Clostridium beijerinckii A-21 is an anaerobe, mesophilic prokaryote that produces alcohol.
alcohol production anaerobe mesophilic genome sequence
SI-ID 48191
| @ref 20215 |
Last LPSN update
2025-12-16 09:47:20 |
| Domain Bacillati |
| Phylum Bacillota |
| Class Clostridia |
| Order Eubacteriales |
| Family Clostridiaceae |
| Genus Clostridium |
| Species Clostridium beijerinckii |
| Full scientific name Clostridium beijerinckii Donker 1926 (Approved Lists 1980) |
| Synonyms (1) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 2661 | CLOSTRIDIUM ACETOBUTYLICUM MEDIUM (DSMZ Medium 411) | Medium recipe at MediaDive  | Name: CLOSTRIDIUM ACETOBUTYLICUM MEDIUM (DSMZ Medium 411) Composition: Potato 200.0 g/l D-Glucose 6.0 g/l CaCO3 2.0 g/l L-Cysteine HCl x H2O 0.5 g/l Sodium resazurin 0.0005 g/l Distilled water | ||
| 2661 | PY + X MEDIUM (DSMZ Medium 104b) | Medium recipe at MediaDive | Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water | ||
| 2661 | CLOSTRIDIUM SP. MEDIUM (DSMZ Medium 54b) | Medium recipe at MediaDive | Name: CLOSTRIDIUM SP. MEDIUM (DSMZ Medium 54b) Composition: CaCO3 20.0 g/l Glucose 20.0 g/l Agar 17.0 g/l (optional) Yeast extract 10.0 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 2661 | positive | growth | 35 | mesophilic |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | C. beijerinckii DSM 6423 assembly for Clostridium beijerinckii | complete | GCA_900010805   | 1520.273  | 2870135238  | 1520 | 93.53 |  |
| 66792 | Clostridium beijerinckii A-21 | complete | 2703719306 | 1520 | 38.51 | | ||
| 124043 | ASM200632v1 assembly for Clostridium beijerinckii NRRL B-593 | contig | GCA_002006325 | 1520 | 4.78 | |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Genetics | CRISPR/anti-CRISPR genome editing in Clostridium beijerinckii. | Hocq R, Chartier G, Lopes Ferreira N, Wasels F. | J Biotechnol | 10.1016/j.jbiotec.2025.11.002 | 2026 | |
| Heterologous 1,3-Propanediol Production Using Different Recombinant Clostridium beijerinckii DSM 6423 Strains. | Schoch T, Baur T, Kunz J, Stoferle S, Durre P. | Microorganisms | 10.3390/microorganisms11030784 | 2023 | | |
| Propanol production through microbial fermentation of biomass. | Sun C, Yang L, Zhao C, Li H, Deng Z, Liu G, Wu C. | Bioresour Bioprocess | 10.1186/s40643-025-00966-6 | 2025 | | |
| Heterologous Production of Isopropanol Using Metabolically Engineered Acetobacterium woodii Strains. | Hofele F, Schoch T, Oberlies C, Durre P. | Bioengineering (Basel) | 10.3390/bioengineering10121381 | 2023 | | |
| Biotechnology | An artificial coculture fermentation system for industrial propanol production. | Hocq R, Sauer M. | FEMS Microbes | 10.1093/femsmc/xtac013 | 2022 | |
| Cellulose hydrolysis and IBE fermentation of eucalyptus sawdust for enhanced biobutanol production by Clostridium beijerinckii DSM 6423 | Cebreiros F, Ferrari MD, Lareo C. | Industrial crops and products. | 10.1016/j.indcrop.2019.03.059 | 2019 | | |
| Comparison of different small molecular weight alcohols for sustaining sulfidogenic bioreactors maintained at moderately low pH. | Santos AL, Johnson DB. | Front Bioeng Biotechnol | 10.3389/fbioe.2022.937987 | 2022 | | |
| Erratum to: Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling. | Mate de Gerando H, Fayolle-Guichard F, Rudant L, Millah SK, Monot F, Lopes Ferreira N, Lopez-Contreras AM. | Appl Microbiol Biotechnol | 10.1007/s00253-017-8505-0 | 2017 | | |
