Name: Enterocloster bolteae JCM 12243
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2821

Type strain:

Species: Enterocloster bolteae

Culture col. no.: JCM 12243, DSM 15670, ATCC BAA-613, CCUG 46953, WAL 16351, CIP 109439, VTT E-052776, AIP 10225

Strain history: CIP <- 2007, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10225 <- DSMZ <- ATCC <- S.M. Finegold: strain WAL 16351

NCBI tax ID(s): 208479 (species)

SI-ID 110852

CCUG 46953, ATCC BAA-613, DSM 15670, JCM 12243, VTT E-052776, CIP 109439

Other strains from Enterocloster bolteae

E. bolteae MT10-315-CC-8.2, DSM 29485
E. bolteae 90A5, CIP 110254

Section

Enterocloster bolteae JCM 12243 is an anaerobe, mesophilic, Gram-positive human pathogen that was isolated from stool specimens of children.

Gram-positive
rod-shaped
anaerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Lachnospiraceae*
	Genus	*Enterocloster*
	Species	**Enterocloster bolteae**
	Full Scientific Name (LPSN)	Enterocloster bolteae (Song et al. 2003) Haas and Blanchard 2020

	Synonym	"Lachnoclostridium* bolteae"*
	Synonym	Clostridium bolteae

Information on morphological and physiological properties Morphology

[Ref.: #116836]	Gram stain	positive

[Ref.: #116836]	Cell shape	rod-shaped

[Ref.: #116836]	Motility	no

[Ref.: #57419]

Incubation period

1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6024]

Culture medium

PY + X MEDIUM (DSMZ Medium 104b)

[Ref.: #6024]

Culture medium growth

[Ref.: #6024]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6024]

Culture medium composition

expand / minimize

[Ref.: #39709]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #39709]

Culture medium growth

[Ref.: #39709]

Culture medium composition

expand / minimize

[Ref.: #116836]

Culture medium

CIP Medium 20

[Ref.: #116836]

Culture medium growth

[Ref.: #116836]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6024]	growth	37 °C
[Ref.: #39709]	growth	37 °C
[Ref.: #57419]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #6024]	Temperature range	mesophilic
[Ref.: #39709]	Temperature range	mesophilic
[Ref.: #57419]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6024]	Oxygen tolerance	anaerobe
[Ref.: #57419]	Oxygen tolerance	anaerobe
[Ref.: #116836]	Oxygen tolerance	anaerobe

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116836]		nitrate ChEBI	+	reduction
[Ref.: #116836]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #116836]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116836]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116836]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116836]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116836]	11864	-	+	-	-	-	-	-	-	-	-	+	-	+	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6024]	Sample type/isolated from	stool specimens of children

[Ref.: #57419]	Sample type/isolated from	Human feces,child with late-onset autism
[Ref.: #57419]	Geographic location (country and/or sea, region)	Los Angeles
[Ref.: #57419]	Country	USA
[Ref.: #57419]	Country ISO 3 Code	USA
[Ref.: #57419]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Stool specimens of children with late-onset autism

[Ref.: #116836]	Sample type/isolated from	Human, Feces
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Infection	#Patient	#Specimen
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Child

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ508452 (>99% sequence identity) for Enterocloster bolteae from Microbeatlas

Aquatic Samples	1298
Soil Samples	424
Animal Samples	89539
Plant Samples	306
Total Samples	91567

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6024]	Pathogenicity (human)	yes, in single cases
[Ref.: #6024]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116836]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6024]	Clostridium boltei partial 16S rRNA gene, strain 16351	AJ508452	1498	ENA	208479 tax ID
[Ref.: #67770]	Clostridium bolteae gene for 16S ribosomal RNA, partial sequence, strain: JCM 12243	AB649278	1390	ENA	208479 tax ID
[Ref.: #20218]	Clostridium bolteae strain DSM 15670 16S ribosomal RNA gene, partial sequence	HM245933	846	ENA	208479 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Enterocloster bolteae ATCC BAA-613	GCA_000154365	scaffold	GenBank	411902 tax ID

[Ref.: #6024]	GC-content	49.0 mol%	sequence analysis
[Ref.: #67770]	GC-content	50.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterocloster bolteae ATCC BAA-613 ATCC BAA-613 NCBI: GCA_000154365.1
[Ref.: #69480]	motile	yes	76.782	no
[Ref.: #69480]	flagellated	no	64.312	no
[Ref.: #69480]	gram-positive	yes	77.863	no
[Ref.: #69480]	anaerobic	yes	98.871	yes
[Ref.: #69480]	aerobic	no	97.594	yes
[Ref.: #69480]	halophile	no	89.55	no
[Ref.: #69480]	spore-forming	yes	72.774	no
[Ref.: #69480]	thermophile	no	97.812	yes
[Ref.: #69480]	glucose-util	yes	92.422	no
[Ref.: #69480]	glucose-ferment	yes	74.363	no

Availability in culture collections External links

[Ref.: #6024]

Culture collection no.

JCM 12243, DSM 15670, ATCC BAA-613, CCUG 46953, WAL 16351, CIP 109439, VTT E-052776, AIP 10225

[Ref.: #72345]

SI-ID 110852

Literature:

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	ROESY and (13)C NMR to distinguish between D- and L-rhamnose in the alpha-D-Manp-(1 --> 4)-beta-Rhap-(1 --> 3) repeating motif.	Davidson J, Gauthier-Signore C, Bishop KP, Wicks C, Monteiro MA, Roy PN, Auzanneau FI	Org Biomol Chem	10.1039/d2ob00131d	2022	*
Phylogeny	Fusicatenibacter saccharivorans gen. nov., sp. nov., isolated from human faeces.	Takada T, Kurakawa T, Tsuji H, Nomoto K	Int J Syst Evol Microbiol	10.1099/ijs.0.045823-0	2013	*
Phylogeny	Clostridium bolteae sp. nov., isolated from human sources.	Song Y, Liu C, Molitoris DR, Tomzynski TJ, Lawson PA, Collins MD, Finegold SM	Syst Appl Microbiol	10.1078/072320203322337353	2003	*

References

#6024

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15670

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39709 ; Curators of the CIP;
#57419 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46953
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#72345

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID110852.1 )

#116836 Collection of Institut Pasteur ; Curators of the CIP; CIP 109439
* These data were automatically processed and therefore are not curated

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Enterocloster bolteae ATCC BAA-613 ATCC BAA-613

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