Anaerotignum neopropionicum (DSM 3847, KCTC 15564, X4)

Name: Anaerotignum neopropionicum (DSM 3847, KCTC 15564, X4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2765

Type strain

Strain Designation X4

Culture col. no. DSM 3847 KCTC 15564 X4

NCBI tax ID(s) 1121327 36847

Special Collections DSMZ KCTC Literature strains

History <- J. P. Touzel, Institut National de la Recherche Agronomique, Villeneuve-d'Ascq, France; X4 <- E. Samain, Centre National de la Recherche Scientifique, Grenoble, France {1981} <- Stefan Spring, DSM <- JP Touzel, Institut National de la Recherche Agronomique, Villeneuve-d'Ascq, France, X4 <- E Samain, Centre National de la Recherche Scientifique, Grenoble, France

Links

Anaerotignum neopropionicum X4 is an anaerobe, chemoorganotroph, spore-forming bacterium that was isolated from anaerobic digester.

spore-forming Gram-negative motile rod-shaped anaerobe chemoorganotroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 47766

KCTC 15564,DSM 3847

@ref 20215

Last LPSN update 2026-02-09 11:18:21

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Lachnospiraceae

Genus Anaerotignum

Species Anaerotignum neopropionicum

Full scientific name Anaerotignum neopropionicum (Tholozan et al. 1995) Ueki et al. 2017

Synonyms (2)

"Tyzzerella neopropionica"
Clostridium neopropionicum

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
43524	negative	2-5 µm	0.5-0.6 µm	rod-shaped
67771				rod-shaped
67771	negative

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1477	ANAEROTIGNUM NEOPROPIONICUM MEDIUM (DSMZ Medium 318b)		Medium recipe at MediaDive	Name: ANAEROTIGNUM NEOPROPIONICUM MEDIUM (DSMZ Medium 318b) Composition: KHCO3 3.94867 g/l NH4Cl 0.987167 g/l Yeast extract 0.493583 g/l Trypticase peptone 0.493583 g/l Na2S x 9 H2O 0.29615 g/l KH2PO4 0.29615 g/l L-Cysteine HCl x H2O 0.29615 g/l Nitrilotriacetic acid 0.126357 g/l MgCl2 x 6 H2O 0.0987167 g/l FeCl2 x 4 H2O 0.00987167 g/l NaCl 0.00987167 g/l NiCl2 x 6 H2O 0.000987167 g/l ZnCl2 0.000987167 g/l CaCl2 x 2 H2O 0.000987167 g/l MnCl2 x 4 H2O 0.000987167 g/l Sodium resazurin 0.000493583 g/l Na2WO4 x 2 H2O 0.000394867 g/l CoCl2 x 6 H2O 0.00029615 g/l Na2MoO4 x 2 H2O 0.00029615 g/l Na2SeO3 x 5 H2O 0.00029615 g/l CuCl2 0.000197433 g/l H3BO3 9.87167e-05 g/l Pyridoxine hydrochloride 9.87167e-05 g/l Riboflavin 4.93583e-05 g/l Thiamine HCl 4.93583e-05 g/l Nicotinic acid 4.93583e-05 g/l (DL)-alpha-Lipoic acid 4.93583e-05 g/l Calcium D-(+)-pantothenate 4.93583e-05 g/l p-Aminobenzoic acid 4.93583e-05 g/l Biotin 1.97433e-05 g/l Folic acid 1.97433e-05 g/l Vitamin B12 9.87167e-07 g/l Ethanol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
1477	positive	growth	30
67771	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
1477	anaerobe
43524	obligate anaerobe
67771	anaerobe
125439	anaerobe	98

