Name: Clostridium ljungdahlii PETC
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2802

Type strain:

Species: Clostridium ljungdahlii

Strain Designation: PETC

Culture col. no.: DSM 13528, ATCC 55383

Strain history: <- ATCC <- S. Barik, University of Arkansas Medical Sciences, Little Rock, USA; PETC

NCBI tax ID(s): 748727 (strain), 1538 (species)

SI-ID 43583

ATCC 55383, DSM 13528

Section

Clostridium ljungdahlii PETC is an anaerobe, spore-forming, mesophilic bacterium that was isolated from chicken yard waste.

motile
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium ljungdahlii**
	Full Scientific Name (LPSN)	Clostridium ljungdahlii Tanner et al. 1993

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %99.999

[Ref.: #69480]	Motility	yes Confidence in %91.786

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5077]

Culture medium

CLOSTRIDIUM LJUNGDAHLII MEDIUM (DSMZ Medium 879)

[Ref.: #5077]

Culture medium growth

[Ref.: #5077]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5077]

Culture medium composition

expand / minimize

[Ref.: #5077]

Culture medium

CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)

[Ref.: #5077]

Culture medium growth

[Ref.: #5077]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5077]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5077]			growth	37 °C

[Ref.: #5077]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5077]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.704

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5077]	Sample type/isolated from	chicken yard waste
[Ref.: #5077]	Geographic location (country and/or sea, region)	Arkansas
[Ref.: #5077]	Country	USA
[Ref.: #5077]	Country ISO 3 Code	USA
[Ref.: #5077]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Livestock (Husbandry)
#Engineered	#Waste	#Solid waste
#Host	#Birds	#Chicken

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FR733688 (>99% sequence identity) for Clostridium from Microbeatlas

Aquatic Samples	1409
Soil Samples	2899
Animal Samples	13922
Plant Samples	372
Total Samples	18602

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5077]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5077]	Clostridium ljungdahlii partial 16S rRNA gene, type strain DSM13528T	FR733688	1499	ENA	748727 tax ID
[Ref.: #20218]	Clostridium ljungdahlii strain ATCC 55383 16S ribosomal RNA gene, partial sequence	GU139552	1471	ENA	1538 tax ID	*
[Ref.: #20218]	Clostridium ljungdahlii strain DSM 13528 16S ribosomal RNA gene, partial sequence	DQ911275	320	ENA	748727 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium ljungdahlii DSM 13528	GCA_000143685	complete	GenBank	748727 tax ID	*
[Ref.: #66792]	Clostridium ljungdahlii DSM 13528 PETC	GCA_001636855	scaffold	GenBank	748727 tax ID	*
[Ref.: #66792]	Clostridium ljungdahlii DSM 13528 strain PETC	748727.19	wgs	PATRIC	748727 tax ID	*
[Ref.: #66792]	Clostridium ljungdahlii strain DSM 13528	1538.8	complete	PATRIC	1538 tax ID	*
[Ref.: #66792]	Clostridium ljungdahlii PETC	2740892543	draft	JGI IMG/M	748727 tax ID	*
[Ref.: #66792]	Clostridium ljungdahlii PETC, DSM 13528	648028017	complete	JGI IMG/M	748727 tax ID	*

[Ref.: #5077]

GC-content

22-23 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium ljungdahlii DSM 13528 DSM 13528 NCBI: GCA_000143685.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium ljungdahlii DSM 13528 DSM 13528 NCBI: GCA_000143685.1
[Ref.: #69480]	motile	yes	85.587	no
[Ref.: #69480]	flagellated	no	60.812	no
[Ref.: #69480]	gram-positive	yes	61.287	no
[Ref.: #69480]	anaerobic	yes	98.669	no
[Ref.: #69480]	aerobic	no	98.08	yes
[Ref.: #69480]	halophile	no	92.038	no
[Ref.: #69480]	spore-forming	yes	93.048	no
[Ref.: #69480]	thermophile	no	98.342	yes
[Ref.: #69480]	glucose-util	yes	84.728	no
[Ref.: #69480]	glucose-ferment	yes	55.05	no

Availability in culture collections External links

[Ref.: #5077]

Culture collection no.

DSM 13528, ATCC 55383

[Ref.: #72328]

SI-ID 43583

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain.	Whitham JM, Schulte MJ, Bobay BG, Bruno-Barcena JM, Chinn MS, Flickinger MC, Pawlak JJ, Grunden AM	Appl Microbiol Biotechnol	10.1007/s00253-016-7978-6	2016	*
Phylogeny	Clostridium luticellarii sp. nov., isolated from a mud cellar used for producing strong aromatic liquors.	Wang Q, Wang CD, Li CH, Li JG, Chen Q, Li YZ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000641	2015	*
Enzymology	Characterization of an acetoin reductase/2,3-butanediol dehydrogenase from Clostridium ljungdahlii DSM 13528.	Tan Y, Liu ZY, Liu Z, Li FL	Enzyme Microb Technol	10.1016/j.enzmictec.2015.06.011	2015	*
Metabolism	Physiological response of Clostridium ljungdahlii DSM 13528 of ethanol production under different fermentation conditions.	Xie BT, Liu ZY, Tian L, Li FL, Chen XH	Bioresour Technol	10.1016/j.biortech.2014.11.101	2014	*
Metabolism	UVC-mutagenesis in acetogens: resistance to methanol, ethanol, acetone, or n-butanol in recombinants with tailored genomes as the step in engineering of commercial biocatalysts for continuous CO(2)/H(2) blend fermentations.	Kiriukhin M, Tyurin M, Gak E	World J Microbiol Biotechnol	10.1007/s11274-013-1579-7	2014	*
Metabolism	RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically.	Tan Y, Liu J, Chen X, Zheng H, Li F	Mol Biosyst	10.1039/c3mb70232d	2013	*
Enzymology	Characterization of two novel butanol dehydrogenases involved in butanol degradation in syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528.	Tan Y, Liu J, Liu Z, Li F	J Basic Microbiol	10.1002/jobm.201300046	2013	*
	Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia.	Brown SD, Nagaraju S, Utturkar S, De Tissera S, Segovia S, Mitchell W, Land ML, Dassanayake A, Kopke M	Biotechnol Biofuels	10.1186/1754-6834-7-40	2014	*
	Evaluation of Clostridium ljungdahlii DSM 13528 reference genes in gene expression studies by qRT-PCR.	Liu J, Tan Y, Yang X, Chen X, Li F	J Biosci Bioeng	10.1016/j.jbiosc.2013.04.011	2013	*

References

#5077

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13528

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72328

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43583.1 )

* These data were automatically processed and therefore are not curated

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Clostridium ljungdahlii DSM 13528 DSM 13528

Clostridium ljungdahlii DSM 13528 DSM 13528

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