Butyricicoccus pullicaecorum (DSM 23266, CCUG 55265, LMG 24109)

Name: Butyricicoccus pullicaecorum (DSM 23266, CCUG 55265, LMG 24109)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2871

Type strain

Strain Designation 25-3

Culture col. no. DSM 23266 CCUG 55265 LMG 24109 25-3

NCBI tax ID(s) 1121128 501571

Special Collections DSMZ CCUG Literature strains

History DSMZ <- E. Moore, CCUG; CCUG 55265 <- V. Eeckhaut, Lab. Microbiology, Ghent University, Belgium; 25-3 {2006}

Links

Butyricicoccus pullicaecorum 25-3 is an anaerobe, Gram-positive, coccus-shaped bacterium that was isolated from cecal content .

Gram-positive coccus-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 354037

LMG 24109,CCUG 55265,DSM 23266

@ref 20215

Last LPSN update 2026-05-29 08:25:34

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Oscillospiraceae

Genus Butyricicoccus

Species Butyricicoccus pullicaecorum

Full scientific name Butyricicoccus pullicaecorum Eeckhaut et al. 2008

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Motility
32584	positive	1.2-1.5 µm	coccus-shaped

Colony morphology

60513

Incubation period2 days

Pigmentation

32584

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16766	CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)		Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water
16766	PY + X MEDIUM (DSMZ Medium 104b)		Medium recipe at MediaDive	Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
16766	positive	growth	37
32584	positive	growth	42
32584	positive	optimum	42
60513	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
16766	anaerobe
32584	anaerobe
60513	anaerobe
125439	obligate anaerobe	97.324

Spore formation

32584

Spore formationno

Observation

32584

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32584	17057 ChEBI	cellobiose	+	carbon source
32584	4853 ChEBI	esculin	+	hydrolysis
32584	17234 ChEBI	glucose	+	carbon source
32584	17306 ChEBI	maltose	+	carbon source
32584	37684 ChEBI	mannose	+	carbon source
32584	17814 ChEBI	salicin	+	carbon source
32584	17992 ChEBI	sucrose	+	carbon source
32584	27082 ChEBI	trehalose	+	carbon source

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Birds	#Chicken
#Host Body Product	#Gastrointestinal tract	#Caecal content

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
16766	cecal content (4-week-old broiler chicken)		Maldegem	Belgium	BEL	Europe
60513	Chicken feces,4-week old broiler	2006-11-24	Gent	Belgium	BEL	Europe

Taxonmaps

69479

Global distribution of 16S sequence EU410376 (>99% sequence identity) for Butyricicoccus pullicaecorum subclade from Microbeatlas

Aquatic Samples	195
Soil Samples	391
Animal Samples	28431
Plant Samples	80
Total Samples	29097

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16766	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2585428076 annotated assembly for Butyricicoccus pullicaecorum DSM 23266	scaffold	GCA_900167005	1121128.3	2585428076	1121128	69.9

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16766	Butyricicoccus pullicaecorum strain 25-3 16S ribosomal RNA gene, partial sequence	EU410376	1482		501571

GC content

@ref	GC-content (mol%)	Method
16766	54.5	high performance liquid chromatography (HPLC)
32584	54.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: IMG-taxon 2585428076 annotated assembly for Butyricicoccus pullicaecorum DSM 23266
NCBI: GCA_900167005

