Name: Clostridium cellulovorans 743B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2568

Type strain:

Species: Clostridium cellulovorans

Strain Designation: 743B

Culture col. no.: DSM 3052, ATCC 35296, OCM 3

Strain history: <- R. A. Mah, University of California, School of Public Health, Los Angeles, USA; 743B

NCBI tax ID(s): 1493 (species)

SI-ID 40980

ATCC 35296, DSM 3052, KCTC 3043, BCRC 14498, CCRC 14498

Section

Clostridium cellulovorans 743B is an anaerobe, spore-forming, mesophilic bacterium that forms rhizoid colonies and was isolated from methanogenic fermentation of hybrid poplar wood.

colony-forming
Gram-negative
other
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium cellulovorans**
	Full Scientific Name (LPSN)	Clostridium cellulovorans Sleat et al. 1985

Information on morphological and physiological properties Morphology

[Ref.: #43428]	Gram stain	negative
[Ref.: #43428]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %99.999

[Ref.: #43428]	Cell length	2.5-3.5 µm
[Ref.: #43428]	Cell length	4-7 µm

[Ref.: #43428]	Cell width	0.7-0.9 µm
[Ref.: #43428]	Cell width	1.5-2 µm

[Ref.: #43428]	Cell shape	other
[Ref.: #43428]	Cell shape	other

[Ref.: #43428]	Motility	no
[Ref.: #43428]	Motility	no

[Ref.: #43428]	Flagellum arrangement	peritrichous
[Ref.: #43428]	Flagellum arrangement	peritrichous

[Ref.: #43428]	Colony size	1-2 mm
[Ref.: #43428]	Colony color	creamy-white and opaque
[Ref.: #43428]	Colony shape	rhizoid
[Ref.: #43428]	Incubation period	2 days
[Ref.: #43428]	Cultivation medium used	Cellobiose agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1287]

Culture medium

RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM (DSMZ Medium 520)

[Ref.: #1287]

Culture medium growth

[Ref.: #1287]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1287]

Culture medium composition

expand / minimize

[Ref.: #1287]

Culture medium

CLOSTRIDIUM CELLULOVORANS MEDIUM (DSMZ Medium 320)

[Ref.: #1287]

Culture medium growth

[Ref.: #1287]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1287]

Culture medium composition

expand / minimize

[Ref.: #43428]

Culture medium

Cellobiose agar

[Ref.: #43428]

Culture medium growth

		Temperature
[Ref.: #1287]	growth	37 °C
[Ref.: #43404]	optimum	37 °C
[Ref.: #43428]	growth	20-40 °C

[Ref.: #1287]	Temperature range	mesophilic
[Ref.: #43404]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43428]			optimum	7
[Ref.: #43428]			growth	6.4-7.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1287]	Oxygen tolerance	anaerobe
[Ref.: #43428]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.617

[Ref.: #43404]	Spore description	located either centrally or subterminally
[Ref.: #43404]	Ability of spore formation	yes
[Ref.: #43428]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %93
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43428]	arabinitol ChEBI	-	fermentation
[Ref.: #43428]	arabinose ChEBI	-	fermentation
[Ref.: #43428]	casein	-	hydrolysis
[Ref.: #43404]	cellobiose ChEBI	+	carbon source
[Ref.: #43428]	cellobiose ChEBI	+	fermentation
[Ref.: #43404]	cellulose ChEBI	+	carbon source
[Ref.: #43428]	cellulose ChEBI	+	fermentation
[Ref.: #43428]	dihydrogen ChEBI	+	fermentation
[Ref.: #43428]	erythritol ChEBI	-	fermentation
[Ref.: #43404]	fructose ChEBI	+	carbon source
[Ref.: #43428]	fructose ChEBI	+	fermentation
[Ref.: #43404]	galactose ChEBI	+	carbon source
[Ref.: #43428]	galactose ChEBI	+	fermentation
[Ref.: #43428]	gelatin ChEBI	-	hydrolysis
[Ref.: #43404]	glucose ChEBI	+	carbon source
[Ref.: #43428]	glucose ChEBI	+	fermentation
[Ref.: #43428]	glycerol ChEBI	-	fermentation
[Ref.: #43428]	lactate ChEBI	-	fermentation
[Ref.: #43428]	lactate ChEBI	+	fermentation
[Ref.: #43428]	lactose ChEBI	+	fermentation
[Ref.: #43428]	maltose ChEBI	+	fermentation
[Ref.: #43404]	mannose ChEBI	+	carbon source
[Ref.: #43428]	mannose ChEBI	+	fermentation
[Ref.: #43428]	melezitose ChEBI	-	fermentation
[Ref.: #43404]	pectin ChEBI	+	carbon source
[Ref.: #43428]	pectin ChEBI	+	fermentation
[Ref.: #43428]	pyruvate ChEBI	-	fermentation
[Ref.: #43428]	rhamnose ChEBI	-	fermentation
[Ref.: #43428]	sorbitol ChEBI	-	fermentation
[Ref.: #43428]	starch ChEBI	-	hydrolysis
[Ref.: #43428]	sucrose ChEBI	+	fermentation
[Ref.: #43428]	sulfate ChEBI	-	reduction
[Ref.: #43428]	trehalose ChEBI	-	fermentation
[Ref.: #43404]	xylan ChEBI	+	carbon source
[Ref.: #43428]	xylan ChEBI	+	fermentation
[Ref.: #43428]	xylose ChEBI	-	fermentation
[Ref.: #43428]	yeast extract	+	required for growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43428]	acetate ChEBI	yes
[Ref.: #43428]	butyrate ChEBI	yes
[Ref.: #43428]	carbon dioxide ChEBI	yes
[Ref.: #43428]	dihydrogen ChEBI	yes
[Ref.: #43428]	DL-lactate ChEBI	yes
[Ref.: #43428]	ethanol ChEBI	yes
[Ref.: #43428]	formate ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43428]		catalase	-	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1287]	Sample type/isolated from	methanogenic fermentation of hybrid poplar wood

