Corynebacterium singulare (DSM 44357, CCUG 37330, CIP 105491)

Name: Corynebacterium singulare (DSM 44357, CCUG 37330, CIP 105491)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 3182

Type strain

Culture col. no. DSM 44357 CCUG 37330 CIP 105491 IBS B52218 IFO 16162 4 more

NCBI tax ID(s) 161899

Special Collections DSMZ CCUG JCM CIP Wink compendium Literature strains

Links

Corynebacterium singulare DSM 44357 is a facultative anaerobe, mesophilic, Gram-positive prokaryote that was isolated from human semen.

Gram-positive rod-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 264413

LMG 19056,CCUG 37330,CIP 105491,DSM 44357,IFO 16162,CCM 4729,JCM 10385,NBRC 16162

@ref 20215

Last LPSN update 2025-12-16 09:47:29

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Corynebacteriaceae

Genus Corynebacterium

Species Corynebacterium singulare

Full scientific name Corynebacterium singulare Riegel et al. 1997

BacDive ID	Other strains from **Corynebacterium singulare** (1)	Type strain
151932	C. singulare CCUG 45243

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
23180	positive	rod-shaped
122632	positive	rod-shaped
125438			95.5
125438	positive		91.716

Colony morphology

@ref	Type of hemolysis	Colony size	Colony color	Colony shape	Incubation period	Medium used
20050			Ivory (1014)		10-14 days	ISP 2
20050			Ivory (1014)		10-14 days	ISP 3
20050			Ivory (1014)		10-14 days	ISP 4
20050			Ivory (1014)		10-14 days	ISP 5
20050			Ivory (1014)		10-14 days	ISP 6
20050			Ivory (1014)		10-14 days	ISP 7
23180	gamma	1-2 mm	grayish	circular	1 day	sheep blood agar

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20050		ISP 2
20050		ISP 3
20050		ISP 4
20050		ISP 5
20050		ISP 6
20050		ISP 7

Multimedia

@ref:	11701
multimedia content:	DSM_44357.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44357.jpg
caption:	Medium 535a 37°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11701	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
11701	TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
20050	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20050	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20050	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20050	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20050	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20050	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
23180	sheep blood agar
34670	MEDIUM 45 - for Columbia agar with sheep blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
122632	CIP Medium 72	Medium recipe at CIP
122632	CIP Medium 45	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11701	positive	growth	37	mesophilic
20050	positive	optimum	37	mesophilic
23180	positive	growth	37	mesophilic
34670	positive	growth	37	mesophilic
53610	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
122632	positive	growth	25-41
122632	negative	growth	10
122632	negative	growth	15
122632	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
23180	facultative anaerobe
53610	aerobe
122632	facultative anaerobe

