Name: Corynebacterium pseudotuberculosis E23
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3113

Type strain:

Species: Corynebacterium pseudotuberculosis

Strain Designation: E23

Culture col. no.: DSM 20689, ATCC 19410, NCTC 3450, CCUG 2806, LMG 19054, CIP 102968, JCM 9389, BCRC 14872, CCM 3428, CECT 808, IFO 15363, NBRC 15363, PCM 2052

Strain history: CIP <- 1987, NCTC <- 1931, G.F. Petrie: strain E23 <- J.B. Buxton, Corynebacterium ovis

NCBI tax ID(s): 1719 (species)

SI-ID 12980

LMG 19054, ATCC 19410, CCUG 2806, CIP 102968, IFO 15363, NCTC 3450, JCM 9389, CCM 3428, CCRC 14872, CECT 808, NBRC 15363, BCRC 14872, CNCTC 5692, DSM 20689

Other strains from Corynebacterium pseudotuberculosis

Section

Corynebacterium pseudotuberculosis E23 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from infected gland of sheep.

Gram-positive
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium pseudotuberculosis**
	Full Scientific Name (LPSN)	Corynebacterium pseudotuberculosis (Buchanan 1911) Eberson 1918 (Approved Lists 1980)

Synonym

"Bacillus pseudotuberculosis"

Information on morphological and physiological properties Morphology

[Ref.: #122002]	Gram stain	positive

[Ref.: #122002]	Cell shape	rod-shaped

[Ref.: #122002]	Motility	no

[Ref.: #18770]	Incubation period	10-14 days
[Ref.: #18770]	Cultivation medium used	ISP 2

[Ref.: #18770]	Incubation period	10-14 days
[Ref.: #18770]	Cultivation medium used	ISP 3

[Ref.: #18770]	Incubation period	10-14 days
[Ref.: #18770]	Cultivation medium used	ISP 4

[Ref.: #18770]	Incubation period	10-14 days
[Ref.: #18770]	Cultivation medium used	ISP 5

[Ref.: #18770]	Incubation period	10-14 days
[Ref.: #18770]	Cultivation medium used	ISP 6

[Ref.: #18770]	Incubation period	10-14 days
[Ref.: #18770]	Cultivation medium used	ISP 7

[Ref.: #18770]	Multicellular complex forming ability	no
[Ref.: #18770]	Medium Name (multicellularity)	ISP 2

[Ref.: #18770]	Multicellular complex forming ability	no
[Ref.: #18770]	Medium Name (multicellularity)	ISP 3

[Ref.: #18770]	Multicellular complex forming ability	no
[Ref.: #18770]	Medium Name (multicellularity)	ISP 4

[Ref.: #18770]	Multicellular complex forming ability	no
[Ref.: #18770]	Medium Name (multicellularity)	ISP 5

[Ref.: #18770]	Multicellular complex forming ability	no
[Ref.: #18770]	Medium Name (multicellularity)	ISP 6

[Ref.: #18770]	Multicellular complex forming ability	no
[Ref.: #18770]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18770]

Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 6

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 7

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Culture medium growth

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Culture medium composition

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Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #9010]

Culture medium composition

expand / minimize

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Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #9010]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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[Ref.: #38112]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38112]

Culture medium growth

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Culture medium composition

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[Ref.: #122002]

Culture medium

CIP Medium 72

[Ref.: #122002]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

CIP Medium 3

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #9010]	growth	37 °C
[Ref.: #18770]	optimum	37 °C
[Ref.: #38112]	growth	37 °C
[Ref.: #44589]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #9010]	Temperature range	mesophilic
[Ref.: #18770]	Temperature range	mesophilic
[Ref.: #38112]	Temperature range	mesophilic
[Ref.: #44589]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44589]

Oxygen tolerance

microaerophile

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44589]

Fatty acid	Percentage	ECL
C18:2 ω6,9c/C18:0 ANTE	36.9	17.724
C16:0	22.4	16
C18:1 ω9c	19.6	17.769
C18:0	18.7	18
C17:1 ISO I/C16:0 DMA	2.4	16.481

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18770]	201	-	-	-	+	-	-	-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.
[Ref.: #9010]	4221	-	-	-	+	-	-	+	-	-	+	-	-	+	+	-	-	+/-	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18770]	335	not determinedn.d.	+	+	+	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #9010]	Sample type/isolated from	infected gland of sheep

[Ref.: #44589]	Sample type/isolated from	Sheep gland infection
[Ref.: #44589]	Country	South America
[Ref.: #44589]	Continent	Middle and South America

[Ref.: #67770]	Sample type/isolated from	Infected gland of sheep
[Ref.: #67770]	Country	South America

[Ref.: #122002]	Sample type/isolated from	Animal, Sheep, infected gland
[Ref.: #122002]	Country	South America
[Ref.: #122002]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body-Site	-	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X81916 (>99% sequence identity) for Corynebacterium from Microbeatlas

Aquatic Samples	2458
Soil Samples	2353
Animal Samples	24362
Plant Samples	894
Total Samples	30067