| The exceptional form and function of the giant bacterium Ca. Epulopiscium viviparus revolves around its sodium motive force. | Sannino DR, Arroyo FA, Pepe-Ranney C, Chen W, Volland JM, Elisabeth NH, Angert ER. | Proc Natl Acad Sci U S A | 10.1073/pnas.2306160120 | 2023 | | |
| Biobutanol production from coffee silverskin. | Hijosa-Valsero M, Garita-Cambronero J, Paniagua-Garcia AI, Diez-Antolinez R. | Microb Cell Fact | 10.1186/s12934-018-1002-z | 2018 | | |
| An inventory of early branch points in microbial phosphonate biosynthesis. | Li S, Horsman GP. | Microb Genom | 10.1099/mgen.0.000781 | 2022 | | |
| Investigation on L-rhamnose metabolism of Loigolactobacillus coryniformis subsp. coryniformis DSM 20001 and its propionate-containing fermentates. | Elvan Gezer M, Gravlund Fonss K, Bambace MF, Marietou A, Sandberg Overby S, Sundekilde U, Schwab C. | Appl Environ Microbiol | 10.1128/aem.01613-24 | 2025 | | |
| The potential of native and engineered Clostridia for biomass biorefining. | Ponsetto P, Sasal EM, Mazzoli R, Valetti F, Gilardi G. | Front Bioeng Biotechnol | 10.3389/fbioe.2024.1423935 | 2024 | | |
| Genetics | Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives. | Vees CA, Neuendorf CS, Pflugl S. | J Ind Microbiol Biotechnol | 10.1007/s10295-020-02296-2 | 2020 | |
| Enzymology | A CRISPR/Anti-CRISPR Genome Editing Approach Underlines the Synergy of Butanol Dehydrogenases in Clostridium acetobutylicum DSM 792. | Wasels F, Chartier G, Hocq R, Lopes Ferreira N. | Appl Environ Microbiol | 10.1128/aem.00408-20 | 2020 | |
| Genetics | Comparative genome analysis of commensal segmented filamentous bacteria (SFB) from turkey and murine hosts reveals distinct metabolic features. | Hedblom GA, Dev K, Bowden SD, Baumler DJ. | BMC Genomics | 10.1186/s12864-022-08886-x | 2022 | |
| Genome sequence of the H2-producing Clostridium beijerinckii strain Br21 isolated from a sugarcane vinasse treatment plant. | Fonseca BC, Riano-Pachon DM, Guazzaroni ME, Reginatto V. | Genet Mol Biol | 10.1590/1678-4685-gmb-2017-0315 | 2019 | | |
| Phylogeny | Microbial solvent formation revisited by comparative genome analysis. | Poehlein A, Solano JDM, Flitsch SK, Krabben P, Winzer K, Reid SJ, Jones DT, Green E, Minton NP, Daniel R, Durre P. | Biotechnol Biofuels | 10.1186/s13068-017-0742-z | 2017 | |
| Immobilization of Providencia stuartii Cells in Pumice Stone and Its Application for N-Acetylglucosamine Production. | Halim Y, Devianita D, Hardoko H, Handayani R, Soedirga LC. | Food Technol Biotechnol | 10.17113/ftb.60.01.22.6994 | 2022 | | |
| Effective isopropanol-butanol (IB) fermentation with high butanol content using a newly isolated Clostridium sp. A1424. | Youn SH, Lee KM, Kim KY, Lee SM, Woo HM, Um Y. | Biotechnol Biofuels | 10.1186/s13068-016-0650-7 | 2016 | | |
| Metabolism | Metabolic engineering of microorganisms for the production of higher alcohols. | Choi YJ, Lee J, Jang YS, Lee SY. | mBio | 10.1128/mbio.01524-14 | 2014 | |
| Metabolism | Microbial production of short chain diols. | Jiang Y, Liu W, Zou H, Cheng T, Tian N, Xian M. | Microb Cell Fact | 10.1186/s12934-014-0165-5 | 2014 | |
| Metabolism | Cloning, functional expression and characterization of a bifunctional 3-hydroxybutanal dehydrogenase /reductase involved in acetone metabolism by Desulfococcus biacutus. | Frey J, Rusche H, Schink B, Schleheck D. | BMC Microbiol | 10.1186/s12866-016-0899-9 | 2016 | |
| Acetone, Isopropanol, and Butanol Production by Clostridium beijerinckii (syn. Clostridium butylicum) and Clostridium aurantibutyricum. | George HA, Johnson JL, Moore WE, Holdeman LV, Chen JS. | Appl Environ Microbiol | 10.1128/aem.45.3.1160-1163.1983 | 1983 | | |
