45148	2513237121	draft	JGI IMG/M	1111729 tax ID	*

[Ref.: #67770]

GC-content

61.5 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Corynebacterium sputi DSM 45148 DSM 45148 NCBI: GCA_000427865.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium sputi DSM 45148 PATRIC: 1111729.3
[Ref.: #69480]	motile	no	94.864	yes
[Ref.: #69480]	flagellated	no	97.231	yes
[Ref.: #69480]	gram-positive	yes	92.042	no
[Ref.: #69480]	anaerobic	no	98.833	yes
[Ref.: #69480]	aerobic	yes	66.888	yes
[Ref.: #69480]	halophile	yes	61.453	no
[Ref.: #69480]	spore-forming	no	89.344	no
[Ref.: #69480]	thermophile	no	98.441	yes
[Ref.: #69480]	glucose-util	yes	88.254	yes
[Ref.: #69480]	glucose-ferment	no	65.141	no

Availability in culture collections External links

[Ref.: #15415]

Culture collection no.

DSM 45148, CCUG 55795, CIP 109925, JCM 17321, IMMIB L-999

[Ref.: #72742]

SI-ID 360269

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Corynebacterium sputi sp. nov., isolated from the sputum of a patient with pneumonia.	Yassin AF, Siering C	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/000414-0	2008	*

References

#15415

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45148

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31188

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27515 (see below)

#34075 ; Curators of the CIP;
#60736 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55795

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72742

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID360269.1 )

#123277 Collection of Institut Pasteur ; Curators of the CIP; CIP 109925

#27515

IJSEM 2876 2008 ( DOI 10.1099/ijs.0.2008/000414-0 , PubMed 19060075 )

* These data were automatically processed and therefore are not curated

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Corynebacterium sputi DSM 45148 DSM 45148

Corynebacterium sputi DSM 45148

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