Polaromonas hydrogenivorans (DSM 17735, CIP 109586, NRRL B-41369)

Name: Polaromonas hydrogenivorans (DSM 17735, CIP 109586, NRRL B-41369)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2983

Type strain

Strain Designation DQ094183

Culture col. no. DSM 17735 CIP 109586 NRRL B-41369 DQ094183 KCTC 42857

NCBI tax ID(s) 335476

Special Collections DSMZ CIP Literature strains

History DSMZ <- M. Sizova CIP <- 2007, DSMZ <- M. Sizova, Stevens Inst., Hoboken, USA: strain DQ094183

Links

Polaromonas hydrogenivorans DQ094183 is an aerobe, chemolithotroph, Gram-negative bacterium that forms circular colonies and was isolated from seasonally frozen forest soil.

Gram-negative coccus-shaped colony-forming aerobe chemolithotroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 297298

KCTC 42857,DSM 17735,NRRL B-41369,CIP 109586

@ref 20215

Last LPSN update 2026-05-29 08:25:36

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Comamonadaceae

Genus Polaromonas

Species Polaromonas hydrogenivorans

Full scientific name Polaromonas hydrogenivorans Sizova and Panikov 2007

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Confidence
23089	negative	0.8-2.8 µm	coccus-shaped
120002	negative		oval-shaped
125439	negative			99.516

Colony morphology

@ref	Colony size	Colony color	Colony shape	Medium used
23089	0.5-2 mm	white	circular	mineral agar
23089	2.0-5.0 mm	shiny beige	circular	nutrient agar

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6927	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23089	mineral agar
23089	Nutrient agar (NA)
120002	CIP Medium 328	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6927	positive	growth	10
23089	positive	growth	0.0-25.0
23089	negative	growth	28.0
23089	positive	optimum	15.0-20.0

Culture pH

@ref	Ability	Type	PH
23089	positive	optimum	6.0-7.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23089	aerobe
120002	obligate aerobe
125439	obligate aerobe	96.334

Nutrition type

@ref	Type
23089	chemolithotroph
23089	organotroph

Spore formation

@ref	Spore formation	Confidence
23089
125439		99.814

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23089	62064 ChEBI	2,3-butanediol	+	carbon source
23089	64552 ChEBI	2-hydroxybutyrate	+	carbon source
23089	16763 ChEBI	2-oxobutanoate	+	carbon source
23089	28644 ChEBI	2-oxopentanoate	+	carbon source
23089	37054 ChEBI	3-hydroxybutyrate	+	carbon source
23089	16724 ChEBI	4-hydroxybutyrate	+	carbon source
23089	18101 ChEBI	4-hydroxyphenylacetic acid	+	carbon source
23089	30089 ChEBI	acetate	+	carbon source
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
23089	40585 ChEBI	alpha-cyclodextrin	+	carbon source
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23089	73706 ChEBI	bromosuccinate	+	carbon source
23089	16383 ChEBI	cis-aconitate	+	carbon source
23089	15570 ChEBI	D-alanine	+	carbon source
23089	18333 ChEBI	D-arabitol	-	carbon source
23089	15824 ChEBI	D-fructose	+	carbon source
23089	12931 ChEBI	D-galactonate	-	carbon source
23089	12936 ChEBI	D-galactose	+	carbon source
23089	18024 ChEBI	D-galacturonic acid	+	carbon source
23089	30612 ChEBI	D-glucarate	-	carbon source
23089	8391 ChEBI	D-gluconate	+	carbon source
23089	17784 ChEBI	D-glucosaminic acid	-	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
23089	14314 ChEBI	D-glucose 6-phosphate	+	carbon source
23089	15748 ChEBI	D-glucuronate	+	carbon source
23089	16899 ChEBI	D-mannitol	-	carbon source
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
23089	27605 ChEBI	D-psicose	+	carbon source
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
23089	23652 ChEBI	dextrin	-	carbon source
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
23089	16236 ChEBI	ethanol	+	carbon source
23089	16000 ChEBI	ethanolamine	-	carbon source
23089	15740 ChEBI	formate	+	carbon source
23089	16865 ChEBI	gamma-aminobutyric acid	+	carbon source
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
23089	28066 ChEBI	gentiobiose	-	carbon source
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
23089	17234 ChEBI	glucose	+	carbon source
23089	17754 ChEBI	glycerol	-	carbon source
23089	28087 ChEBI	glycogen	+	carbon source
23089	73804 ChEBI	glycyl L-aspartic acid	-	carbon source
23089	73784 ChEBI	glycyl-l-glutamate	-	carbon source
23089	17240 ChEBI	itaconate	+	carbon source
23089	30849 ChEBI	L-arabinose	-	carbon source
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
23089	29991 ChEBI	L-aspartate	+	carbon source
23089	18183 ChEBI	L-pyroglutamic acid	-	carbon source
23089	62345 ChEBI	L-rhamnose	+	carbon source
23089	17115 ChEBI	L-serine	+	carbon source
23089	16857 ChEBI	L-threonine	+	carbon source
23089	6359 ChEBI	lactulose	-	carbon source
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
23089	15792 ChEBI	malonate	+	carbon source
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
23089	28053 ChEBI	melibiose	+	carbon source
23089	320055 ChEBI	methyl beta-D-glucopyranoside	+	carbon source
23089	51850 ChEBI	methyl pyruvate	+	carbon source
23089	75146 ChEBI	monomethyl succinate	+	carbon source
23089	17268 ChEBI	myo-inositol	-	carbon source
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
120002	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
120002	16301 ChEBI	nitrite	-	reduction
23089	16452 ChEBI	oxaloacetate	-	carbon source
23089	26490 ChEBI	quinate	+	carbon source
23089	16634 ChEBI	raffinose	+	carbon source
23089	15963 ChEBI	ribitol	-	carbon source
23089	41865 ChEBI	sebacic acid	+	carbon source
23089	143136 ChEBI	succinamate	+	carbon source
23089	17992 ChEBI	sucrose	+	carbon source
23089	27082 ChEBI	trehalose	+	carbon source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
23089	32528 ChEBI	turanose	+	carbon source
23089	53423 ChEBI	tween 40	+	carbon source
23089	53426 ChEBI	tween 80	+	carbon source
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE
23089	27248 ChEBI	urocanic acid	-	carbon source
23089	17151 ChEBI	xylitol	+	carbon source

