Name: Achromobacter xylosoxidans subsp. xylosoxidans KM543
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 297

Type strain:

Species: Achromobacter xylosoxidans subsp. xylosoxidans

Strain Designation: KM543

Culture col. no.: DSM 2402, ATCC 27061, DSM 10346, NRRL 84082, CCUG 56438, CIP 71.32, NCTC 10807, LMG 1863, CCM 2741, IFO 15126, NCIMB 12033, JCM 9659, CCUG 12689, CECT 467, CECT 927, IAM 12684, NBRC 15126, NRRL B-4082

Strain history: R. Hugh 2838.

NCBI tax ID(s): 85698 (species)

Other strains from Achromobacter xylosoxidans subsp. xylosoxidans

A. xylosoxidans subsp. xylosoxidans DSM 26050, CCUG 48584
A. xylosoxidans subsp. xylosoxidans DSM 26051, CCUG 48684
A. xylosoxidans subsp. xylosoxidans R-9501, DSM 26088, CCUG 42363
A. xylosoxidans subsp. xylosoxidans DSM 26073, CCUG 48386
A. xylosoxidans subsp. xylosoxidans DSM 26089, CCUG 45179
A. xylosoxidans subsp. xylosoxidans DSM 26101, CCUG 41513
A. xylosoxidans subsp. xylosoxidans DSM 26102, CCUG 56600
A. xylosoxidans subsp. xylosoxidans DSM 26161, CCUG 48331

Section

Achromobacter xylosoxidans subsp. xylosoxidans KM543 is an aerobe, mesophilic bacterium that was isolated from ear discharge.

aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Alcaligenaceae*
	Genus	*Achromobacter*
	Species	Achromobacter xylosoxidans subsp. xylosoxidans
	Full Scientific Name (PNU)	Achromobacter xylosoxidans subsp. xylosoxidans (Yabuuchi and Yano 1981) Yabuuchi et al. 1998

Synonym

Alcaligenes xylosoxidans subsp. xylosoxidans

Information on morphological and physiological properties Morphology

[Ref.: #1075]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #36489]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #36489]

Culture medium growth

[Ref.: #36489]

Culture medium composition

expand / minimize

[Ref.: #1075]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #1075]

Culture medium growth

[Ref.: #1075]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #1075]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #1075]	growth	30 °C
[Ref.: #36489]	growth	30 °C
[Ref.: #60956]	growth	37 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #1075]	Temperature range	mesophilic
[Ref.: #36489]	Temperature range	mesophilic
[Ref.: #60956]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #60956]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE, API 20E
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE, API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE, API 20E
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68369]	indole ChEBI	* from API 20NE, API 20E
[Ref.: #68368]	nitrite ChEBI	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68369]		indole ChEBI		-	* from API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE, API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #1075]	catalase	+	1.11.1.6
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #1075]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE, API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE, API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #1075]	14246	-	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #1075]	21987	+	-	-	-	-	-	-	-	+	-	+/-	-	-	-	+	+	+	+	+	+	+
[Ref.: #1075]	20958	+	-	-	-	-	-	-	-	+	-	-	-	-	-	+	+	+	+	+	+	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1075]	Sample type/isolated from	ear discharge

[Ref.: #60956]	Sample type/isolated from	Human ear
[Ref.: #60956]	Sampling date	1969
[Ref.: #60956]	Geographic location (country and/or sea, region)	Osaka
[Ref.: #60956]	Country	Japan
[Ref.: #60956]	Country ISO 3 Code	JPN
[Ref.: #60956]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Ear discharge
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Organ	#Ear
#Host Body Product	#Fluids	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Y14908 (>99% sequence identity) for Achromobacter from Microbeatlas

Aquatic Samples	362
Soil Samples	273
Animal Samples	742
Plant Samples	143
Total Samples	1520