| Biotechnology | Isopropanol-butanol-ethanol production by cell-immobilized vacuum fermentation. | Ferreira Dos Santos Vieira C, Duzi Sia A, Maugeri Filho F, Maciel Filho R, Pinto Mariano A | Bioresour Technol | 10.1016/j.biortech.2021.126313 | 2021 | |
| Metabolism | Sugarcane bagasse hydrolysates as feedstock to produce the isopropanol-butanol-ethanol fuel mixture: Effect of lactic acid derived from microbial contamination on Clostridium beijerinckii DSM 6423. | Vieira CFDS, Codogno MC, Maugeri Filho F, Maciel Filho R, Mariano AP | Bioresour Technol | 10.1016/j.biortech.2020.124140 | 2020 | |
| Metabolism | Adaptation and application of a two-plasmid inducible CRISPR-Cas9 system in Clostridium beijerinckii. | Diallo M, Hocq R, Collas F, Chartier G, Wasels F, Wijaya HS, Werten MWT, Wolbert EJH, Kengen SWM, van der Oost J, Ferreira NL, Lopez-Contreras AM | Methods | 10.1016/j.ymeth.2019.07.022 | 2019 | |
| Metabolism | sigma(54) (sigma(L)) plays a central role in carbon metabolism in the industrially relevant Clostridium beijerinckii. | Hocq R, Bouilloux-Lafont M, Lopes Ferreira N, Wasels F | Sci Rep | 10.1038/s41598-019-43822-2 | 2019 | |
| Metabolism | l-Rhamnose Metabolism in Clostridium beijerinckii Strain DSM 6423. | Diallo M, Simons AD, van der Wal H, Collas F, Houweling-Tan B, Kengen SWM, Lopez-Contreras AM | Appl Environ Microbiol | 10.1128/AEM.02656-18 | 2019 | |
| Characterization and genome analysis of a butanol-isopropanol-producing Clostridium beijerinckii strain BGS1. | Zhang C, Li T, He J | Biotechnol Biofuels | 10.1186/s13068-018-1274-x | 2018 | | |
| Transcriptome | Correction to: Genome and transcriptome of the natural isopropanol producer Clostridium beijerinckii DSM6423. | de Gerando HM, Wasels F, Bisson A, Clement B, Bidard F, Jourdier E, Lopez-Contreras AM, Ferreira NL | BMC Genomics | 10.1186/s12864-018-4799-2 | 2018 | |
| Transcriptome | Genome and transcriptome of the natural isopropanol producer Clostridium beijerinckii DSM6423. | Mate de Gerando H, Wasels F, Bisson A, Clement B, Bidard F, Jourdier E, Lopez-Contreras AM, Lopes Ferreira N | BMC Genomics | 10.1186/s12864-018-4636-7 | 2018 | |
| Enzymology | Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling. | Gerando HM, Fayolle-Guichard F, Rudant L, Millah SK, Monot F, Lopes Ferreira N, Lopez-Contreras AM | Appl Microbiol Biotechnol | 10.1007/s00253-016-7302-5 | 2016 | |
| Metabolism | Wood pulp as an immobilization matrix for the continuous production of isopropanol and butanol. | Survase SA, van Heiningen A, Granstrom T | J Ind Microbiol Biotechnol | 10.1007/s10295-012-1219-5 | 2012 | |
| Metabolism | Metabolic engineering of Clostridium acetobutylicum ATCC 824 for isopropanol-butanol-ethanol fermentation. | Lee J, Jang YS, Choi SJ, Im JA, Song H, Cho JH, Seung do Y, Papoutsakis ET, Bennett GN, Lee SY | Appl Environ Microbiol | 10.1128/AEM.06382-11 | 2011 | |
| Metabolism | Continuous production of isopropanol and butanol using Clostridium beijerinckii DSM 6423. | Survase SA, Jurgens G, van Heiningen A, Granstrom T | Appl Microbiol Biotechnol | 10.1007/s00253-011-3322-3 | 2011 | |
| Biotechnology | Continuous production of 1,3-propanediol using raw glycerol with immobilized Clostridium beijerinckii NRRL B-593 in comparison to suspended culture. | Gungormusler M, Gonen C, Azbar N | Bioprocess Biosyst Eng | 10.1007/s00449-011-0522-2 | 2011 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive2554.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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