Spore formation

@ref	Spore formation	Confidence
20050
23180
125439		93.1

Halophily

@ref	Salt	Growth	Tested relation	Concentration
122632	NaCl	positive	growth	0-10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23180	37054 ChEBI	3-hydroxybutyrate	+	carbon source
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
23180	15570 ChEBI	D-alanine	+	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
23180	15824 ChEBI	D-fructose	+	carbon source
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
23180	8391 ChEBI	D-gluconate	-	carbon source
23180	17634 ChEBI	D-glucose	+	carbon source
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
23180	16024 ChEBI	D-mannose	+	carbon source
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
23180	16988 ChEBI	D-ribose	-	carbon source
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
23180	65327 ChEBI	D-xylose	-	builds acid from
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
23180	4853 ChEBI	esculin	-	hydrolysis
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
122632	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
23180	29806 ChEBI	fumarate	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
23180	5291 ChEBI	gelatin	-	hydrolysis
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
23180	17234 ChEBI	glucose	+	builds acid from
23180	17754 ChEBI	glycerol	+	carbon source
23180	28087 ChEBI	glycogen	-	builds acid from
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
122632	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
23180	29991 ChEBI	L-aspartate	+	carbon source
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
23180	29985 ChEBI	L-glutamate	+	carbon source
23180	15971 ChEBI	L-histidine	+	carbon source
23180	15589 ChEBI	L-malate	+	carbon source
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
23180	17115 ChEBI	L-serine	+	carbon source
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
23180	17895 ChEBI	L-tyrosine	+	carbon source
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
23180	24996 ChEBI	lactate	+	carbon source
23180	17716 ChEBI	lactose	-	builds acid from
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
23180	17306 ChEBI	maltose	+	builds acid from
23180	17306 ChEBI	maltose	+	carbon source
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
23180	29864 ChEBI	mannitol	-	builds acid from
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
23180	506227 ChEBI	N-acetylglucosamine	-	carbon source
23180	17632 ChEBI	nitrate	-	reduction
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
122632	17632 ChEBI	nitrate	-	reduction
122632	17632 ChEBI	nitrate	+	respiration
122632	16301 ChEBI	nitrite	-	reduction
23180	25646 ChEBI	octanoate	+	carbon source
23180	18401 ChEBI	phenylacetate	+	carbon source
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
23180	17272 ChEBI	propionate	+	carbon source
23180	17148 ChEBI	putrescine	+	carbon source
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
23180	33942 ChEBI	ribose	-	builds acid from
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
23180	30031 ChEBI	succinate	+	carbon source
23180	17992 ChEBI	sucrose	+	builds acid from
23180	17992 ChEBI	sucrose	+	carbon source
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
23180	27082 ChEBI	trehalose	-	builds acid from
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
23180	32528 ChEBI	turanose	+	carbon source
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
23180	18186 ChEBI	tyrosine	+	degradation
23180	16199 ChEBI	urea	+	degradation
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
122632	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122632	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
122632	15688 ChEBI	acetoin	+
122632	17234 ChEBI	glucose		+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
122632	alcohol dehydrogenase	-	1.1.1.1
23180	alkaline phosphatase	+	3.1.3.1
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
23180	alpha-glucosidase	-	3.2.1.20
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
122632	amylase	+
23180	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
122632	beta-galactosidase	-	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
23180	beta-glucuronidase	-	3.2.1.31
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
122632	caseinase	-	3.4.21.50
23180	catalase	+	1.11.1.6
122632	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
23180	cytochrome oxidase	-	1.9.3.1
122632	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
122632	gamma-glutamyltransferase	+	2.3.2.2
122632	gelatinase	-
68379	gelatinase	-		from API Coryne
122632	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
122632	lipase	-
68382	lipase (C 14)	-		from API zym
122632	lysine decarboxylase	-	4.1.1.18
23180	N-acetyl-beta-glucosaminidase	-	3.2.1.52
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122632	ornithine decarboxylase	-	4.1.1.17
122632	oxidase	-
122632	phenylalanine ammonia-lyase	-	4.3.1.24
23180	pyrazinamidase	+	3.5.1.B15
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
23180	pyrrolidonyl arylamidase	+	3.4.19.3
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
122632	tryptophan deaminase	-
122632	tween esterase	+
122632	urease	+	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
20050	+	-	-	-	-	-	-	-	-	+	-	not determinedn.d.	+	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20050	not determinedn.d.	-	+	+	-	+	-	-	+	-	+	+	-	-	-	-	-	-	-	-
122632	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
122632	not determinedn.d.	+/-	-	-	-	+/-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
122632	not determinedn.d.	+	+	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	-	-	+	+	+	-	-	+	-	-	+	-	-	+	+	-	+	+	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Urogenital tract	#Semen
#Host	#Human	-
#Host	#Human	#Male

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11701	human semen	Strasbourg	France	FRA	Europe
53610	Human semen	Strasbourg	France	FRA	Europe
67770	Human semen specimen	Strasbourg	France	FRA	Europe
122632	Human, Semen	Strasbourg	France	FRA	Europe

Taxonmaps

69479

Global distribution of 16S sequence Y10999 (>99% sequence identity) for Corynebacterium from Microbeatlas

Aquatic Samples	1218
Soil Samples	1440
Animal Samples	18979
Plant Samples	422
Total Samples	22059

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
11701	yes, in single cases	1	Risk group (German classification) According to TRBA 466
20050		1	German classification According to TRBA 466
122632		2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM83357v1 assembly for Corynebacterium singulare IBS B52218	complete	GCA_000833575	161899.3	2600254920	161899	99.66

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
23180	Corynebacterium singulare 16S rRNA gene, strain IBS B52218; CCUG 37330	Y10999	1323		161899

