Clostridium hydrogeniformans (DSM 21757, NRRL B-51348, BL-20)

Name: Clostridium hydrogeniformans (DSM 21757, NRRL B-51348, BL-20)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2856

Type strain

Strain Designation BL-20

Culture col. no. DSM 21757 NRRL B-51348 BL-20

NCBI tax ID(s) 1121319 349933

Special Collections DSMZ Literature strains

History <- W. M. Moe, Louisiana State Univ.; BL-20 <- H.-S. Bae

Links

Clostridium hydrogeniformans BL-20 is an anaerobe, spore-forming, mesophilic prokaryote that was isolated from contaminated groundwater.

spore-forming Gram-positive motile rod-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 407505

DSM 21757

@ref 20215

Last LPSN update 2025-12-16 09:47:24

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium hydrogeniformans

Full scientific name Clostridium hydrogeniformans Bowman et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
29438	positive	3.75 µm	0.9 µm	rod-shaped

Pigmentation

29438

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
15920	PY + X MEDIUM (DSMZ Medium 104b)		Medium recipe at MediaDive	Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15920	positive	growth	35	mesophilic
29438	positive	growth	15-40
29438	positive	optimum	35	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
29438	positive	optimum	7.9
29438	positive	growth	05-10	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
15920	anaerobe
29438	facultative anaerobe
125439	anaerobe	99.7

Spore formation

29438

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29438	NaCl	positive	growth	<5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29438	4853 ChEBI	esculin	+	hydrolysis
29438	17814 ChEBI	salicin	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
29438	16136 ChEBI	hydrogen sulfide

Enzymes

@ref	Value	Activity
29438	gelatinase	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Contamination	-
#Environmental	#Aquatic	#Groundwater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15920	contaminated groundwater	10 miles north of Baton Rouge	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence DQ196623 (>99% sequence identity) for Clostridium hydrogeniformans subclade from Microbeatlas

Aquatic Samples	916
Soil Samples	1225
Animal Samples	374
Plant Samples	100
Total Samples	2615

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15920	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM68670v1 assembly for Clostridium hydrogeniformans DSM 21757	scaffold	GCA_000686705	1121319.3	2563366730	1121319	71.53

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15920	Clostridium hydrogeniformans strain BL-20 16S ribosomal RNA gene, partial sequence	DQ196623	1447		1121319

GC content

@ref	GC-content (mol%)	Method
15920	30.4	high performance liquid chromatography (HPLC)
29438	30.4

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Clostridium hydrogeniformans DSM 21757
NCBI: GCA_000686705

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	86.10	no
125439	motility	BacteriaNetⓘ	yes	83.10	no
125439	gram_stain	BacteriaNetⓘ	positive	78.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Clostridium hydrogeniformans DSM 21757 DSM 21757
NCBI: GCA_000686705.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	71.97	no
125438	anaerobic	anaerobicⓘ	yes	89.58	no
125438	aerobic	aerobicⓘ	no	98.56	yes
125438	spore-forming	spore-formingⓘ	yes	79.78	yes
125438	thermophilic	thermophileⓘ	no	94.73	no
125438	flagellated	motile2+ⓘ	yes	80.09	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Clostridium hydrogeniformans sp. nov. and Clostridium cavendishii sp. nov., hydrogen-producing bacteria from chlorinated solvent-contaminated groundwater.	Bowman KS, Dupre RE, Rainey FA, Moe WM	Int J Syst Evol Microbiol	10.1099/ijs.0.013169-0	2009

References

#15920	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21757
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25844	IJSEM 358 2010 ( DOI 10.1099/ijs.0.013169-0 , PubMed 19651721 )
#29438	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25844
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Clostridium hydrogeniformans (DSM 21757, NRRL B-51348, BL-20)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000686705

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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