Corynebacterium amycolatum S160 | Type strain | DSM 6922, ATCC 49368, NCFB 2768

Strain identifier

BacDive ID: 3134

Type strain:

Species: Corynebacterium amycolatum

Strain Designation: S160

Culture col. no.: DSM 6922, ATCC 49368, NCFB 2768

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #2888]	Domain	Bacteria
[Ref.: #2888]	Phylum	Actinobacteria
[Ref.: #2888]	Class	Actinobacteria
[Ref.: #2888]	Order	Actinomycetales
[Ref.: #2888]	Family	Corynebacteriaceae
[Ref.: #2888]	Genus	Corynebacterium
[Ref.: #2888]	Species	Corynebacterium amycolatum
[Ref.: #2888]	Full Scientific Name	Corynebacterium amycolatum Collins et al. 1988
[Ref.: #2888]	Strain Designation	S160
[Ref.: #2888]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
	Last PNU update	25-03-2019 (DD-MM-YYYY)
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Actinobacteria
[Ref.: #20215]	Class	Actinobacteria		Int. J. Syst. Bacteriol. 47:483*
[Ref.: #20215]	Family	Corynebacteriaceae		Int. J. Syst. Bacteriol. 30:235
[Ref.: #20215]	Genus	Corynebacterium	genus (AL)	Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Species	Corynebacterium amycolatum	sp. nov. (VL)	Int. J. Syst. Bacteriol. 38:449
[Ref.: #20215]	Full Scientific Name	Corynebacterium amycolatum Collins et al. 1988

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #20198]	Colony color	Light ivory (1015)
[Ref.: #20198]	Incubation period	10-14 days
[Ref.: #20198]	Cultivation medium used	ISP 2

[Ref.: #20198]	Colony color	Oyster white (1013)
[Ref.: #20198]	Incubation period	10-14 days
[Ref.: #20198]	Cultivation medium used	ISP 4

[Ref.: #20198]	Colony color	Ivory (1014)
[Ref.: #20198]	Incubation period	10-14 days
[Ref.: #20198]	Cultivation medium used	ISP 5

[Ref.: #20198]	Colony color	Sand yellow (1002)
[Ref.: #20198]	Incubation period	10-14 days
[Ref.: #20198]	Cultivation medium used	ISP 6

[Ref.: #20198]	Colony color	Beige (1001)
[Ref.: #20198]	Incubation period	10-14 days
[Ref.: #20198]	Cultivation medium used	ISP 7

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #20198]		NaCl		maximum	7.5	%

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20198]	641	-	-	-	-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #20198]	467	+	-	-	+	-	-	-	-	-	+	-	not determinedn.d.	+	+	+	+	+	+	-	+	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20198]	977	not determinedn.d.	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

	Metabolite	Utilization activity
[Ref.: #20198]	Arabinose	-
[Ref.: #20198]	Cellulose	-
[Ref.: #20198]	Fructose	+
[Ref.: #20198]	Glucose	+
[Ref.: #20198]	Inositol	-
[Ref.: #20198]	Mannitol	-
[Ref.: #20198]	Raffinose	-
[Ref.: #20198]	Rhamnose	-
[Ref.: #20198]	Sucrose	-
[Ref.: #20198]	Xylose	-

[Ref.: #20198]	Multicellular complex forming ability	no
[Ref.: #20198]	Medium Name (multicellularity)	ISP 2

[Ref.: #20198]	Multicellular complex forming ability	no
[Ref.: #20198]	Medium Name (multicellularity)	ISP 4

[Ref.: #20198]	Multicellular complex forming ability	no
[Ref.: #20198]	Medium Name (multicellularity)	ISP 5

[Ref.: #20198]	Multicellular complex forming ability	no
[Ref.: #20198]	Medium Name (multicellularity)	ISP 6

[Ref.: #20198]	Multicellular complex forming ability	no
[Ref.: #20198]	Medium Name (multicellularity)	ISP 7

[Ref.: #2888]	Multimedia content
[Ref.: #2888]	License/Copyright	(C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
[Ref.: #2888]	Intellectual property rights	© Leibniz-Institut DSMZ
[Ref.: #2888]	Multimedia content
[Ref.: #2888]	Caption	Medium 535a 37°C
[Ref.: #2888]	License/Copyright	(C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
[Ref.: #2888]	Intellectual property rights	© Leibniz-Institut DSMZ

[Ref.: #2888]	Murein short key	A31
[Ref.: #2888]	Murein types	A1gamma m-Dpm-direct

