Name: Clostridium estertheticum subsp. estertheticum MT1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 2776

Type strain:

Species: Clostridium estertheticum subsp. estertheticum

Strain Designation: MT1

Culture col. no.: DSM 8809, ATCC 51377, NCIMB 12511, CIP 105093

Strain history: CIP <- 1997, NCIMB <- J.L. Smart: strain MT1

NCBI tax ID(s): 238834 (species), 1552 (subspecies)

SI-ID 42959

ATCC 51377, NCIMB 12511, DSM 8809, CIP 105093

Special Collections

DSMZ
CIP

Section

Clostridium estertheticum subsp. estertheticum MT1 is an anaerobe, spore-forming, psychrophilic bacterium that was isolated from vacuum packed beef.

Gram-positive
rod-shaped
spore-forming
anaerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium estertheticum subsp. estertheticum**
	Full Scientific Name (LPSN)	Clostridium estertheticum subsp. estertheticum (Collins et al. 1993) Spring et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #119135]	Gram stain	positive

[Ref.: #119135]	Cell shape	rod-shaped

[Ref.: #119135]	Motility	no

[Ref.: #3410]

Incubation period

>14 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3410]

Culture medium

RCM MEDIUM (N2/CO2) (DSMZ Medium 634c)

[Ref.: #3410]

Culture medium growth

[Ref.: #3410]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3410]

Culture medium composition

expand / minimize

[Ref.: #39195]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #39195]

Culture medium growth

[Ref.: #39195]

Culture medium composition

expand / minimize

[Ref.: #119135]

Culture medium

CIP Medium 20

[Ref.: #119135]

Culture medium growth

[Ref.: #119135]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #3410]			growth	10 °C
[Ref.: #39195]			growth	10 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3410]

Oxygen tolerance

anaerobe

[Ref.: #119135]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3410]	Sample type/isolated from	vacuum packed beef

[Ref.: #119135]	Sample type/isolated from	Food, Vacuum packed beef
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Bottled, canned, packed food
#Engineered	#Food production	#Meat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence S46734 (>99% sequence identity) for Clostridium from Microbeatlas

Aquatic Samples	5428
Soil Samples	9246
Animal Samples	1548
Plant Samples	2245
Total Samples	18467

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3410]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119135]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Clostridium estertheticum subsp. estertheticum strain DSM 8809 16S ribosomal RNA gene, partial sequence	DQ911250	328	GenBank ENA	1552 tax ID	*
[Ref.: #20218]	Clostridium estertheticum strain NCIBM 12511 16S ribosomal RNA gene, partial sequence	S46734	1427	GenBank ENA	238834 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium estertheticum subsp. estertheticum DSM 8809	GCA_001877035	complete	GenBank ENA	1552 tax ID	*
[Ref.: #66792]	Clostridium estertheticum subsp. estertheticum strain DSM 8809	1552.13	complete	PATRIC	1552 tax ID	*
[Ref.: #66792]	Clostridium estertheticum subsp. estertheticum strain DSM 8809	1552.14	plasmid	PATRIC	1552 tax ID	*
[Ref.: #66792]	Clostridium estertheticum estertheticum DSM 8809	2721755605	complete	JGI IMG/M	1552 tax ID	*

[Ref.: #3410]

GC-content

30.9 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium estertheticum subsp. estertheticum DSM 8809 NCBI: GCA_001877035.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium estertheticum subsp. estertheticum DSM 8809 NCBI: GCA_001877035.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	65.622	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	83.253	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	93.825	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	75.491	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.342	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	79.633	no

Availability in culture collections External links

[Ref.: #3410]

Culture collection no.

DSM 8809, ATCC 51377, NCIMB 12511, CIP 105093

[Ref.: #72306]

SI-ID 42959

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Clostridium estertheticum DSM 8809, a Microbe Identified in Spoiled Vacuum Packed Beef.	Yu Z, Gunn L, Brennan E, Reid R, Wall PG, Gaora PO, Hurley D, Bolton D, Fanning S	Front Microbiol	10.3389/fmicb.2016.01764	2016	*
Biotechnology	Effects of temperature and pH on the growth of bacteria isolated from blown packs of vacuum-packaged beef.	Yang X, Gill CO, Balamurugan S	J Food Prot	10.4315/0362-028x-72.11.2380	2009	*
Metabolism	Substrate utilization by Clostridium estertheticum cultivated in meat juice medium.	Yang X, Balamurugan S, Gill CO	Int J Food Microbiol	10.1016/j.ijfoodmicro.2008.10.024	2008	*
Phylogeny	Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Clostridium lacusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov.	Spring S, Merkhoffer B, Weiss N, Kroppenstedt RM, Hippe H, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.02554-0	2003	*
Phylogeny	Taxonomic studies on a psychrophilic Clostridium from vacuum-packed beef: description of Clostridium estertheticum sp. nov.	Collins MD, Rodrigues UM, Dainty RH, Edwards RA, Roberts TA	FEMS Microbiol Lett	10.1016/0378-1097(92)90410-p	1992	*

References

#3410

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8809

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39195 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72306

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42959.1 )

#119135 Collection of Institut Pasteur ; Curators of the CIP; CIP 105093
* These data were automatically processed and therefore are not curated

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Clostridium estertheticum subsp. estertheticum DSM 8809

Clostridium estertheticum subsp. estertheticum DSM 8809

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