[Ref.: #43428]	Sample type/isolated from	a batch methanogenic fermentation of finely divided hybrid poplar wood
[Ref.: #43428]	Geographic location (country and/or sea, region)	Institute of Gas Technology, Chicago
[Ref.: #43428]	Country	USA
[Ref.: #43428]	Country ISO 3 Code	USA
[Ref.: #43428]	Continent	North America
* marker position based on {}

Isolation sources categories

#Condition	#Anoxic (anaerobic)	-
#Host	#Plants	-
#Host Body Product	#Plant	#Timber

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X71849 (>99% sequence identity) for Clostridium cellulovorans subclade from Microbeatlas

Aquatic Samples	1721
Soil Samples	3150
Animal Samples	1021
Plant Samples	543
Total Samples	6435

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1287]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1287]	C.cellulovorans gene for 16S ribosomal RNA	X71849	1506	ENA	573061 tax ID
[Ref.: #20218]	Corynebacterium matruchotii clone OCM003 16S ribosomal RNA gene, partial sequence	GU420836	443	ENA	43768 tax ID	*
[Ref.: #20218]	Clostridium cellulovorans 16S rRNA gene, strain DSM 3052	X73438	1513	ENA	573061 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium cellulovorans 743B	GCA_000145275	complete	GenBank	573061 tax ID	*
[Ref.: #66792]	Clostridium cellulovorans 743B	GCA_000180115	scaffold	GenBank	573061 tax ID	*
[Ref.: #66792]	Clostridium cellulovorans 743B	573061.5	complete	PATRIC	573061 tax ID	*
[Ref.: #66792]	Clostridium cellulovorans 743B (Prj:52819)	573061.4	wgs	PATRIC	573061 tax ID	*
[Ref.: #66792]	Clostridium cellulovorans 743B, ATCC 35296	648276630	draft	JGI IMG/M	573061 tax ID	*
[Ref.: #66792]	Clostridium cellulovorans 743B, ATCC 35296	648028016	complete	JGI IMG/M	573061 tax ID	*

[Ref.: #1287]	GC-content	26.0-27.0 mol%
[Ref.: #43428]	GC-content	26-27 mol%	Buoyant density centrifugation (BD)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium cellulovorans 743B PATRIC: 573061.5
[Ref.: #69481]	spore-forming	yes	93	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium cellulovorans 743B 743B NCBI: GCA_000145275.1
[Ref.: #69480]	motile	yes	75.17	yes
[Ref.: #69480]	flagellated	yes	88.574	yes
[Ref.: #69480]	gram-positive	yes	76.606	no
[Ref.: #69480]	anaerobic	yes	96.287	yes
[Ref.: #69480]	aerobic	no	93.942	yes
[Ref.: #69480]	halophile	no	96.699	no
[Ref.: #69480]	spore-forming	yes	95.715	yes
[Ref.: #69480]	thermophile	no	97.261	yes
[Ref.: #69480]	glucose-util	yes	89.067	yes
[Ref.: #69480]	glucose-ferment	yes	78.932	yes

Availability in culture collections External links

[Ref.: #1287]

Culture collection no.

DSM 3052, ATCC 35296, OCM 3

[Ref.: #72101]

SI-ID 40980

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Clostridium composti sp. nov., a new anaerobic bacteria isolated from compost.	Shin Y, Paek J, Son AY, Kim H, Kook JK, Paek WK, Chang YH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003074	2018	*
Enzymology	Characterization of engF, a gene for a non-cellulosomal Clostridium cellulovorans endoglucanase.	Sheweita SA, Ichi-ishi A, Park JS, Liu C, Malburg LM Jr, Doi RH	Gene	10.1016/s0378-1119(96)00544-6	1996	*
Phylogeny	Isolation and Characterization of an Anaerobic, Cellulolytic Bacterium, Clostridium cellulovorans sp. nov.	Sleat R, Mah RA, Robinson R	Appl Environ Microbiol	10.1128/aem.48.1.88-93.1984	1984	*

References

#1287

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3052

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#43404

Yutaka Tamaru, Hideo Miyake, Kouichi Kuroda, Akihito Nakanishi, Yujiro Kawade, Kousuke Yamamoto, Masaaki Uemura, Yasuhiro Fujita, Roy H. Doi, Mitsuyoshi Ueda: Genome Sequence of the Cellulosome-Producing Mesophilic Organism Clostridium cellulovorans 743B. J. Bacteriol. 192: 901 - 902 2010 ( DOI 10.1128/JB.01450-09 , PubMed 19948806 )

#43428

Robert Sleat, Robert A. Mah, Ralph Robinson: Isolation and Characterization of an Anaerobic, Cellulolytic Bacterium, Clostridium cellulovorans sp. nov.. Appl. Environ. Microbiol. 48: 88 - 93 1984

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72101

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40980.1 )

* These data were automatically processed and therefore are not curated

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Clostridium cellulovorans 743B

Clostridium cellulovorans 743B 743B

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