Clostridium subterminale (DSM 6970, ATCC 25774, CDC KA152)

Name: Clostridium subterminale (DSM 6970, ATCC 25774, CDC KA152)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2720

Type strain

Culture col. no. DSM 6970 ATCC 25774 CDC KA152 CCUG 21841 VPI 2023 3 more

NCBI tax ID(s) 1550

Special Collections DSMZ CCUG JCM

History <- A. Rimbault; <- ATCC; ATCC 25774 <- L. DS. Smith; VPI 2023 ATCC 25774 <-- L. DS. Smith VPI 2023 (CDC KA 152).

Links

Clostridium subterminale DSM 6970 is an anaerobe, mesophilic prokaryote of the family Clostridiaceae.

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 38662

ATCC 25774,CCUG 21841,DSM 6970,JCM 1417,CCRC 14486,NCIMB 10746,BCRC 14486

@ref 20215

Last LPSN update 2026-02-09 11:18:20

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium subterminale

Full scientific name Clostridium subterminale (Hall and Whitehead 1927) Spray 1948 (Approved Lists 1980)

Synonyms (1)

"Bacillus subterminalis"

BacDive ID	Other strains from **Clostridium subterminale** (2)	Type strain
2718	C. subterminale Christensen 517, 2778, DSM 758, NCIB 9384
2719	C. subterminale SB4, DSM 2636, ATCC 29748

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2930	CHOPPED MEAT MEDIUM (DSMZ Medium 78)	Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
2930	FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)	Medium recipe at MediaDive	Name: FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a) Composition: Fastidious Anaerobe Basal Broth 35.4 g/l Distilled water
2930	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
2930	positive	growth	37	mesophilic
47506	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
2930	anaerobe
47506	anaerobe

Compound production

2930

Compoundmethyl sulfides

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68367	17057 ChEBI	cellobiose	-	builds acid from	from API 20A
68367	17634 ChEBI	D-glucose	-	builds acid from	from API 20A
68367	16899 ChEBI	D-mannitol	-	builds acid from	from API 20A
68367	16024 ChEBI	D-mannose	-	builds acid from	from API 20A
68367	65327 ChEBI	D-xylose	-	builds acid from	from API 20A
68367	4853 ChEBI	esculin	+	hydrolysis	from API 20A
68367	5291 ChEBI	gelatin	+	hydrolysis	from API 20A
68367	17754 ChEBI	glycerol	-	builds acid from	from API 20A
68367	30849 ChEBI	L-arabinose	-	builds acid from	from API 20A
68367	62345 ChEBI	L-rhamnose	-	builds acid from	from API 20A
68367	17716 ChEBI	lactose	-	builds acid from	from API 20A
68367	17306 ChEBI	maltose	-	builds acid from	from API 20A
68367	6731 ChEBI	melezitose	-	builds acid from	from API 20A
68367	16634 ChEBI	raffinose	-	builds acid from	from API 20A
68367	17814 ChEBI	salicin	-	builds acid from	from API 20A
68367	30911 ChEBI	sorbitol	-	builds acid from	from API 20A
68367	17992 ChEBI	sucrose	-	builds acid from	from API 20A
68367	27082 ChEBI	trehalose	-	builds acid from	from API 20A
68367	27897 ChEBI	tryptophan	-	energy source	from API 20A
68367	16199 ChEBI	urea	-	hydrolysis	from API 20A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68367	35581 ChEBI	indole		from API 20A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68367	35581 ChEBI	indole	-	from API 20A

Enzymes

@ref	Value	Activity	Ec
68367	beta-glucosidase	+	3.2.1.21	from API 20A
68367	gelatinase	+		from API 20A
68367	urease	-	3.5.1.5	from API 20A

Fatty acid profile

Metadata FA analysis

@ref

47506

Fatty acid	Percentage	ECL
C16:0	29.6	16
C14:0	12.5	14
C18:2 ω6,9c/C18:0 ANTE	12.1	17.724
C17:1 ISO I/C16:0 DMA	8	16.481
C18:1 ω9c	7.1	17.769
C16:1 ω7c	4.7	15.819
C18:0	4	18
C16:1 ω9c	3.2	15.774
C16:1 ω7c DMA	3	16.288
C16:0 aldehyde	2.6	14.949
C18:1 ω6c	2.3	17.858
C18:1 ω9c DMA	2.1	18.226
C18:1 ω7c /12t/9t	1.9	17.824
C15:0 ISO	1.8	14.621
C16:1 ω9c DMA	1.6	16.242
C15:0 ANTEISO	1.4	14.711
C13:0 iso	1.1	12.612
unknown 18.177	1	18.177