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18770]	Biosafety level	2	Risk group (German classification)
[Ref.: #9010]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #122002]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #9010]	Corynebacterium pseudotuberculosis (strain ATCC 19410) gene for 16S ribosomal RNA	D38579	1472	ENA	1719 tax ID
[Ref.: #20218]	C.pseudotuberculosis 16S rRNA gene (CIP 102968T)	X81916	1397	ENA	1719 tax ID	*
[Ref.: #20218]	Corynebacterium pseudotuberculosis strain DSM 20689 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536509	377	ENA	1719 tax ID	*
[Ref.: #20218]	Corynebacterium pseudotuberculosis 16S rRNA gene, strain NCTC 3450	X84255	1446	ENA	1719 tax ID	*
[Ref.: #20218]	Corynebacterium pseudotuberculosis strain ATCC 19410 16S ribosomal RNA gene, partial sequence	GQ118342	1442	ENA	1719 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Corynebacterium pseudotuberculosis DSM 20689	GCA_003634885	contig	GenBank	1719 tax ID
[Ref.: #66792]	Corynebacterium pseudotuberculosis ATCC 19410	GCA_002155265	complete	GenBank	1719 tax ID	*
[Ref.: #66792]	Corynebacterium pseudotuberculosis strain ATCC 19410	1719.2381	complete	PATRIC	1719 tax ID	*
[Ref.: #66792]	Corynebacterium pseudotuberculosis strain DSM 20689	1719.2642	wgs	PATRIC	1719 tax ID	*
[Ref.: #66792]	Corynebacterium pseudotuberculosis DSM 20689	2756170169	draft	JGI IMG/M	1719 tax ID	*
[Ref.: #66792]	Corynebacterium pseudotuberculosis ATCC 19410	2872045035	complete	JGI IMG/M	1719 tax ID	*

[Ref.: #67770]	GC-content	52.2 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	51.8 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium pseudotuberculosis ATCC 19410 NCBI: GCA_002155265.1
[Ref.: #69480]	flagellated	no	97.594	no
[Ref.: #69480]	gram-positive	yes	94.119	no
[Ref.: #69480]	anaerobic	no	99.013	no
[Ref.: #69480]	halophile	yes	72.539	no
[Ref.: #69480]	spore-forming	no	93.439	no
[Ref.: #69480]	glucose-util	yes	89.043	no
[Ref.: #69480]	aerobic	no	83.587	yes
[Ref.: #69480]	thermophile	no	95.537	yes
[Ref.: #69480]	motile	no	93.963	no
[Ref.: #69480]	glucose-ferment	yes	70.128	no

Availability in culture collections External links

[Ref.: #9010]

Culture collection no.

DSM 20689, ATCC 19410, NCTC 3450, CCUG 2806, LMG 19054, CIP 102968, JCM 9389, BCRC 14872, CCM 3428, CECT 808, IFO 15363, NBRC 15363, PCM 2052

[Ref.: #72631]

SI-ID 12980

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp. nov., Corynebacterium pseudopelargi sp. nov., and Corynebacterium gerontici sp. nov.	Busse HJ, Kleinhagauer T, Glaeser SP, Spergser J, Kampfer P, Ruckert C	Int J Syst Evol Microbiol	10.1099/ijsem.0.003580	2019	*
Pathogenicity	Lawsonia inermis L. leaves aqueous extract as a natural antioxidant and antibacterial product.	Pasandi Pour A, Farahbakhsh H	Nat Prod Res	10.1080/14786419.2019.1569006	2019	*
Phylogeny	Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi.	Almeida S, Dorneles EMS, Diniz C, Abreu V, Sousa C, Alves J, Carneiro A, Bagano P, Spier S, Barh D, Lage AP, Figueiredo H, Azevedo V	BMC Vet Res	10.1186/s12917-017-1210-5	2017	*
Phylogeny	Molecular epidemiology of Corynebacterium pseudotuberculosis isolated from horses in California.	Haas DJ, Dorneles EM, Spier SJ, Carroll SP, Edman J, Azevedo VA, Heinemann MB, Lage AP	Infect Genet Evol	10.1016/j.meegid.2016.12.011	2016	*
Phylogeny	Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.	Dorneles EM, Santana JA, Ribeiro D, Dorella FA, Guimaraes AS, Moawad MS, Selim SA, Garaldi AL, Miyoshi A, Ribeiro MG, Gouveia AM, Azevedo V, Heinemann MB, Lage AP	PLoS One	10.1371/journal.pone.0098758	2014	*
Enzymology	Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR.	Dorneles EM, Santana JA, Andrade GI, Santos EL, Guimaraes AS, Mota RA, Santos AS, Miyoshi A, Azevedo V, Gouveia AM, Lage AP, Heinemann MB	Genet Mol Res	10.4238/2012.August.6.9	2012	*
Phylogeny	Corynebacterium mustelae sp. nov., isolated from a ferret with lethal sepsis.	Funke G, Frodl R, Bernard KA	Int J Syst Evol Microbiol	10.1099/ijs.0.010942-0	2009	*
	Caseous Lymphadenitis Caused by Corynebacterium pseudotuberculosis in Alpine Chamois (Rupicapra r. rupicapra): a Review of 98 Cases.	Domenis L, Spedicato R, Pepe E, Orusa R, Robetto S	J Comp Pathol	10.1016/j.jcpa.2018.04.003	2018	*

References

#9010

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20689

#18770

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38112 ; Curators of the CIP;
#44589 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 2806

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#72631

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12980.1 )

#122002 Collection of Institut Pasteur ; Curators of the CIP; CIP 102968
* These data were automatically processed and therefore are not curated

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Corynebacterium pseudotuberculosis ATCC 19410

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