"Clostridium tunisiense" (DSM 15206, CIP 107666, E1)

BacDive ID 2814

Type strain

Strain Designation E1, E1T

Culture col. no. DSM 15206 CIP 107666 E1 E1T

NCBI tax ID(s) 1233099 219748

Special Collections DSMZ CIP

History <- M.-L. Fardeau, Universités de Provence et de la Méditerranée, ESIL, Marseille, France; E1 <- O. Ben Dhia Thabet {2001} 2002, M.L. Fardeau, IRD, Marseille, France: strain E1T CIP <- 2002, M.L. Fardeau, IRD, Marseille, France: strain E1T

Links

"Clostridium tunisiense" E1 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from anaerobic mud of an olive mill wastewater basin.

Gram-positive rod-shaped anaerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 97660

DSM 15206,CIP 107666

@ref 20215

Last LPSN update 2026-02-09 11:18:21

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species "Clostridium tunisiense"

Full scientific name Clostridium tunisiense Thabet et al. 2004

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
115970	positive	rod-shaped

Colony morphology

115970

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5757	ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503)	Medium recipe at MediaDive	Name: ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503; with strain-specific modifications) Composition: Yeast extract 1.99402 g/l Trypticase peptone 1.99402 g/l Na2CO3 1.49551 g/l NaCl 0.997008 g/l KCl 0.498505 g/l MgCl2 x 6 H2O 0.398803 g/l Na2S x 9 H2O 0.299103 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149552 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Calcium pantothenate 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water
40477	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
115970	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5757	positive	growth	37
40477	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
5757	anaerobe
115970	anaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
115970	17057 ChEBI	cellobiose	-	degradation
115970	15824 ChEBI	D-fructose	+	degradation
115970	17634 ChEBI	D-glucose	-	degradation
115970	65327 ChEBI	D-xylose	-	degradation
115970	4853 ChEBI	esculin	+	hydrolysis
115970	17716 ChEBI	lactose	-	degradation
115970	17306 ChEBI	maltose	+	degradation
115970	17632 ChEBI	nitrate	+	reduction
115970	17632 ChEBI	nitrate	+	respiration
115970	16301 ChEBI	nitrite	+	reduction
115970	17814 ChEBI	salicin	-	degradation
115970	17992 ChEBI	sucrose	-	degradation

Antibiotic resistance

@ref	ChEBI	Metabolite	Is sensitive	Is resistant
115970	6909	metronidazole

Metabolite production

@ref	Chebi-ID	Metabolite	Production
115970	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
115970	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
115970	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
115970	caseinase	+	3.4.21.50
115970	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
115970	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
115970	gelatinase	+
115970	lecithinase	+
68382	leucine arylamidase	-	3.4.11.1	from API zym
115970	lipase	+
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
115970	oxidase	-
115970	protease	+
68382	trypsin	+	3.4.21.4	from API zym
115970	tween esterase	-
115970	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
115970	-	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	-
#Engineered	#Waste	#Industrial wastewater
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
5757	anaerobic mud of an olive mill wastewater basin	Sfax	Tunisia	TUN	Africa
115970	Environment, Anaerobic mud of an olive mill waste water basin		Tunisia	TUN	Africa	2001

Taxonmaps

69479

Global distribution of 16S sequence AY187622 (>99% sequence identity) for Clostridium from Microbeatlas

Aquatic Samples	562
Soil Samples	602
Animal Samples	456
Plant Samples	96
Total Samples	1716

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
115970	2	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5757	Clostridium tunisiense 16S ribosomal RNA gene, partial sequence	AY187622	1475		1233099

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Microbial Composition Dynamics in Peloids Used for Spa Procedures in Lithuania: Pilot Study.	Katarzyte M, Rapoliene L, Kalvaitiene G, Picazo-Espinosa R.	Int J Environ Res Public Health	10.3390/ijerph21030335	2024
Genetics	Genome sequence of Clostridium tunisiense TJ, isolated from drain sediment from a pesticide factory.	Sun L, Wang Y, Yu C, Zhao Y, Gan Y.	J Bacteriol	10.1128/jb.01873-12	2012
Metabolism	Carbon Amendments Alter Microbial Community Structure and Net Mercury Methylation Potential in Sediments.	Christensen GA, Somenahally AC, Moberly JG, Miller CM, King AJ, Gilmour CC, Brown SD, Podar M, Brandt CC, Brooks SC, Palumbo AV, Wall JD, Elias DA.	Appl Environ Microbiol	10.1128/aem.01049-17	2018
Metabolism	Protein- and RNA-Enhanced Fermentation by Gut Microbiota of the Earthworm Lumbricus terrestris.	Zeibich L, Schmidt O, Drake HL.	Appl Environ Microbiol	10.1128/aem.00657-18	2018
Phylogeny	Analysis of the unexplored features of rrs (16S rDNA) of the Genus Clostridium.	Kalia VC, Mukherjee T, Bhushan A, Joshi J, Shankar P, Huma N.	BMC Genomics	10.1186/1471-2164-12-18	2011
Phylogeny	Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono-genomics description.	Bilen M, Mbogning Fonkou MD, Nguyen TT, Richez M, Daoud Z, Fournier PE, Raoult D, Cadoret F.	Microbiologyopen	10.1002/mbo3.735	2019
Phylogeny	Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse.	Thabet OB, Fardeau ML, Joulian C, Thomas P, Hamdi M, Garcia JL, Ollivier B	Anaerobe	10.1016/j.anaerobe.2004.04.002	2004

References

#5757	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15206
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#40477	; Curators of the CIP;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#115970	Collection of Institut Pasteur ; Curators of the CIP; CIP 107666
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

"Clostridium tunisiense" (DSM 15206, CIP 107666, E1)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

Help

Social Media