Name: Ruminiclostridium cellulolyticum H10
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2760

Type strain:

Species: Ruminiclostridium cellulolyticum

Strain Designation: H10

Culture col. no.: DSM 5812, ATCC 35319, JCM 6584

Strain history: <- ATCC; ATCC 35319 <- E. Petitdemange, Université de Nancy, France; H10

NCBI tax ID(s): 1521 (species)

Section

Ruminiclostridium cellulolyticum H10 is an anaerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from decayed grass compost.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Oscillospiraceae*
	Genus	*Ruminiclostridium*
	Species	**Ruminiclostridium cellulolyticum**
	Full Scientific Name (PNU)	Ruminiclostridium cellulolyticum (Petitdemange et al. 1984) Zhang et al. 2018

Synonym

Clostridium cellulolyticum

Information on morphological and physiological properties Morphology

[Ref.: #43363]	Gram stain	positive
[Ref.: #43363]	Cell length	3-6 µm
[Ref.: #43363]	Cell width	0.6-1 µm
[Ref.: #43363]	Cell shape	rod-shaped
[Ref.: #43363]	Motility	yes
[Ref.: #43363]	Flagellum arrangement	peritrichous

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %90.199

[Ref.: #43363]	Colony size	0.5 mm
[Ref.: #43363]	Colony color	translucent and unpigmented
[Ref.: #43363]	Colony shape	circular
[Ref.: #43363]	Incubation period	5-6 days
[Ref.: #43363]	Cultivation medium used	Cellulose agar medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2323]

Culture medium

RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM (DSMZ Medium 520)

[Ref.: #2323]

Culture medium growth

[Ref.: #2323]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #2323]

Culture medium composition

expand / minimize

[Ref.: #43363]

Culture medium

Cellulose agar medium

[Ref.: #43363]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #2323]		growth	35 °C
[Ref.: #43363]	no	growth	50 °C
[Ref.: #43363]	no	growth	20 °C
[Ref.: #43363]		minimum	25 °C
[Ref.: #43363]		optimum	32-35 °C
[Ref.: #43363]		maximum	45 °C

[Ref.: #2323]	Temperature range	mesophilic
[Ref.: #43363]	Temperature range	thermophilic
[Ref.: #43363]	Temperature range	psychrophilic
[Ref.: #43363]	Temperature range	mesophilic
[Ref.: #43363]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #2323]	Oxygen tolerance	anaerobe
[Ref.: #43363]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.669

[Ref.: #43363]	Spore description	produce swollen cells, present in cultures on cellulose media 3 or more days old
[Ref.: #43363]	Type of spore	spore
[Ref.: #43363]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #2323]

Name of produced compound

sugar

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43363]	amygdalin ChEBI	-	growth
[Ref.: #43363]	arabinose ChEBI	+/-	growth
[Ref.: #43363]	casein	-	degradation
[Ref.: #43363]	cellobiose ChEBI	+/-	growth
[Ref.: #43363]	cellulose ChEBI	+/-	growth
[Ref.: #43363]	erythritol ChEBI	-	growth
[Ref.: #43363]	esculin ChEBI	+	hydrolysis
[Ref.: #43363]	fructose ChEBI	+/-	growth
[Ref.: #43363]	galactitol ChEBI	-	growth
[Ref.: #43363]	galactose ChEBI	+/-	growth
[Ref.: #43363]	gelatin ChEBI	-	hydrolysis
[Ref.: #43363]	glucose ChEBI	+/-	growth
[Ref.: #43363]	glycerol ChEBI	-	growth
[Ref.: #43363]	glycogen ChEBI	-	growth
[Ref.: #43363]	inulin ChEBI	-	growth
[Ref.: #43363]	lactate ChEBI	-	assimilation
[Ref.: #43363]	lactose ChEBI	-	growth
[Ref.: #43363]	maltose ChEBI	-	growth
[Ref.: #43363]	mannitol ChEBI	-	growth
[Ref.: #43363]	mannose ChEBI	+/-	growth
[Ref.: #43363]	melezitose ChEBI	-	growth
[Ref.: #43363]	milk	-	assimilation
[Ref.: #43363]	myo-inositol ChEBI	-	growth
[Ref.: #43363]	pyruvate ChEBI	-	assimilation
[Ref.: #43363]	raffinose ChEBI	-	growth
[Ref.: #43363]	rhamnose ChEBI	-	growth
[Ref.: #43363]	ribitol ChEBI	-	growth
[Ref.: #43363]	ribose ChEBI	+/-	growth
[Ref.: #43363]	salicin ChEBI	-	growth
[Ref.: #43363]	sorbitol ChEBI	-	growth
[Ref.: #43363]	sorbose ChEBI	-	growth
[Ref.: #43363]	starch ChEBI	-	hydrolysis
[Ref.: #43363]	sucrose ChEBI	-	growth
[Ref.: #43363]	thiosulfate ChEBI	-	reduction
[Ref.: #43363]	trehalose ChEBI	-	growth
[Ref.: #43363]	xylose ChEBI	+/-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #43363]		acetate ChEBI
[Ref.: #43363]		carbon dioxide ChEBI
[Ref.: #43363]		dihydrogen ChEBI
[Ref.: #43363]		DL-lactate ChEBI
[Ref.: #43363]		ethanol ChEBI
[Ref.: #43363]		formate ChEBI
[Ref.: #43363]		indole ChEBI
[Ref.: #43363]		nitrite ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #43363]	catalase	-	1.11.1.6
[Ref.: #43363]	lecithinase	-
[Ref.: #43363]	lipase	-
[Ref.: #43363]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2323]	Sample type/isolated from	decayed grass compost
[Ref.: #2323]	Country	France
[Ref.: #2323]	Country ISO 3 Code	FRA
[Ref.: #2323]	Continent	Europe