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	97.32	no
125439	gram_stain	BacteriaNetⓘ	negative	42.43	no
125439	motility	BacteriaNetⓘ	no	68.13	no
125439	spore_formation	BacteriaNetⓘ	no	89.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Butyricicoccus pullicaecorum DSM 23266 DSM 23266
NCBI: GCA_900167005.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	70.33	no
125438	anaerobic	anaerobicⓘ	yes	88.47	yes
125438	aerobic	aerobicⓘ	no	97.67	no
125438	spore-forming	spore-formingⓘ	no	52.00	no
125438	thermophilic	thermophileⓘ	no	89.77	yes
125438	flagellated	motile2+ⓘ	no	84.16	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	The combination of Butyricicoccus pullicaecorum and 3-hydroxyanthranilic acid prevents postmenopausal osteoporosis by modulating gut microbiota and Th17/Treg.	Zhu F, Liu H, Cao Y, Dai B, Wu H, Li W.	Eur J Nutr	10.1007/s00394-024-03400-3	2024
	Evolutionary Insights Into Microbiota Transplantation in Inflammatory Bowel Disease.	Wang X, Zhao J, Feng Y, Feng Z, Ye Y, Liu L, Kang G, Cao X.	Front Cell Infect Microbiol	10.3389/fcimb.2022.916543	2022
Metabolism	Butyrate-producing bacteria supplemented in vitro to Crohn's disease patient microbiota increased butyrate production and enhanced intestinal epithelial barrier integrity.	Geirnaert A, Calatayud M, Grootaert C, Laukens D, Devriese S, Smagghe G, De Vos M, Boon N, Van de Wiele T.	Sci Rep	10.1038/s41598-017-11734-8	2017
	Butyrate Producers as Potential Next-Generation Probiotics: Safety Assessment of the Administration of Butyricicoccus pullicaecorum to Healthy Volunteers.	Boesmans L, Valles-Colomer M, Wang J, Eeckhaut V, Falony G, Ducatelle R, Van Immerseel F, Raes J, Verbeke K	mSystems	10.1128/mSystems.00094-18	2018
Metabolism	Inulin-type fructan degradation capacity of Clostridium cluster IV and XIVa butyrate-producing colon bacteria and their associated metabolic outcomes.	Moens F, De Vuyst L	Benef Microbes	10.3920/BM2016.0142	2017
	The Probiotic Butyricicoccus pullicaecorum Reduces Feed Conversion and Protects from Potentially Harmful Intestinal Microorganisms and Necrotic Enteritis in Broilers.	Eeckhaut V, Wang J, Van Parys A, Haesebrouck F, Joossens M, Falony G, Raes J, Ducatelle R, Van Immerseel F	Front Microbiol	10.3389/fmicb.2016.01416	2016
Metabolism	Interindividual differences in response to treatment with butyrate-producing Butyricicoccus pullicaecorum 25-3T studied in an in vitro gut model.	Geirnaert A, Wang J, Tinck M, Steyaert A, Van den Abbeele P, Eeckhaut V, Vilchez-Vargas R, Falony G, Laukens D, De Vos M, Van Immerseel F, Raes J, Boon N, Van de Wiele T	FEMS Microbiol Ecol	10.1093/femsec/fiv054	2015
Genetics	Safety assessment of the butyrate-producing Butyricicoccus pullicaecorum strain 25-3(T), a potential probiotic for patients with inflammatory bowel disease, based on oral toxicity tests and whole genome sequencing.	Steppe M, Van Nieuwerburgh F, Vercauteren G, Boyen F, Eeckhaut V, Deforce D, Haesebrouck F, Ducatelle R, Van Immerseel F	Food Chem Toxicol	10.1016/j.fct.2014.06.024	2014
Phylogeny	Agathobaculum butyriciproducens gen. nov. sp. nov., a strict anaerobic, butyrate-producing gut bacterium isolated from human faeces and reclassification of Eubacterium desmolans as Agathobaculum desmolans comb. nov.	Ahn S, Jin TE, Chang DH, Rhee MS, Kim HJ, Lee SJ, Park DS, Kim BC	Int J Syst Evol Microbiol	10.1099/ijsem.0.001195	2016
Phylogeny	Butyricicoccus pullicaecorum gen. nov., sp. nov., an anaerobic, butyrate-producing bacterium isolated from the caecal content of a broiler chicken.	Eeckhaut V, Van Immerseel F, Teirlynck E, Pasmans F, Fievez V, Snauwaert C, Haesebrouck F, Ducatelle R, Louis P, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.65730-0	2008

References

#16766	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23266
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28799	IJSEM 2799 2008 ( DOI 10.1099/ijs.0.65730-0 , PubMed 19060061 )
#32584	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28799
#60513	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55265
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Butyricicoccus pullicaecorum (DSM 23266, CCUG 55265, LMG 24109)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900167005

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media