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1075]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1075]	Achromobacter xylosoxidans 16S rRNA gene, type strain DSM 10346	Y14908	1495	ENA	85698 tax ID
[Ref.: #67770]	Achromobacter xylosoxidans gene for 16S ribosomal RNA	D88005	1462	ENA	85698 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Achromobacter xylosoxidans NCTC10807	GCA_001457475	complete	GenBank	85698 tax ID	*
[Ref.: #66792]	Achromobacter xylosoxidans NBRC 15126 = ATCC 27061 NBRC 15126 = ATCC 27061	GCA_000508285	complete	GenBank	1216976 tax ID	*
[Ref.: #66792]	Achromobacter xylosoxidans NBRC 15126 = ATCC 27061	1216976.8	wgs	PATRIC	1216976 tax ID	*
[Ref.: #66792]	Achromobacter xylosoxidans NBRC 15126 = ATCC 27061	1216976.3	complete	PATRIC	1216976 tax ID	*
[Ref.: #66792]	Achromobacter xylosoxidans strain FDAARGOS_789 strain Not applicable	85698.244	complete	PATRIC	85698 tax ID	*
[Ref.: #66792]	Achromobacter xylosoxidans strain NCTC10807	85698.28	complete	PATRIC	85698 tax ID	*

[Ref.: #1075]	GC-content	69.2 mol%
[Ref.: #67770]	GC-content	57.6 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #1075]

Culture collection no.

DSM 2402, ATCC 27061, DSM 10346, NRRL 84082, CCUG 56438, CIP 71.32, NCTC 10807, LMG 1863, CCM 2741, IFO 15126, NCIMB 12033, JCM 9659, CCUG 12689, CECT 467, CECT 927, IAM 12684, NBRC 15126, NRRL B-4082

[Ref.: #20218]

Associated Passport(s) in StrainInfo

51746, 51741, 185639, 157985

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Nosocomial colonization and infection by Achromobacter xylosoxidans.	Reverdy ME, Freney J, Fleurette J, Coulet M, Surgot M, Marmet D, Ploton C	J Clin Microbiol	10.1128/jcm.19.2.140-143.1984	1984	*
Phylogeny	Emendation of genus Achromobacter and Achromobacter xylosoxidans (Yabuuchi and Yano) and proposal of Achromobacter ruhlandii (Packer and Vishniac) comb. nov., Achromobacter piechaudii (Kiredjian et al.) comb. nov., and Achromobacter xylosoxidans subsp. denitrificans (Ruger and Tan) comb. nov.	Yabuuchi E, Kawamura Y, Kosako Y, Ezaki T	Microbiol Immunol	10.1111/j.1348-0421.1998.tb02306.x	1998	*
Enzymology	Cytoplasmic expression of the Achromobacter xylosoxidans blue copper nitrite reductase in Escherichia coli and characterisation of the recombinant protein.	Ho WH, Ooi BL, Jorgensen AM, Borg L, Jespersen LL, Christensen HE	Protein Expr Purif	10.1016/j.pep.2003.08.010	2003	*
Phylogeny	Parapusillimonas granuli gen. nov., sp. nov., isolated from granules from a wastewater-treatment bioreactor.	Kim YJ, Kim MK, Im WT, Srinivasan S, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.009720-0	2009	*
Pathogenicity	Genomic insights into intrinsic and acquired drug resistance mechanisms in Achromobacter xylosoxidans.	Hu Y, Zhu Y, Ma Y, Liu F, Lu N, Yang X, Luan C, Yi Y, Zhu B	Antimicrob Agents Chemother	10.1128/AAC.04260-14	2014	*
Phylogeny	Achromobacter aloeverae sp. nov., isolated from the root of Aloe vera (L.) Burm.f.	Kuncharoen N, Muramatsu Y, Shibata C, Kamakura Y, Nakagawa Y, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.001566	2017	*
Phylogeny	Sulfur oxidation by Achromobacter xylosoxidans strain wsp05 reveals ecological widening over which thiotrophs are distributed.	Jadhav K, Jadhav I	World J Microbiol Biotechnol	10.1007/s11274-017-2359-6	2017	*
Phylogeny	Achromobacter aestuarii sp. nov., Isolated from an Estuary.	Kim SC, Chung SO, Lee HJ	Curr Microbiol	10.1007/s00284-020-02231-9	2020	*

References

#1075

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2402

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36489 ; Curators of the CIP;
#60956 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56438

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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