Name: Clostridium pascui Cm19
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2783

Type strain:

Species: Clostridium pascui

Strain Designation: Cm19, Cm 19

Culture col. no.: DSM 10365, CIP 105172, JCM 11012

Strain history: CIP <- 1997, H. Hippe, Germany: strain Cm 19 <- N. Tosunoglu

NCBI tax ID(s): 46609 (species)

SI-ID 48820

CIP 105172, DSM 10365, JCM 11012

Other strains from Clostridium pascui

C. pascui Cm17, DSM 10364
C. pascui Cm20, DSM 10366
C. pascui Cm21, DSM 10367

Section

Clostridium pascui Cm19 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from soil, donkey pasture.

motile
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium pascui**
	Full Scientific Name (LPSN)	Clostridium pascui Wilde et al. 1997

Information on morphological and physiological properties Morphology

[Ref.: #121616]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #121616]	Cell shape	rod-shaped

[Ref.: #121616]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %91.303

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3918]

Culture medium

WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)

[Ref.: #3918]

Culture medium growth

[Ref.: #3918]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3918]

Culture medium composition

expand / minimize

[Ref.: #3918]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #3918]

Culture medium growth

[Ref.: #3918]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3918]

Culture medium composition

expand / minimize

[Ref.: #39214]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #39214]

Culture medium growth

[Ref.: #39214]

Culture medium composition

expand / minimize

[Ref.: #121616]

Culture medium

CIP Medium 20

[Ref.: #121616]

Culture medium growth

[Ref.: #121616]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #3918]			growth	37 °C
[Ref.: #39214]			growth	37 °C

[Ref.: #3918]	Temperature range	mesophilic
[Ref.: #39214]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3918]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.251

[Ref.: #121616]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121616]	cellobiose ChEBI	-	degradation
[Ref.: #121616]	D-fructose ChEBI	-	degradation
[Ref.: #121616]	D-glucose ChEBI	-	degradation
[Ref.: #121616]	D-xylose ChEBI	-	degradation
[Ref.: #121616]	esculin ChEBI	-	hydrolysis
[Ref.: #121616]	lactose ChEBI	-	degradation
[Ref.: #121616]	maltose ChEBI	-	degradation
[Ref.: #121616]	nitrate ChEBI	-	reduction
[Ref.: #121616]	nitrate ChEBI	+	respiration
[Ref.: #121616]	nitrite ChEBI	-	reduction
[Ref.: #121616]	salicin ChEBI	-	degradation
[Ref.: #121616]	sucrose ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121616]		indole ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121616]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121616]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #121616]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121616]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121616]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121616]	1037	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121616]	8505	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3918]	Sample type/isolated from	soil, donkey pasture
[Ref.: #3918]	Country	Pakistan
[Ref.: #3918]	Country ISO 3 Code	PAK
[Ref.: #3918]	Continent	Asia

[Ref.: #121616]	Sample type/isolated from	Environment, Donkey, pasture soil
[Ref.: #121616]	Country	Pakistan
[Ref.: #121616]	Country ISO 3 Code	PAK
[Ref.: #121616]	Continent	Asia
[Ref.: #121616]	Isolation date	1981
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Grassland
#Environmental	#Terrestrial	#Soil
#Host	#Mammals	#Equidae (Horse)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence NR_026322 (>99% sequence identity) for Clostridium pascui from Microbeatlas

Aquatic Samples	543
Soil Samples	839
Animal Samples	469
Plant Samples	56
Total Samples	1907

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3918]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121616]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3918]	Clostridium pascui strain DSM 10365 16S ribosomal RNA, partial sequence	NR_026322	1471	GenBank	46609 tax ID
[Ref.: #20218]	C.pascui 16S rRNA gene	X96736	1471	ENA	46609 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium pascui DSM 10365	GCA_016908015	contig	GenBank	46609 tax ID	*
[Ref.: #66792]	Clostridium pascui strain DSM 10365	46609.3	wgs	PATRIC	46609 tax ID	*
[Ref.: #66792]	Clostridium pascui DSM 10365	2901044109	draft	JGI IMG/M	46609 tax ID	*

[Ref.: #3918]

GC-content

27 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium pascui strain DSM 10365 PATRIC: 46609.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium pascui strain DSM 10365 PATRIC: 46609.3
[Ref.: #69480]	gram-positive	no	52.644	no
[Ref.: #69480]	anaerobic	yes	98.892	yes
[Ref.: #69480]	halophile	no	94.092	no
[Ref.: #69480]	spore-forming	yes	94.428	no
[Ref.: #69480]	glucose-util	yes	86.208	no
[Ref.: #69480]	aerobic	no	97.432	no
[Ref.: #69480]	flagellated	yes	66.417	no
[Ref.: #69480]	thermophile	no	94.123	no
[Ref.: #69480]	motile	yes	84.984	no
[Ref.: #69480]	glucose-ferment	yes	52.814	no

Availability in culture collections External links

[Ref.: #3918]

Culture collection no.

DSM 10365, CIP 105172, JCM 11012

[Ref.: #72310]

SI-ID 48820

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Clostridium prolinivorans sp. nov., a thermophilic bacterium isolated from an anaerobic reactor degrading propionate.	Huang Y, Wei Z, Cong L, Qiu Z, Chen R, Deng Y, Zhang Y, Fan H, Ma S	Int J Syst Evol Microbiol	10.1099/ijsem.0.003523	2019	*
Phylogeny	Clostridium liquoris sp. nov., isolated from a fermentation pit used for the production of Chinese strong-flavoured liquor.	Yin Q, Tao Y, Zhu X, Zhou Y, He X, Cheng L, Huang Y, Li D	Int J Syst Evol Microbiol	10.1099/ijsem.0.000787	2015	*
Phylogeny	Gracilibacter thermotolerans gen. nov., sp. nov., an anaerobic, thermotolerant bacterium from a constructed wetland receiving acid sulfate water.	Lee YJ, Romanek CS, Mills GL, Davis RC, Whitman WB, Wiegel J	Int J Syst Evol Microbiol	10.1099/ijs.0.64040-0	2006	*
Phylogeny	Clostridium lundense sp. nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen.	Cirne DG, Delgado OD, Marichamy S, Mattiasson B	Int J Syst Evol Microbiol	10.1099/ijs.0.63730-0	2006	*
Metabolism	Clostridium pascui sp. nov., a new glutamate-fermenting sporeformer from a pasture in Pakistan.	Wilde E, Collins MD, Hippe H	Int J Syst Bacteriol	10.1099/00207713-47-1-164	1997	*

References

#3918

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10365

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39214 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72310

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID48820.1 )

#121616 Collection of Institut Pasteur ; Curators of the CIP; CIP 105172
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Clostridium pascui in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Clostridium pascui strain DSM 10365

Clostridium pascui strain DSM 10365

Change proposal

Change proposal