[Ref.: #43363]	Sample type/isolated from	isolated in autumn from decayed grass compost packed for 3 to 4 months
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Host	#Plants	#Decomposing plant
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2323]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	C.cellulolyticum gene for 16S ribosomal RNA	X71847	1642	ENA	394503 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ruminiclostridium cellulolyticum H10	GCA_000022065	complete	GenBank	394503 tax ID	*
[Ref.: #66792]	Clostridium cellulolyticum H10	394503.7	complete	PATRIC	394503 tax ID	*
[Ref.: #66792]	Ruminiclostridium cellulolyticum H10	643348528	complete	JGI IMG/M	394503 tax ID	*

[Ref.: #2323]	GC-content	41.0 mol%
[Ref.: #43363]	GC-content	41 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium cellulolyticum H10 PATRIC: 394503.7
[Ref.: #69480]	gram-positive	yes	100	yes
[Ref.: #69480]	spore-forming	yes	100	no
[Ref.: #69480]	anaerobic	yes	99.669	no
[Ref.: #69480]	thermophile	yes	51.753	no
[Ref.: #69480]	motile	yes	90.199	yes

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium cellulolyticum H10 PATRIC: 394503.7
[Ref.: #69481]	spore-forming	yes	87	no

Availability in culture collections External links

[Ref.: #2323]

Culture collection no.

DSM 5812, ATCC 35319, JCM 6584

[Ref.: #20218]

Associated Passport(s) in StrainInfo

159461, 110514, 110516

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Enzymology	Cloning, expression, and characterization of a D-psicose 3-epimerase from Clostridium cellulolyticum H10.	Mu W, Chu F, Xing Q, Yu S, Zhou L, Jiang B	J Agric Food Chem	10.1021/jf201356q	2011	*
Metabolism	Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia.	Desvaux M	FEMS Microbiol Rev	10.1016/j.femsre.2004.11.003	2004	*
Metabolism	Use of antisense RNA to modify the composition of cellulosomes produced by Clostridium cellulolyticum.	Perret S, Maamar H, Belaich JP, Tardif C	Mol Microbiol	10.1046/j.1365-2958.2003.03860.x	2004	*
Metabolism	Gene transfer to Clostridium cellulolyticum ATCC 35319.	Jennert KCB, Tardif C, Young DI, Young M	Microbiology (Reading)	10.1099/00221287-146-12-3071	2000	*
Metabolism	Spo0A directly controls the switch from acid to solvent production in solvent-forming clostridia.	Ravagnani A, Jennert KC, Steiner E, Grunberg R, Jefferies JR, Wilkinson SR, Young DI, Tidswell EC, Brown DP, Youngman P, Morris JG, Young M	Mol Microbiol	10.1046/j.1365-2958.2000.02071.x	2000	*
Metabolism	Studies of Clostridium cellulolyticum ATCC 35319 under dialysis and co-culture conditions.	Gehin A, Cailliez C, Petitdemange E, Benoit L	Lett Appl Microbiol	10.1111/j.1472-765x.1996.tb00067.x	1996	*
Metabolism	Interaction between the endoglucanase CelA and the scaffolding protein CipC of the Clostridium cellulolyticum cellulosome.	Pages S, Belaich A, Tardif C, Reverbel-Leroy C, Gaudin C, Belaich JP	J Bacteriol	10.1128/jb.178.8.2279-2286.1996	1996	*
Metabolism	The effects of tunicamycin on secretion, adhesion and activities of the cellulase complex of Clostridium cellulolyticum, ATCC 35319.	Gehin A, Petitdemange H	Res Microbiol	10.1016/0923-2508(96)80281-6	1995	*
Metabolism	Adhesion and growth rate of Clostridium cellulolyticum ATCC 35319 on crystalline cellulose.	Gelhaye E, Petitdemange H, Gay R	J Bacteriol	10.1128/jb.175.11.3452-3458.1993	1993	*
	Structure and regulation of the cellulose degradome in Clostridium cellulolyticum.	Xu C, Huang R, Teng L, Wang D, Hemme CL, Borovok I, He Q, Lamed R, Bayer EA, Zhou J, Xu J	Biotechnol Biofuels	10.1186/1754-6834-6-73	2013	*
	Colonization of Crystalline Cellulose by Clostridium cellulolyticum ATCC 35319.	Gelhaye E, Gehin A, Petitdemange H	Appl Environ Microbiol	10.1128/aem.59.9.3154-3156.1993	1993	*

References

#2323

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5812

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#43363

E. Petitdemange, F. Caillet, J. Giallo, C. Gaudin: Clostridium cellulolyticum sp. nov., a Cellulolytic, Mesophilic: Species from Decayed Grass. IJSEM 34: 155 - 159 1984 ( DOI 10.1099/00207713-34-2-155 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Clostridium cellulolyticum H10

Clostridium cellulolyticum H10

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