API 20A

@ref	IND	URE	GLU	MAN	LAC	SAC	MAL	SAL	XYL	ARA	GEL	ESC	GLY	CEL	MNE	MLZ	RAF	SOR	RHA	TRE	CAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
2930	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence L37595 (>99% sequence identity) for Clostridium from Microbeatlas

Aquatic Samples	4474
Soil Samples	12801
Animal Samples	5452
Plant Samples	2582
Total Samples	25309

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2930	2	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3952142v1 assembly for Clostridium subterminale JCM 1417	scaffold	GCA_039521425	1550	70.48

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Clostridium subterminale strain ATCC 25774 16S ribosomal RNA gene, partial sequence	DQ911252	320	1550
20218	Clostridium subterminale 16S ribosomal RNA (16S rRNA) gene	L37595	1448	1550
20218	Clostridium subterminale 16S ribosomal RNA	M59106	1464	1550
20218	Clostridium subterminale gene for 16S rRNA, partial sequence, strain: DSM 6970	AB294137	1452	1550
20218	Clostridium subterminale gene for 16S ribosomal RNA, partial sequence, strain: JCM 1417	AB538430	1470	1550
20218	C.subterminale rrn gene for 16S rRNA	X68451	1516	1550
2930	Clostridium subterminale isolate DSM 6970 16S ribosomal RNA gene, partial sequence	AF241844	1504	1550

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Culture-based and culture-independent approach for the study of the methanogens and obligate anaerobes from different landfill sites.	Prakash O, Dewala SR, Nimonkar Y, Patil SK, Chauhan A, Yadav A, Dhotre DP, Ranade DR.	Front Microbiol	10.3389/fmicb.2023.1273037	2023
Biotechnology	Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test.	Fach P, Popoff MR.	Appl Environ Microbiol	10.1128/aem.63.11.4232-4236.1997	1997
	Liquid chromatographic procedure for fermentation product analysis in the identification of anaerobic bacteria.	Ehrlich GG, Goerlitz DF, Bourell JH, Eisen GV, Godsy EM.	Appl Environ Microbiol	10.1128/aem.42.5.878-885.1981	1981
	Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C. difficile-associated disease.	DiPersio JR, Varga FJ, Conwell DL, Kraft JA, Kozak KJ, Willis DH.	J Clin Microbiol	10.1128/jcm.29.12.2724-2730.1991	1991
Phylogeny	Clostridium culturomicium sp. nov. and Clostridium jeddahitimonense sp. nov., novel members of the Clostridium genus isolated from the stool of an obese Saudi Arabian.	Kieu HT, Garrigou N, Fadlane A, Brechard L, Armstrong N, Decloquement P, Yasir M, Azhar EI, Al-Masaudi SB, Lagier JC, Tidjani Alou M, Raoult D	Curr Microbiol	10.1007/s00284-021-02616-4	2021
Phylogeny	Clostridium punense sp. nov., an obligate anaerobe isolated from healthy human faeces.	Lanjekar VB, Marathe NP, Shouche YS, Ranade DR	Int J Syst Evol Microbiol	10.1099/ijsem.0.000644	2015
Phylogeny	Clostridium huakuii sp. nov., an anaerobic, acetogenic bacterium isolated from methanogenic consortia.	Ruan Z, Wang Y, Zhang C, Song J, Zhai Y, Zhuang Y, Wang H, Chen X, Li Y, Zhao B, Zhao B	Int J Syst Evol Microbiol	10.1099/ijs.0.062711-0	2014
Phylogeny	Clostridium sulfidigenes sp. nov., a mesophilic, proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from pond sediment.	Sallam A, Steinbuchel A	Int J Syst Evol Microbiol	10.1099/ijs.0.004986-0	2009
Phylogeny	Clostridium schirmacherense sp. nov., an obligately anaerobic, proteolytic, psychrophilic bacterium isolated from lake sediment of Schirmacher Oasis, Antarctica.	Alam SI, Dixit A, Reddy GSN, Dube S, Palit M, Shivaji S, Singh L	Int J Syst Evol Microbiol	10.1099/ijs.0.63808-0	2006
Phylogeny	Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor.	Hernandez-Eugenio G, Fardeau ML, Cayol JL, Patel BKC, Thomas P, Macarie H, Garcia JL, Ollivier B	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1461	2002

References

#2930	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6970
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#47506	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 21841
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68367	Automatically annotated from API 20A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Clostridium subterminale (DSM 6970, ATCC 25774, CDC KA152)

Name and taxonomic classification

Morphology