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.	Resistance conc.
23089	17833	gentamicin	10 µg (disc)
23089	7507	neomycin		30 µg (disc)
23089	28368	novobiocin		30 µg (disc)
23089	17076	streptomycin	10 µg (disc)
23089	27902	tetracycline	30 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
120002	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
23089	catalase	+	1.11.1.6
120002	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
23089	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
120002	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
120002	urease	-	3.5.1.5
68369	urease	+	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120002	-	+	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
6927	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition
6927	seasonally frozen forest soil	Alaska, Fairbanks	USA	USA	North America
23089						mineral medium	containing (l-1): 2 g K2HPO4, 1.5 g NaH2PO4, 1 g (NH4)2SO4, 0.1 g CaCl2, 0.4 g MgSO4, 0.01 g Na-EDTA, 1x10-3 g FeCl3.6H2O, 2x10-4 g KI, 2x10-4 g CoCl2.6H2O, 8x10-4 g MnCl2.4H2O, 8x10-4 g ZnSO4, 1x10-4 g H3BO3 , 1x10-4 g Na2MoO4.2H2O, 1x10-4 g CuCl2 and 10-4 g NiCl2.6H2O
120002	Environment, Soil, seasonally frozen forest	Fairbanks, Alaska	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence DQ094183 (>99% sequence identity) for Polaromonas from Microbeatlas

Aquatic Samples	1647
Soil Samples	683
Animal Samples	380
Plant Samples	107
Total Samples	2817

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6927	1	Risk group (German classification) According to TRBA 466
120002	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	57455 assembly for Polaromonas hydrogenivorans DSM 17735	complete	GCA_040105105	335476	92.51

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6927	Polaromonas hydrogenivorans strain DSM 17735 16S ribosomal RNA gene, partial sequence	DQ094183	1384		335476

GC content

@ref	GC-content (mol%)	Method
6927	62.5
23089	62.5	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: 57455 assembly for Polaromonas hydrogenivorans DSM 17735
NCBI: GCA_040105105

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.33	no
125439	gram_stain	BacteriaNetⓘ	negative	99.52	no
125439	motility	BacteriaNetⓘ	yes	75.35	no
125439	spore_formation	BacteriaNetⓘ	no	99.81	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polaromonas hydrogenivorans sp. nov., a psychrotolerant hydrogen-oxidizing bacterium from Alaskan soil.	Sizova M, Panikov N	Int J Syst Evol Microbiol	10.1099/ijs.0.64350-0	2007

References

#6927	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17735
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23089	Maria Sizova,Nicolai Panikov: Polaromonas hydrogenivorans sp. nov., a psychrotolerant hydrogen-oxidizing bacterium from Alaskan soil. IJSEM 57: 616 - 619 2007 ( DOI 10.1099/ijs.0.64350-0 , PubMed 17329795 )
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120002	Collection of Institut Pasteur ; Curators of the CIP; CIP 109586
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Polaromonas hydrogenivorans (DSM 17735, CIP 109586, NRRL B-41369)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_040105105

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media