Name: Anaerotignum neopropionicum X4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2765

Type strain:

Species: Anaerotignum neopropionicum

Strain Designation: X4

Culture col. no.: DSM 3847, KCTC 15564

Strain history: <- Stefan Spring, DSM <- JP Touzel, Institut National de la Recherche Agronomique, Villeneuve-d'Ascq, France, X4 <- E Samain, Centre National de la Recherche Scientifique, Grenoble, France

NCBI tax ID(s): 1121327 (strain), 36847 (species)

SI-ID 47766

DSM 3847

Section

Anaerotignum neopropionicum X4 is an anaerobe, chemoorganotroph, spore-forming bacterium that was isolated from anaerobic digester.

Gram-negative
motile
rod-shaped
spore-forming
anaerobe
chemoorganotroph
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Lachnospiraceae*
	Genus	*Anaerotignum*
	Species	**Anaerotignum neopropionicum**
	Full Scientific Name (LPSN)	Anaerotignum neopropionicum (Tholozan et al. 1995) Ueki et al. 2017

	Synonym	"Tyzzerella* neopropionica"*
	Synonym	Clostridium neopropionicum

Information on morphological and physiological properties Morphology

[Ref.: #43524]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #43524]	Cell length	2-5 µm

[Ref.: #43524]	Cell width	0.5-0.6 µm

[Ref.: #43524]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43524]	Motility	yes
[Ref.: #67771]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1477]

Culture medium

ANAEROTIGNUM NEOPROPIONICUM MEDIUM (DSMZ Medium 318b)

[Ref.: #1477]

Culture medium growth

[Ref.: #1477]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1477]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1477]			growth	30 °C
[Ref.: #67771]			growth	30 °C

[Ref.: #1477]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1477]	Oxygen tolerance	anaerobe
[Ref.: #43524]	Oxygen tolerance	obligate anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #43524]

Nutrition type

chemoorganotroph

[Ref.: #67771]	Type of spore	spore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #43524]	Ability of spore formation	yes

[Ref.: #1477]

Name of produced compound

propionate

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43524]	ethanol ChEBI	+	assimilation
[Ref.: #43524]	glucose ChEBI	-	assimilation
[Ref.: #43524]	L-alanine ChEBI	+	assimilation
[Ref.: #43524]	L-isoleucine ChEBI	+/-	assimilation
[Ref.: #43524]	L-leucine ChEBI	+/-	assimilation
[Ref.: #43524]	L-serine ChEBI	+	assimilation
[Ref.: #43524]	L-threonine ChEBI	+	assimilation
[Ref.: #43524]	L-valine ChEBI	+/-	assimilation
[Ref.: #43524]	xylose ChEBI	+	assimilation

	Metabolite	Production
[Ref.: #43524]	acetate ChEBI	yes
[Ref.: #43524]	butyrate ChEBI	yes
[Ref.: #43524]	isobutyrate ChEBI	yes
[Ref.: #43524]	isovalerate ChEBI	yes
[Ref.: #43524]	propionate ChEBI	yes

