Name: Clostridium saccharoperbutylacetonicum N1-4(HMT)
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2659

Type strain:

Species: Clostridium saccharoperbutylacetonicum

Strain Designation: N1-4(HMT)

Culture col. no.: DSM 14923, ATCC 27021, NCIMB 12606

Strain history: <- ATCC <- S. Ogata; N1-4(HMT) <- M. Hongo and K. Nagata, Kyushu University, Fukuoka, Japan {1959}

NCBI tax ID(s): 36745 (species)

SI-ID 44738

ATCC 27021, NCIB 12606, NCIMB 12606, CECT 4187, DSM 14923

Other strains from Clostridium saccharoperbutylacetonicum

C. saccharoperbutylacetonicum N1-504, DSM 2152, ATCC 27022

Section

Clostridium saccharoperbutylacetonicum N1-4 is an anaerobe, mesophilic bacterium that was isolated from soil.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium saccharoperbutylacetonicum**
	Full Scientific Name (LPSN)	Clostridium saccharoperbutylacetonicum Keis et al. 2001

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5607]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #5607]

Culture medium growth

[Ref.: #5607]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5607]

Culture medium composition

expand / minimize

[Ref.: #5607]

Culture medium

PY + X MEDIUM (DSMZ Medium 104b)

[Ref.: #5607]

Culture medium growth

[Ref.: #5607]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5607]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5607]			growth	30 °C

[Ref.: #5607]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5607]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5607]	Sample type/isolated from	soil
[Ref.: #5607]	Geographic location (country and/or sea, region)	Fukuoka
[Ref.: #5607]	Country	Japan
[Ref.: #5607]	Country ISO 3 Code	JPN
[Ref.: #5607]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5607]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5607]	Clostridium saccharoperbutylacetonicum N1-4 16S ribosomal RNA (rrn) gene, partial sequence	U16122	1417	ENA	931276 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium saccharoperbutylacetonicum N1-4(HMT)	GCA_000340885	complete	GenBank	931276 tax ID	*
[Ref.: #66792]	Clostridium saccharoperbutylacetonicum N1-4(HMT) ATCC 27021	GCA_000334435	contig	GenBank	931276 tax ID	*
[Ref.: #66792]	Clostridium saccharoperbutylacetonicum N1-4(HMT) strain ATCC 27021	931276.4	wgs	PATRIC	931276 tax ID	*
[Ref.: #66792]	Clostridium saccharoperbutylacetonicum N1-4(HMT) strain N1-4(HMT)	931276.5	complete	PATRIC	931276 tax ID	*
[Ref.: #66792]	Clostridium saccharoperbutylacetonicum N1-4(HMT) strain N1-4(HMT) strain N1-4(HMT) strain N1-4(HMT)	931276.25	plasmid	PATRIC	931276 tax ID	*
[Ref.: #66792]	Clostridium saccharoperbutylacetonicum N1-4(HMT)	2608642274	complete	JGI IMG/M	931276 tax ID	*

[Ref.: #5607]

GC-content

31 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium saccharoperbutylacetonicum N1-4(HMT) N1-4(HMT) NCBI: GCA_000340885.1
[Ref.: #69480]	motile	yes	80.715	no
[Ref.: #69480]	flagellated	yes	64.484	no
[Ref.: #69480]	gram-positive	yes	61.995	no
[Ref.: #69480]	anaerobic	yes	95.214	no
[Ref.: #69480]	aerobic	no	96.355	no
[Ref.: #69480]	halophile	no	96.988	no
[Ref.: #69480]	spore-forming	yes	93.989	no
[Ref.: #69480]	glucose-util	yes	89.813	no
[Ref.: #69480]	thermophile	no	99.58	no
[Ref.: #69480]	glucose-ferment	yes	70.272	no

Availability in culture collections External links

[Ref.: #5607]

Culture collection no.

DSM 14923, ATCC 27021, NCIMB 12606

[Ref.: #72188]

SI-ID 44738

Literature:

	Title	Authors	Journal	DOI	Year
Metabolism	Genome sequence analysis of the temperate bacteriophage TBP2 of the solvent producer Clostridium saccharoperbutylacetonicum N1-4 (HMT, ATCC 27021).	Schuler MA, Stegmann BA, Poehlein A, Daniel R, Durre P	FEMS Microbiol Lett	10.1093/femsle/fnaa103	2020	*
Metabolism	Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4.	Herman NA, Li J, Bedi R, Turchi B, Liu X, Miller MJ, Zhang W	Appl Environ Microbiol	10.1128/AEM.02942-16	2016	*
Genetics	Complete Genome Sequence of the Solvent Producer Clostridium saccharoperbutylacetonicum Strain DSM 14923.	Poehlein A, Krabben P, Durre P, Daniel R	Genome Announc	10.1128/genomeA.01056-14	2014	*
Phylogeny	Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov.	Keis S, Shaheen R, Jones DT	Int J Syst Evol Microbiol	10.1099/00207713-51-6-2095	2001	*
	Three-stage repeated-batch immobilized cell fermentation to produce butanol from non-detoxified sugarcane bagasse hemicellulose hydrolysates.	Chacon SJ, Matias G, Ezeji TC, Maciel Filho R, Mariano AP	Bioresour Technol	10.1016/j.biortech.2020.124504	2020	*

References

#5607

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14923

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#72188

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44738.1 )

* These data were automatically processed and therefore are not curated

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Clostridium saccharoperbutylacetonicum N1-4(HMT) N1-4(HMT)

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