GC content

@ref	GC-content (mol%)	Method
11701	62
23180	62.0	high performance liquid chromatography (HPLC)
67770	62	capillary zone electrophoresis (CZE)
67770	60.12	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Corynebacterium singulare IBS B52218
PATRIC: 161899.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	93.10	no
125439	motility	BacteriaNetⓘ	no	74.00	no
125439	gram_stain	BacteriaNetⓘ	positive	78.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	85.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Corynebacterium singulare IBS B52218
PATRIC: 161899.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.72	yes
125438	anaerobic	anaerobicⓘ	no	94.26	no
125438	spore-forming	spore-formingⓘ	no	80.35	yes
125438	aerobic	aerobicⓘ	yes	55.72	no
125438	thermophilic	thermophileⓘ	no	95.50	no
125438	flagellated	motile2+ⓘ	no	95.50	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	A Review on Microbial Species for Forensic Body Fluid Identification in Healthy and Diseased Humans.	Dass M, Singh Y, Ghai M.	Curr Microbiol	10.1007/s00284-023-03413-x	2023
Enzymology	AAC(3)-XI, a new aminoglycoside 3-N-acetyltransferase from Corynebacterium striatum.	Galimand M, Fishovitz J, Lambert T, Barbe V, Zajicek J, Mobashery S, Courvalin P.	Antimicrob Agents Chemother	10.1128/aac.01203-15	2015
	Study of Antibiotic Resistance Potential of Spiders Bacteriota and Comparison With the Antibacterial Effect of Essential Oils.	Kacaniova M, Elizondo-Luevano JH, Ban Z, Babosova M, Porhajasova JI, Kollar J, Kowalczewski PL, Garzoli S.	Microbiologyopen	10.1002/mbo3.70145	2025
Genetics	Exploring antimicrobial resistance determinants in the Neanderthal microbiome.	Sankaranarayanan G, Kodiveri Muthukaliannan G.	Microbiol Spectr	10.1128/spectrum.02662-23	2024
Genetics	Revealing the diversity of commensal corynebacteria from a single human skin site.	Herman R, Meaden S, Rudden M, Cornmell R, Wilkinson HN, Hardman MJ, Wilkinson AJ, Murphy B, Thomas GH.	mSystems	10.1128/msystems.00459-25	2025
	Possible Implications of Bacteriospermia on the Sperm Quality, Oxidative Characteristics, and Seminal Cytokine Network in Normozoospermic Men.	Tvrda E, Lovisek D, Galova E, Schwarzova M, Kovacikova E, Kunova S, Ziarovska J, Kacaniova M.	Int J Mol Sci	10.3390/ijms23158678	2022
Genetics	Protein domain architectures provide a fast, efficient and scalable alternative to sequence-based methods for comparative functional genomics.	Koehorst JJ, Saccenti E, Schaap PJ, Martins Dos Santos VAP, Suarez-Diez M.	F1000Res	10.12688/f1000research.9416.3	2016
	Molecular analysis of human forearm superficial skin bacterial biota.	Gao Z, Tseng CH, Pei Z, Blaser MJ.	Proc Natl Acad Sci U S A	10.1073/pnas.0607077104	2007
Phylogeny	Prevalence of corynebacterial 16S rRNA sequences in patients with bacterial and "nonbacterial" prostatitis.	Tanner MA, Shoskes D, Shahed A, Pace NR.	J Clin Microbiol	10.1128/jcm.37.6.1863-1870.1999	1999
Genetics	Complete Genome Sequence and Annotation of Corynebacterium singulare DSM 44357, Isolated from a Human Semen Specimen.	Merten M, Brinkrolf K, Albersmeier A, Kutter Y, Ruckert C, Tauch A	Genome Announc	10.1128/genomeA.00183-15	2015
Phylogeny	Description of Corynebacterium hiratae sp. nov. isolated from a human tissue bone a novel member of Corynebacterium Genus.	de Oliveira Sant'Anna L, Dos Santos LS, Olivella JGB, da Cruz Mota M, Ramos JN, Baio PVP, da Rocha DJPG, Vieira VV, Almuzara M, Vay C, Barberis C, Castro TLP, Seyffert N, Pacheco LGC, Mattos-Guaraldi AL.	Braz J Microbiol	10.1007/s42770-024-01331-z	2024
Phylogeny	Corynebacterium tapiri sp. nov. and Corynebacterium nasicanis sp. nov., isolated from a tapir and a dog, respectively.	Baumgardt S, Loncaric I, Kampfer P, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000510	2015
Phylogeny	Corynebacterium singulare sp. nov., a new species for urease-positive strains related to Corynebacterium minutissimum.	Riegel P, Ruimy R, Renaud FN, Freney J, Prevost G, Jehl F, Christen R, Monteil H	Int J Syst Bacteriol	10.1099/00207713-47-4-1092	1997

References

#11701	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44357
#20050	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23180	Philippe Riegel, Raymond Ruimy, Francois N. R. Renaud, Jean Freney, Gilles Prevost, Francois Jehl, Richard Christen, Henri Monteil: Corynebacterium singulare sp. nov., a New Species for Urease-Positive Strains Related to Corynebacterium minutissimum. IJSEM 47: 1092 - 1096 1997 ( DOI 10.1099/00207713-47-4-1092 , PubMed 9336912 )
#34670	; Curators of the CIP;
#53610	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37330
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122632	Collection of Institut Pasteur ; Curators of the CIP; CIP 105491
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Corynebacterium singulare (DSM 44357, CCUG 37330, CIP 105491)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API coryne

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000833575

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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