Corynebacterium diphtheriae C7s, C7 s (-) tox- | Type strain | DSM 44123, ATCC 27010, CIP 100721, NCTC 11397, CCUG 37874

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Intellectual property rights:

Caption:

Medium 535a 37°C

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Information on culture and growth conditions Culture and growth conditions

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

ISP 2

Culture medium growth

Culture medium composition

Culture medium

ISP 3

Culture medium growth

Culture medium composition

Culture medium

ISP 4

Culture medium growth

Culture medium composition

Culture medium

ISP 5

Culture medium growth

Culture medium composition

Culture medium

ISP 6

Culture medium growth

Culture medium composition

Culture medium

ISP 7

Culture medium growth

Culture medium composition

[Ref.: #37677]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37677]

Culture medium growth

[Ref.: #37677]

Culture medium composition

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

CIP Medium 3

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

Culture medium

CIP Medium 72

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

	Kind of temperature		Temperature
[Ref.: #11482]		growth	37 °C
[Ref.: #19631]		optimum	37 °C
[Ref.: #37677]		growth	37 °C
[Ref.: #118942]		growth	37 °C
[Ref.: #118942]	no	growth	41 °C

[Ref.: #11482]	Temperature range	mesophilic
[Ref.: #19631]	Temperature range	mesophilic
[Ref.: #37677]	Temperature range	mesophilic
[Ref.: #118942]	Temperature range	mesophilic
[Ref.: #118942]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

Only first 5 entries are displayed. Click here to see all.

Oxygen tolerance

obligate aerobe

[Ref.: #19631]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.99

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #118942]	nitrate ChEBI	+	reduction
[Ref.: #118942]	nitrate ChEBI	+	respiration
[Ref.: #118942]	nitrite ChEBI	-	reduction
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne

	Metabolite production	Metabolite	Production
[Ref.: #118942]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #118942]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118942]	amylase	+
[Ref.: #118942]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #118942]	catalase	+	1.11.1.6
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #118942]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118942]	gelatinase	-
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #118942]	lecithinase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #118942]	oxidase	-
[Ref.: #118942]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118942]	tween esterase	-
[Ref.: #118942]	urease	-	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118942]	47	not determinedn.d.	+	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #19631]	211	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.
[Ref.: #11482]	4307	+	-	-	-	-	-	+	-	-	-	-	-	+	+	-	-	+	-	-	-	+
[Ref.: #11482]	4288	+	-	-	-	-	-	+	-	-	-	-	-	+	+/-	-	-	+	-	-	-	+
[Ref.: #53924]	1293	+	-	-	-	-	-	+	-	-	-	-	-	+	+	-	-	+	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19631]	458	not determinedn.d.	-	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #53924]	1753	-	-	+	+	-	-	-	-	-	-	+	+	-	-	-	+	-	-	-	-
[Ref.: #118942]	6628	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11482]	Geographic location (country and/or sea, region)	New York
[Ref.: #11482]	Country	USA
[Ref.: #11482]	Country ISO 3 Code	USA
[Ref.: #11482]	Continent	North America

[Ref.: #53924]	Geographic location (country and/or sea, region)	New York ?
[Ref.: #53924]	Country	USA
[Ref.: #53924]	Country ISO 3 Code	USA
[Ref.: #53924]	Continent	North America
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence X82059 (>99% sequence identity) for Corynebacterium diphtheriae from Microbeatlas

Aquatic Samples	4794
Soil Samples	3296
Animal Samples	29947
Plant Samples	1078
Total Samples	39115

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11482]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #19631]	Biosafety level	2	Risk group (German classification)
[Ref.: #118942]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Corynebacterium diphtheriae strain ATCC 27010 16S ribosomal RNA gene, partial sequence	GQ118341	1441	ENA	1717 tax ID	*
[Ref.: #20218]	C.diphtheriae 16S ribosomal DNA	X82059	1391	ENA	1717 tax ID	*
[Ref.: #20218]	Corynebacterium diphtheriae 16S rRNA gene, strain NCTC 11397	X84248	1485	ENA	1717 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Corynebacterium diphtheriae strain NCTC11397	1717.769	complete	PATRIC	1717 tax ID	*
[Ref.: #66792]	Corynebacterium diphtheriae bv. gravis DSM 44123	GCA_001913265	contig	GenBank	1720349 tax ID	*
[Ref.: #66792]	Corynebacterium diphtheriae bv. gravis strain DSM 44123	1720349.3	wgs	PATRIC	1720349 tax ID	*
[Ref.: #66792]	Corynebacterium diphtheriae strain NCTC11397	1717.62	complete	PATRIC	1717 tax ID	*
[Ref.: #66792]	Corynebacterium diphtheriae NCTC11397	2660238345	complete	JGI IMG/M	1717 tax ID	*
[Ref.: #66792]	Corynebacterium diphtheriae bv. gravis DSM 44123	2916404855	draft	JGI IMG/M	1720349 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Corynebacterium diphtheriae NCTC11397 NCBI: GCA_001457455.1
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium diphtheriae NCTC11397 NCBI: GCA_001457455.1
[Ref.: #69480]	flagellated	no	97.67	no
[Ref.: #69480]	gram-positive	yes	92.877	no
[Ref.: #69480]	anaerobic	no	97.217	no
[Ref.: #69480]	aerobic	no	84.522	no
[Ref.: #69480]	halophile	yes	66.026	no
[Ref.: #69480]	spore-forming	no	94.119	yes
[Ref.: #69480]	glucose-util	yes	88.43	no
[Ref.: #69480]	motile	no	95.21	no
[Ref.: #69480]	thermophile	no	92.685	yes
[Ref.: #69480]	glucose-ferment	yes	80.049	no

Availability in culture collections External links