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	starch degradation	100	10 of 10
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	alanine metabolism	82.76	24 of 29
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	lipoate biosynthesis	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	chorismate metabolism	77.78	7 of 9
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	lactate fermentation	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	glutamate and glutamine metabolism	75	21 of 28
[Ref.: #66794]	toluene degradation	75	3 of 4
[Ref.: #66794]	pyrimidine metabolism	73.33	33 of 45
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	Entner Doudoroff pathway	70	7 of 10
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	purine metabolism	68.09	64 of 94
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	NAD metabolism	66.67	12 of 18
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	C4 and CAM-carbon fixation	62.5	5 of 8
[Ref.: #66794]	isoleucine metabolism	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	urea cycle	61.54	8 of 13
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	degradation of sugar acids	60	15 of 25
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	lipid A biosynthesis	55.56	5 of 9
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	tryptophan metabolism	55.26	21 of 38
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	pentose phosphate pathway	54.55	6 of 11
[Ref.: #66794]	methionine metabolism	53.85	14 of 26
[Ref.: #66794]	non-pathway related	52.63	20 of 38
[Ref.: #66794]	lysine metabolism	52.38	22 of 42
[Ref.: #66794]	polyamine pathway	52.17	12 of 23
[Ref.: #66794]	tetrahydrofolate metabolism	50	7 of 14
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	biotin biosynthesis	50	2 of 4
[Ref.: #66794]	citric acid cycle	50	7 of 14
[Ref.: #66794]	oxidative phosphorylation	49.45	45 of 91
[Ref.: #66794]	histidine metabolism	48.28	14 of 29
[Ref.: #66794]	aclacinomycin biosynthesis	42.86	3 of 7
[Ref.: #66794]	ubiquinone biosynthesis	42.86	3 of 7
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	factor 420 biosynthesis	40	2 of 5
[Ref.: #66794]	4-hydroxyphenylacetate degradation	40	4 of 10
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	isoprenoid biosynthesis	38.46	10 of 26
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
[Ref.: #66794]	metabolism of disaccharids	36.36	4 of 11
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	arachidonic acid metabolism	33.33	6 of 18
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	molybdenum cofactor biosynthesis	33.33	3 of 9
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	lipid metabolism	32.26	10 of 31
[Ref.: #66794]	degradation of pentoses	32.14	9 of 28
[Ref.: #66794]	androgen and estrogen metabolism	31.25	5 of 16
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	reductive acetyl coenzyme A pathway	28.57	2 of 7
[Ref.: #66794]	glutathione metabolism	28.57	4 of 14
[Ref.: #66794]	degradation of hexoses	27.78	5 of 18
[Ref.: #66794]	vitamin B6 metabolism	27.27	3 of 11
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	sulfate reduction	23.08	3 of 13
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
[Ref.: #66794]	allantoin degradation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1477]	Sample type/isolated from	anaerobic digester
[Ref.: #1477]	Geographic location (country and/or sea, region)	Lille
[Ref.: #1477]	Country	France
[Ref.: #1477]	Country ISO 3 Code	FRA
[Ref.: #1477]	Continent	Europe

[Ref.: #43524]	Sample type/isolated from	anoxic digester sludge

[Ref.: #67771]	Sample type/isolated from	From anoxic digestor sludge
[Ref.: #67771]	Geographic location (country and/or sea, region)	Lille
[Ref.: #67771]	Country	France
[Ref.: #67771]	Country ISO 3 Code	FRA
[Ref.: #67771]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Condition	#Anoxic (anaerobic)	-
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Sewage sludge

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1477]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1477]	Clostridium neopropionicum 16S rRNA gene, type strain DSM 3847T	X76746	1511	ENA	36847 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Anaerotignum neopropionicum X4	2651869904	draft	JGI IMG/M	36847 tax ID	*
[Ref.: #66792]	Anaerotignum neopropionicum DSM 3847	2728369180	draft	JGI IMG/M	36847 tax ID	*

[Ref.: #1477]	GC-content	34.5 mol%
[Ref.: #43524]	GC-content	34.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	34.0-35.0 mol%

Availability in culture collections External links

[Ref.: #1477]

Culture collection no.

DSM 3847, KCTC 15564

[Ref.: #72295]

SI-ID 47766

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Propionate Production from Carbon Monoxide by Synthetic Cocultures of Acetobacterium wieringae and Propionigenic Bacteria.	Moreira JPC, Diender M, Arantes AL, Boeren S, Stams AJM, Alves MM, Alves JI, Sousa DZ	Appl Environ Microbiol	10.1128/AEM.02839-20	2021	*
Phylogeny	Anaerotignum faecicola sp. nov., isolated from human faeces.	Choi SH, Kim JS, Park JE, Lee KC, Eom MK, Oh BS, Yu SY, Kang SW, Han KI, Suh MK, Lee DH, Yoon H, Kim BY, Lee JH, Lee JH, Lee JS, Park SH	J Microbiol	10.1007/s12275-019-9268-3	2019	*
Phylogeny	Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum.	Ueki A, Goto K, Ohtaki Y, Kaku N, Ueki K	Int J Syst Evol Microbiol	10.1099/ijsem.0.002268	2017	*
Genetics	Draft Genome Sequence of the Strict Anaerobe Clostridium neopropionicum X4 (DSM 3847T).	Beck MH, Poehlein A, Bengelsdorf FR, Schiel-Bengelsdorf B, Daniel R, Durre P	Genome Announc	10.1128/genomeA.00209-16	2016	*

References

#1477

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3847

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43524

Atsuko Ueki, Kazushi Goto, Yoshimi Ohtaki, Nobuo Kaku, Katsuji Ueki: Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum. IJSEM 67: 4146 - 4153 2017 ( DOI 10.1099/ijsem.0.002268 , PubMed 28905695 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#72295

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47766.1 )

* These data were automatically processed and therefore are not curated

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