Nutrition type

43524

Typechemoorganotroph

Spore formation

@ref	Type of spore	Spore formation
43524
67771	spore

Compound production

1477

Compoundpropionate

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43524	16236 ChEBI	ethanol	+	assimilation
43524	17234 ChEBI	glucose	-	assimilation
43524	16977 ChEBI	L-alanine	+	assimilation
43524	17191 ChEBI	L-isoleucine	+/-	assimilation
43524	15603 ChEBI	L-leucine	+/-	assimilation
43524	17115 ChEBI	L-serine	+	assimilation
43524	16857 ChEBI	L-threonine	+	assimilation
43524	16414 ChEBI	L-valine	+/-	assimilation
43524	18222 ChEBI	xylose	+	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite
43524	30089 ChEBI	acetate
43524	17968 ChEBI	butyrate
43524	48944 ChEBI	isobutyrate
43524	48942 ChEBI	isovalerate
43524	17272 ChEBI	propionate

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	starch degradation	100	10 of 10
66794	folate polyglutamylation	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	cardiolipin biosynthesis	100	7 of 7
66794	threonine metabolism	90	9 of 10
66794	valine metabolism	88.89	8 of 9
66794	alanine metabolism	82.76	24 of 29
66794	peptidoglycan biosynthesis	80	12 of 15
66794	hydrogen production	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	lipoate biosynthesis	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	chorismate metabolism	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	phenylalanine metabolism	76.92	10 of 13
66794	butanoate fermentation	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	lactate fermentation	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	glutamate and glutamine metabolism	75	21 of 28
66794	toluene degradation	75	3 of 4
66794	pyrimidine metabolism	73.33	33 of 45
66794	flavin biosynthesis	73.33	11 of 15
66794	propanol degradation	71.43	5 of 7
66794	Entner Doudoroff pathway	70	7 of 10
66794	degradation of sugar alcohols	68.75	11 of 16
66794	purine metabolism	68.09	64 of 94
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	cyanate degradation	66.67	2 of 3
66794	NAD metabolism	66.67	12 of 18
66794	glycolysis	64.71	11 of 17
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	isoleucine metabolism	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	leucine metabolism	61.54	8 of 13
66794	urea cycle	61.54	8 of 13
66794	methylglyoxal degradation	60	3 of 5
66794	degradation of sugar acids	60	15 of 25
66794	glycine betaine biosynthesis	60	3 of 5
66794	lipid A biosynthesis	55.56	5 of 9
66794	cysteine metabolism	55.56	10 of 18
66794	d-mannose degradation	55.56	5 of 9
66794	tryptophan metabolism	55.26	21 of 38
66794	proline metabolism	54.55	6 of 11
66794	pentose phosphate pathway	54.55	6 of 11
66794	methionine metabolism	53.85	14 of 26
66794	non-pathway related	52.63	20 of 38
66794	lysine metabolism	52.38	22 of 42
66794	polyamine pathway	52.17	12 of 23
66794	tetrahydrofolate metabolism	50	7 of 14
66794	ethanol fermentation	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	arginine metabolism	50	12 of 24
66794	ketogluconate metabolism	50	4 of 8
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	biotin biosynthesis	50	2 of 4
66794	citric acid cycle	50	7 of 14
66794	oxidative phosphorylation	49.45	45 of 91
66794	histidine metabolism	48.28	14 of 29
66794	aclacinomycin biosynthesis	42.86	3 of 7
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	glycogen metabolism	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	factor 420 biosynthesis	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	isoprenoid biosynthesis	38.46	10 of 26
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
66794	metabolism of disaccharids	36.36	4 of 11
66794	tyrosine metabolism	35.71	5 of 14
66794	sphingosine metabolism	33.33	2 of 6
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	arachidonic acid metabolism	33.33	6 of 18
66794	IAA biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	molybdenum cofactor biosynthesis	33.33	3 of 9
66794	nitrate assimilation	33.33	3 of 9
66794	enterobactin biosynthesis	33.33	1 of 3
66794	lipid metabolism	32.26	10 of 31
66794	degradation of pentoses	32.14	9 of 28
66794	androgen and estrogen metabolism	31.25	5 of 16
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	coenzyme M biosynthesis	30	3 of 10
66794	myo-inositol biosynthesis	30	3 of 10
66794	reductive acetyl coenzyme A pathway	28.57	2 of 7
66794	glutathione metabolism	28.57	4 of 14
66794	degradation of hexoses	27.78	5 of 18
66794	vitamin B6 metabolism	27.27	3 of 11
66794	ascorbate metabolism	27.27	6 of 22
66794	cyclohexanol degradation	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	vitamin E metabolism	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	sulfate reduction	23.08	3 of 13
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	allantoin degradation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Condition	#Anoxic (anaerobic)	-
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Sewage sludge

