Corynebacterium amycolatum S160 | Type strain | DSM 6922, ATCC 49368, NCFB 2768, CCUG 35685, LMG 19043, JCM 7447, CCM 4123, CECT 4163, CGMCC 1.1939, CIP 103452, IFO 15207, KCTC 3432, NBRC 15207, NCIMB 13130

Intellectual property rights:

Caption:

Medium 535a 37°C

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

ISP 2

Culture medium growth

Culture medium composition

Culture medium

ISP 4

Culture medium growth

Culture medium composition

Culture medium

ISP 5

Culture medium growth

Culture medium composition

Culture medium

ISP 6

Culture medium growth

Culture medium composition

Culture medium

ISP 7

Culture medium growth

Culture medium composition

[Ref.: #34280]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #34280]

Culture medium growth

[Ref.: #34280]

Culture medium composition

Culture medium

CIP Medium 3

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

Culture medium

CIP Medium 72

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

	Kind of temperature		Temperature
[Ref.: #2888]		growth	37 °C
[Ref.: #20198]		optimum	28 °C
[Ref.: #34280]		growth	37 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #116349]		growth	30-41 °C
[Ref.: #116349]	negative	growth	10 °C
[Ref.: #116349]	negative	growth	15 °C
[Ref.: #116349]	negative	growth	25 °C
[Ref.: #116349]	negative	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116349]	Oxygen tolerance	obligate aerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %94.8

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #20198]		NaCl		maximum	7.5	%
[Ref.: #116349]		NaCl		growth	0-6	%

[Ref.: #2888]	Murein short key	A31
[Ref.: #2888]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-9(H₂) (MK-8(H₂))

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #20198]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #20198]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne, API 20E
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #116349]	esculin ChEBI	-	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #20198]	fructose ChEBI	+
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne, API 20E
[Ref.: #20198]	glucose ChEBI	+
[Ref.: #116349]	hippurate ChEBI	+	hydrolysis
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne
[Ref.: #20198]	mannitol ChEBI	-
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #20198]	myo-inositol ChEBI	-
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #116349]	nitrate ChEBI	-	reduction
[Ref.: #116349]	nitrate ChEBI	-	respiration
[Ref.: #116349]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #20198]	raffinose ChEBI	-
[Ref.: #20198]	rhamnose ChEBI	-
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #20198]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne, API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne, API 20E
[Ref.: #20198]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #116349]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite	Production
[Ref.: #116349]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #116349]	acetoin ChEBI	+
[Ref.: #116349]	glucose ChEBI		+
[Ref.: #68368]	acetoin ChEBI	+			* from API 20E
[Ref.: #68368]	indole ChEBI			-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116349]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116349]	amylase	+
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #116349]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne, API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #116349]	caseinase	-	3.4.21.50
[Ref.: #116349]	catalase	+	1.11.1.6
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #116349]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116349]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #116349]	gelatinase	-
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20E
[Ref.: #116349]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #116349]	lipase	-
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #116349]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116349]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #116349]	oxidase	-
[Ref.: #116349]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #116349]	protease	-
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116349]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #116349]	tween esterase	-
[Ref.: #116349]	urease	+	3.5.1.5
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne, API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20198]	641	-	-	-	-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #116349]	417	not determinedn.d.	+	+	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #20198]	467	+	-	-	+	-	-	-	-	-	+	-	not determinedn.d.	+	+	+	+	+	+	-	+	not determinedn.d.
[Ref.: #2888]	4434	-	-	-	+	-	-	-	-	-	+	-	-	+	+/-	-	-	+	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20198]	977	not determinedn.d.	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-
[Ref.: #116349]	7543	-	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2888]	Sample type/isolated from	human skin

[Ref.: #52916]	Sample type/isolated from	Human cutaneous
[Ref.: #52916]	Country	United Kingdom
[Ref.: #52916]	Country ISO 3 Code	GBR
[Ref.: #52916]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Human skin

[Ref.: #116349]	Sample type/isolated from	Human, Skin
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X82057 (>99% sequence identity) for Corynebacterium from Microbeatlas

Aquatic Samples	905
Soil Samples	725
Animal Samples	16818
Plant Samples	117
Total Samples	18565

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2888]	Pathogenicity (human)	yes
[Ref.: #2888]	Pathogenicity (animal)	yes
[Ref.: #2888]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #20198]	Biosafety level	2	German classification
[Ref.: #116349]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Corynebacterium amycolatum partial 16S rRNA gene, culture collection CCUG:35685	FN668735	1378	GenBank ENA	43765 tax ID	*
[Ref.: #20218]	Corynebacterium amycolatum partial 16S rRNA gene, type strain CCUG 35685T	HE586271	1304	GenBank ENA	43765 tax ID	*
[Ref.: #20218]	Corynebacterium amycolatum partial 16S-23S rRNA IGS, type strain CCUG 35685T	HE586286	361	GenBank ENA	43765 tax ID	*
[Ref.: #20218]	C.amycolatum 16S ribosomal DNA	X82057	1401	GenBank ENA	43765 tax ID	*
[Ref.: #20218]	Corynebacterium amycolatum strain DSM 6922 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536502	394	GenBank ENA	43765 tax ID	*
[Ref.: #20218]	Corynebacterium amycolatum gene for 16S rRNA, partial sequence	AB470624	544	GenBank ENA	43765 tax ID	*
[Ref.: #20218]	Corynebacterium amycolatum DNA, 16S-23S ribosomal RNA intergenic spacer, partial sequence	AB477423	762	GenBank ENA	43765 tax ID	*
[Ref.: #20218]	Corynebacterium amycolatum 16S rRNA gene, strain NCFB 2768	X84244	1476	GenBank ENA	43765 tax ID	*
[Ref.: #124043]	Corynebacterium amycolatum CCUG 35685 gene for 16S rRNA, partial sequence.	LC752434	570	GenBank ENA	43765 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Corynebacterium amycolatum strain FDAARGOS_1189	43765.128	complete	PATRIC	43765 tax ID	*
[Ref.: #66792]	Corynebacterium amycolatum strain LK23	43765.79	wgs	PATRIC	43765 tax ID	*
[Ref.: #66792]	Corynebacterium amycolatum DSM 6922 Genome Sequencing	2617270851	complete	JGI IMG/M	43765 tax ID	*

GC-content

61 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Corynebacterium amycolatum strain FDAARGOS_1189 strain Not applicable PATRIC: 43765.128
[Ref.: #125438]	gram-positive	gram-positiveⓘ	yes	90.885	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	93.894	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	82.979	no
[Ref.: #125438]	aerobic	aerobicⓘ	yes	71.353	yes
[Ref.: #125438]	thermophile	thermophilicⓘ	no	95.238	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	93	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Corynebacterium amycolatum strain FDAARGOS_1189 PATRIC: 43765.128
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	88.5
[Ref.: #125439]	BacteriaNet	motilityⓘ	no	80.7
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	positive	91.1
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	94.8

Availability in culture collections External links