Name: Corynebacterium variabile AC 256
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3125

Type strain:

Species: Corynebacterium variabile

Strain Designation: AC 256

Culture col. no.: DSM 20536, ATCC 33010, IFO 14757, NBRC 14757, NCDO 2097, CCUG 23851, CCM 3383, LMG 19059, JCM 1377, BCRC 14862, CECT 4164, IAM 12549, KCTC 9102

Strain history: K. Suzuki CNF 057 <-- W. H. J. Crombach AC 256.

NCBI tax ID(s): 1727 (species)

SI-ID 217648

LMG 19059, ATCC 33010, CCM 3383, CCUG 23851, IFO 14757, NCDO 2097, JCM 1377, CCRC 14862, IAM 12549, NCIMB 702097, CECT 4164, NCFB 2097, IMET 11242, KCTC 9102, NBRC 14757, BCRC 14862, HAMBI 2051, LMG 22561, DSM 20536

Other strains from Corynebacterium variabile

C. variabile FK 31, DSM 20132, ATCC 15753, CCM ... (type strain)
C. variabile AC 263, DSM 20537
C. variabile DSM 44702, CIP 107183, DPC 5310, ...
C. variabile SF002542(FSU),
C. variabile SF002543(FSU),

Section

Corynebacterium variabile AC 256 is a mesophilic bacterium that was isolated from meshanger cheese.

mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium variabile**
	Full Scientific Name (LPSN)	Corynebacterium variabile corrig. (Müller 1961) Collins 1987

	Synonym	Caseobacter polymorphus
	Synonym	Arthrobacter variabilis
	Synonym	Corynebacterium mooreparkense
	Synonym	Corynebacterium variabilis

Information on morphological and physiological properties Morphology

Multimedia content

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Caption:

Medium 535a 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8766]			growth	28 °C
[Ref.: #67770]			growth	30 °C

[Ref.: #8766]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8766]	Murein short key	A31
[Ref.: #8766]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #47862]

Fatty acid	Percentage	ECL
C16:0	23.8	16
C17:1 ω9c	18.7	16.773
C18:1 ω9c	17.3	17.769
C18:2 ω6,9c/C18:0 ANTE	11.4	17.724
C17:0	9.9	17
C17:0 anteiso	5.4	16.722
C14:0	3.9	14
TBSA 10Me18:0	3.5	18.392
C18:0	3.2	18
C15:0	1.4	15
C17:1 ω5c ISO	0.6	16.461
C16:1 ω9c	0.6	15.774
C20:4 ω6,9,12,15c	0.4	19.395

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #47862]	907	-	-	-	-	-	-	-	-	+	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	+
[Ref.: #47862]	908	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #47862]	1552	-	-	+	+	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8766]	Sample type/isolated from	meshanger cheese

[Ref.: #47862]	Sample type/isolated from	Meshanger cheese

[Ref.: #67770]	Sample type/isolated from	Meshanger cheese
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Dairy product

What are isolation sources categories?

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Global distribution of 16S sequence AJ222816 (>99% sequence identity) for Corynebacterium from Microbeatlas

Aquatic Samples	2885
Soil Samples	1425
Animal Samples	11599
Plant Samples	671
Total Samples	16580

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Corynebacterium variabilis (strain DSM 20536) 16S rRNA gene	AJ222816	1438	ENA	1727 tax ID	*
[Ref.: #20218]	Corynebacterium variabilis 16S rRNA (part.)	X53185	1458	ENA	1727 tax ID	*

[Ref.: #8766]	GC-content	66.1 mol%
[Ref.: #67770]	GC-content	63.9 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	66.1 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #8766]

Culture collection no.

DSM 20536, ATCC 33010, IFO 14757, NBRC 14757, NCDO 2097, CCUG 23851, CCM 3383, LMG 19059, JCM 1377, BCRC 14862, CECT 4164, IAM 12549, KCTC 9102

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SI-ID 217648

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Intraspecific variation of unusual phospholipids from Corynebacterium spp. containing a novel fatty acid.	Niepel T, Meyer H, Wray V, Abraham WR	J Bacteriol	10.1128/JB.180.17.4650-4657.1998	1998	*

References

#8766

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20536

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#47862 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 23851
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72642

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID217648.1 )

* These data were automatically processed and therefore are not curated

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