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1477	anaerobic digester	Lille	France	FRA	Europe
43524	anoxic digester sludge
67771	From anoxic digestor sludge	Lille	France	FRA	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1477	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Anaerotignum neopropionicum X4	complete			2651869904	36847	88.67
124043	ASM157177v1 assembly for Anaerotignum neopropionicum DSM-3847	contig	GCA_001571775	36847.4	2728369180	36847	68.82

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
1477	Clostridium neopropionicum 16S rRNA gene, type strain DSM 3847T	X76746	1511		36847
124043	Anaerotignum neopropionicum DSM 3847 partial 16S rRNA gene	LR657300	734		1121327

GC content

@ref	GC-content (mol%)	Method
1477	34.5
43524	34.5	high performance liquid chromatography (HPLC)
67771	34.0-35.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Anaerotignum neopropionicum DSM 3847
IMG: 2728369180

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	79.50	no
125439	motility	BacteriaNetⓘ	yes	76.30	no
125439	gram_stain	BacteriaNetⓘ	variable	68.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	New Insights into the Physiology of the Propionate Producers Anaerotignum propionicum and Anaerotignum neopropionicum (Formerly Clostridium propionicum and Clostridium neopropionicum).	Baur T, Durre P.	Microorganisms	10.3390/microorganisms11030685	2023
	Enrichment of Anaerobic Syngas-Converting Communities and Isolation of a Novel Carboxydotrophic Acetobacterium wieringae Strain JM.	Arantes AL, Moreira JPC, Diender M, Parshina SN, Stams AJM, Alves MM, Alves JI, Sousa DZ.	Front Microbiol	10.3389/fmicb.2020.00058	2020
Pathogenicity	Production of propionate using metabolically engineered strains of Clostridium saccharoperbutylacetonicum.	Baur T, Wentzel A, Durre P.	Appl Microbiol Biotechnol	10.1007/s00253-022-12210-8	2022
	Rethinking Short-Chain Fatty Acids: A Closer Look at Propionate in Inflammation, Metabolism, and Mucosal Homeostasis	Facchin S, Calgaro M, Savarino E.	Cells		2025
Genetics	Synthetic Microbial Cocultivation for Targeted Production of Odd-Chain Carboxylates and Alcohols from Carbon Monoxide.	Parera Olm I, Benito-Vaquerizo S, Dubaere C, Martins Dos Santos VAP, Suarez-Diez M, Sousa DZ.	Environ Sci Technol	10.1021/acs.est.4c14794	2025
	Upgrading dilute ethanol to odd-chain carboxylic acids by a synthetic co-culture of Anaerotignum neopropionicum and Clostridium kluyveri.	Parera Olm I, Sousa DZ.	Biotechnol Biofuels Bioprod	10.1186/s13068-023-02336-w	2023
Metabolism	Genome-scale metabolic modelling enables deciphering ethanol metabolism via the acrylate pathway in the propionate-producer Anaerotignum neopropionicum.	Benito-Vaquerizo S, Parera Olm I, de Vroet T, Schaap PJ, Sousa DZ, Martins Dos Santos VAP, Suarez-Diez M.	Microb Cell Fact	10.1186/s12934-022-01841-1	2022
Genetics	Implementation of a Clostridium luticellarii genome-scale model for upgrading syngas fermentations.	Scott WT, Rockx S, Marien Q, Regueira A, Candry P, Ganigue R, Koehorst JJ, Schaap PJ.	Comput Struct Biotechnol J	10.1016/j.csbj.2025.01.013	2025
	Yeast mannan rich fraction positively influences microbiome uniformity, productivity associated taxa, and lay performance.	Leigh RJ, Corrigan A, Murphy RA, Taylor-Pickard J, Moran CA, Walsh F.	Anim Microbiome	10.1186/s42523-024-00295-7	2024
