Name: Clostridium subterminale DSM 6970
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2720

Type strain:

Species: Clostridium subterminale

Culture col. no.: DSM 6970, ATCC 25774, CDC KA152, CCUG 21841, VPI 2023, JCM 1417, BCRC 14486, NCIMB 10746

Strain history: ATCC 25774 <-- L. DS. Smith VPI 2023 (CDC KA 152).

NCBI tax ID(s): 1550 (species)

SI-ID 38662

ATCC 25774, CCUG 21841, DSM 6970, JCM 1417, CCRC 14486, NCIMB 10746, BCRC 14486

Other strains from Clostridium subterminale

C. subterminale Christensen 517, 2778, DSM 758, ...
C. subterminale SB4, DSM 2636, ATCC 29748

Section

Clostridium subterminale DSM 6970 is an anaerobe, mesophilic bacterium of the family Clostridiaceae.

anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium subterminale**
	Full Scientific Name (LPSN)	Clostridium subterminale (Hall and Whitehead 1927) Spray 1948 (Approved Lists 1980)

Synonym

"Bacillus subterminalis"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2930]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #2930]

Culture medium growth

[Ref.: #2930]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2930]

Culture medium composition

expand / minimize

[Ref.: #2930]

Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

[Ref.: #2930]

Culture medium growth

[Ref.: #2930]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2930]

Culture medium composition

expand / minimize

[Ref.: #2930]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #2930]

Culture medium growth

[Ref.: #2930]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2930]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #2930]	growth	37 °C
[Ref.: #47506]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #2930]	Temperature range	mesophilic
[Ref.: #47506]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #2930]	Oxygen tolerance	anaerobe
[Ref.: #47506]	Oxygen tolerance	anaerobe

[Ref.: #2930]

Name of produced compound

methyl sulfides

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68367]	cellobiose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-glucose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-xylose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	esculin ChEBI	+	hydrolysis	* from API 20A
[Ref.: #68367]	gelatin ChEBI	+	hydrolysis	* from API 20A
[Ref.: #68367]	glycerol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-arabinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-rhamnose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	lactose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	maltose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	raffinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	salicin ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sucrose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	tryptophan ChEBI	-	energy source	* from API 20A
[Ref.: #68367]	urea ChEBI	-	hydrolysis	* from API 20A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68367]		indole ChEBI	no	* from API 20A

	Physiological tests	Metabolite	Indole test
[Ref.: #68367]		indole ChEBI	-	* from API 20A

	Enzyme	Enzyme activity	EC number
[Ref.: #68367]	beta-glucosidase	+	3.2.1.21	* from API 20A
[Ref.: #68367]	gelatinase	+		* from API 20A
[Ref.: #68367]	urease	-	3.5.1.5	* from API 20A

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #47506]

Fatty acid	Percentage	ECL
C16:0	29.6	16
C14:0	12.5	14
C18:2 ω6,9c/C18:0 ANTE	12.1	17.724
C17:1 ISO I/C16:0 DMA	8	16.481
C18:1 ω9c	7.1	17.769
C16:1 ω7c	4.7	15.819
C18:0	4	18
C16:1 ω9c	3.2	15.774
C16:1 ω7c DMA	3	16.288
C16:0 aldehyde	2.6	14.949
C18:1 ω6c	2.3	17.858
C18:1 ω9c DMA	2.1	18.226
C18:1 ω7c /12t/9t	1.9	17.824
C15:0 ISO	1.8	14.621
C16:1 ω9c DMA	1.6	16.242
C15:0 ANTEISO	1.4	14.711
C13:0 iso	1.1	12.612
unknown 18.177	1	18.177

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #2930]	2205	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence L37595 (>99% sequence identity) for Clostridium from Microbeatlas

Aquatic Samples	4474
Soil Samples	12801
Animal Samples	5452
Plant Samples	2582
Total Samples	25309

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2930]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2930]	Clostridium subterminale isolate DSM 6970 16S ribosomal RNA gene, partial sequence	AF241844	1504	ENA	1550 tax ID
[Ref.: #67770]		X68510	1516	ENA
[Ref.: #20218]	Clostridium subterminale strain ATCC 25774 16S ribosomal RNA gene, partial sequence	DQ911252	320	ENA	1550 tax ID	*
[Ref.: #20218]	Clostridium subterminale 16S ribosomal RNA (16S rRNA) gene	L37595	1448	ENA	1550 tax ID	*
[Ref.: #20218]	Clostridium subterminale 16S ribosomal RNA	M59106	1464	ENA	1550 tax ID	*
[Ref.: #20218]	Clostridium subterminale gene for 16S rRNA, partial sequence, strain: DSM 6970	AB294137	1452	ENA	1550 tax ID	*
[Ref.: #20218]	Clostridium subterminale gene for 16S ribosomal RNA, partial sequence, strain: JCM 1417	AB538430	1470	ENA	1550 tax ID	*
[Ref.: #20218]	C.subterminale rrn gene for 16S rRNA	X68451	1516	ENA	1550 tax ID	*

Availability in culture collections External links

[Ref.: #2930]

Culture collection no.

DSM 6970, ATCC 25774, CDC KA152, CCUG 21841, VPI 2023, JCM 1417, BCRC 14486, NCIMB 10746

[Ref.: #72248]

SI-ID 38662

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Clostridium culturomicium sp. nov. and Clostridium jeddahitimonense sp. nov., novel members of the Clostridium genus isolated from the stool of an obese Saudi Arabian.	Kieu HT, Garrigou N, Fadlane A, Brechard L, Armstrong N, Decloquement P, Yasir M, Azhar EI, Al-Masaudi SB, Lagier JC, Tidjani Alou M, Raoult D	Curr Microbiol	10.1007/s00284-021-02616-4	2021	*
Phylogeny	Clostridium punense sp. nov., an obligate anaerobe isolated from healthy human faeces.	Lanjekar VB, Marathe NP, Shouche YS, Ranade DR	Int J Syst Evol Microbiol	10.1099/ijsem.0.000644	2015	*
Phylogeny	Clostridium huakuii sp. nov., an anaerobic, acetogenic bacterium isolated from methanogenic consortia.	Ruan Z, Wang Y, Zhang C, Song J, Zhai Y, Zhuang Y, Wang H, Chen X, Li Y, Zhao B, Zhao B	Int J Syst Evol Microbiol	10.1099/ijs.0.062711-0	2014	*
Phylogeny	Clostridium sulfidigenes sp. nov., a mesophilic, proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from pond sediment.	Sallam A, Steinbuchel A	Int J Syst Evol Microbiol	10.1099/ijs.0.004986-0	2009	*
Phylogeny	Clostridium schirmacherense sp. nov., an obligately anaerobic, proteolytic, psychrophilic bacterium isolated from lake sediment of Schirmacher Oasis, Antarctica.	Alam SI, Dixit A, Reddy GSN, Dube S, Palit M, Shivaji S, Singh L	Int J Syst Evol Microbiol	10.1099/ijs.0.63808-0	2006	*
Phylogeny	Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor.	Hernandez-Eugenio G, Fardeau ML, Cayol JL, Patel BKC, Thomas P, Macarie H, Garcia JL, Ollivier B	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1461	2002	*

References

#2930

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6970

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#47506 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 21841
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68367

Automatically annotated from API 20A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72248

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID38662.1 )

* These data were automatically processed and therefore are not curated

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