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #2888]

Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a), 37°C

[Ref.: #2888]

Culture medium growth

[Ref.: #2888]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #20198]

Culture medium

ISP 2

[Ref.: #20198]

Culture medium growth

[Ref.: #20198]

Culture medium composition

expand / minimize

[Ref.: #20198]

Culture medium

ISP 4

[Ref.: #20198]

Culture medium growth

[Ref.: #20198]

Culture medium composition

expand / minimize

[Ref.: #20198]

Culture medium

ISP 5

[Ref.: #20198]

Culture medium growth

[Ref.: #20198]

Culture medium composition

expand / minimize

[Ref.: #20198]

Culture medium

ISP 6

[Ref.: #20198]

Culture medium growth

[Ref.: #20198]

Culture medium composition

expand / minimize

[Ref.: #20198]

Culture medium

ISP 7

[Ref.: #20198]

Culture medium growth

[Ref.: #20198]

Culture medium composition

expand / minimize

[Ref.: #34280]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #34280]

Culture medium growth

[Ref.: #34280]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #2888]	growth	37 ̊C
[Ref.: #20198]	optimum	28 ̊C
[Ref.: #34280]	growth	37 ̊C

[Ref.: #2888]	Temperature range	mesophilic
[Ref.: #20198]	Temperature range	mesophilic
[Ref.: #34280]	Temperature range	mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #2888]

Sample type/isolated from

human skin

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #2888]	Pathogenicity (human)	yes
[Ref.: #2888]	Pathogenicity (animal)	yes
[Ref.: #2888]	Biosafety level	2	Risk group (German classification)
[Ref.: #20198]	Biosafety level	2	German classification

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #2888]

GC-content

61.0 mol%

thermal denaturation, midpoint method (Tm)

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Corynebacterium amycolatum strain ATCC 49368 60 kDa chaperonin (cpn60) gene, partial cds	AY691245	552	43765	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Corynebacterium amycolatum partial 16S rRNA gene, culture collection CCUG:35685	FN668735	1378	43765	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Corynebacterium amycolatum partial 16S rRNA gene, type strain CCUG 35685T	HE586271	1304	43765	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Corynebacterium amycolatum partial 16S-23S rRNA IGS, type strain CCUG 35685T	HE586286	361	43765	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Corynebacterium amycolatum partial rpoB gene for RNA polymerase beta subunit, type strain CCUG 35685T	HE586300	407	43765	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Corynebacterium amycolatum partial hsp65 gene for 65 kDa heat shock protein, type strain CCUG 35685T	HE586304	314	43765	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Corynebacterium amycolatum strain CIP 103452 RpoB (rpoB) gene, partial cds	AY492241	3435	43765	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Corynebacterium amycolatum strain CIP 103452 65 kDa heat shock protein (hsp65) gene, partial cds	DQ782923	422	43765	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	C.amycolatum 16S ribosomal DNA	X82057	1401	43765	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Corynebacterium amycolatum strain DSM 6922 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536502	394	43765	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Corynebacterium amycolatum gyrB gene for DNA gyrase B subunit, partial cds, strain JCM 7447	AB014278	1281	43765	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Corynebacterium amycolatum SOD gene for superoxide dismutase protein, partial cds	AB469807	468	43765	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Corynebacterium amycolatum rpoB gene for rpoB protein, partial cds, strain: JCM 7447	AB469960	318	43765	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Corynebacterium amycolatum gene for 16S rRNA, partial sequence	AB470624	544	43765	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Corynebacterium amycolatum DNA, 16S-23S ribosomal RNA intergenic spacer, partial sequence	AB477423	762	43765	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Corynebacterium amycolatum gyrase subunit A (gyrA) gene, partial cds	AY647174	212	43765	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Corynebacterium amycolatum DNA topoisomerase IV subunit A (parC) gene, partial cds	AY647175	141	43765	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Corynebacterium amycolatum 16S rRNA gene, strain NCFB 2768	X84244	1476	43765	*

Availability in culture collectionsStrain availability

[Ref.: #2888]

Culture collection no.

DSM 6922, ATCC 49368, NCFB 2768

[Ref.: #2888]

Strain history

<- NCFB <- D. Jones <- R.R. Marples, S160

[Ref.: #20218]

Associated Passport(s) in StrainInfo

48859, 48857, 48861

References

#2888

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6922

#20198

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34280 ; Curators of the CIP;
* These References are textmined

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