	Exploring the Potential of Oral Microbiome Biomarkers for Colorectal Cancer Diagnosis and Prognosis: A Systematic Review.	Negrut RL, Cote A, Maghiar AM.	Microorganisms	10.3390/microorganisms11061586	2023
	The oxidoreductase activity of Rnf balances redox cofactors during fermentation of glucose to propionate in Prevotella.	Zhang B, Lingga C, De Groot H, Hackmann TJ.	Sci Rep	10.1038/s41598-023-43282-9	2023
	Model-driven approach for the production of butyrate from CO₂/H₂ by a novel co-culture of C. autoethanogenum and C. beijerinckii.	Benito-Vaquerizo S, Nouse N, Schaap PJ, Hugenholtz J, Brul S, Lopez-Contreras AM, Martins Dos Santos VAP, Suarez-Diez M.	Front Microbiol	10.3389/fmicb.2022.1064013	2022
Genetics	Bacterial community structure alterations within the colorectal cancer gut microbiome.	Loftus M, Hassouneh SA, Yooseph S.	BMC Microbiol	10.1186/s12866-021-02153-x	2021
	The Multifunctional Sactipeptide Ruminococcin C1 Displays Potent Antibacterial Activity In Vivo as Well as Other Beneficial Properties for Human Health.	Roblin C, Chiumento S, Jacqueline C, Pinloche E, Nicoletti C, Olleik H, Courvoisier-Dezord E, Amouric A, Basset C, Dru L, Ollivier M, Bogey-Lambert A, Vidal N, Atta M, Maresca M, Devillard E, Duarte V, Perrier J, Lafond M.	Int J Mol Sci	10.3390/ijms22063253	2021
Phenotype	Genomic reconstruction of short-chain fatty acid production by the human gut microbiota.	Frolova MS, Suvorova IA, Iablokov SN, Petrov SN, Rodionov DA.	Front Mol Biosci	10.3389/fmolb.2022.949563	2022
Metabolism	Propionate Production from Carbon Monoxide by Synthetic Cocultures of Acetobacterium wieringae and Propionigenic Bacteria.	Moreira JPC, Diender M, Arantes AL, Boeren S, Stams AJM, Alves MM, Alves JI, Sousa DZ	Appl Environ Microbiol	10.1128/AEM.02839-20	2021
Genetics	Draft Genome Sequence of the Strict Anaerobe Clostridium neopropionicum X4 (DSM 3847T).	Beck MH, Poehlein A, Bengelsdorf FR, Schiel-Bengelsdorf B, Daniel R, Durre P	Genome Announc	10.1128/genomeA.00209-16	2016
Phylogeny	Anaerotignum faecicola sp. nov., isolated from human faeces.	Choi SH, Kim JS, Park JE, Lee KC, Eom MK, Oh BS, Yu SY, Kang SW, Han KI, Suh MK, Lee DH, Yoon H, Kim BY, Lee JH, Lee JH, Lee JS, Park SH	J Microbiol	10.1007/s12275-019-9268-3	2019
Phylogeny	Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum.	Ueki A, Goto K, Ohtaki Y, Kaku N, Ueki K	Int J Syst Evol Microbiol	10.1099/ijsem.0.002268	2017

References

#1477	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3847
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43524	Atsuko Ueki, Kazushi Goto, Yoshimi Ohtaki, Nobuo Kaku, Katsuji Ueki: Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum. IJSEM 67: 4146 - 4153 2017 ( DOI 10.1099/ijsem.0.002268 , PubMed 28905695 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Anaerotignum neopropionicum (DSM 3847, KCTC 15564, X4)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Compound production

Metabolite utilization

Metabolite production

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001571775

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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