Name: Caloramator mitchellensis VF08
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2524

Type strain:

Species: Caloramator mitchellensis

Strain Designation: VF08

Culture col. no.: DSM 25793, JCM 15828, KCTC 5735

Strain history: <- CD Ogg and BKC Patel, Griffith Univ., Brisbane, Australia, VF08

NCBI tax ID(s): 908809 (species)

SI-ID 407020

DSM 25793

Section

Caloramator mitchellensis VF08 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from geothermal waters.

Gram-negative
motile
rod-shaped
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Caloramator*
	Species	**Caloramator mitchellensis**
	Full Scientific Name (LPSN)	Caloramator mitchellensis Ogg and Patel 2011

Information on morphological and physiological properties Morphology

[Ref.: #29876]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #29876]	Cell length	2 µm

[Ref.: #29876]	Cell width	0.6 µm

[Ref.: #29876]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	curved-shaped

[Ref.: #29876]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #67771]	Flagellum arrangement	peritrichous

Information on culture and growth conditions Culture and growth conditions

[Ref.: #14356]

Culture medium

PY + X MEDIUM (DSMZ Medium 104b)

[Ref.: #14356]

Culture medium growth

[Ref.: #14356]

Culture medium link

Medium recipe at MediaDive

[Ref.: #14356]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #14356]	growth	50 °C
[Ref.: #29876]	growth	37-60 °C
[Ref.: #29876]	optimum	55 °C
[Ref.: #67770]	growth	50 °C
[Ref.: #67771]	growth	55 °C

[Ref.: #14356]	Temperature range	thermophilic
[Ref.: #29876]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #29876]			optimum	7
[Ref.: #29876]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #14356]	Oxygen tolerance	anaerobe
[Ref.: #29876]	Oxygen tolerance	anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #29876]	Ability of spore formation	no
[Ref.: #67771]	Ability of spore formation	no

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29876]		NaCl		growth	0-2	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29876]	cellobiose ChEBI	+	carbon source
[Ref.: #29876]	fructose ChEBI	+	carbon source
[Ref.: #29876]	glucose ChEBI	+	carbon source
[Ref.: #29876]	maltose ChEBI	+	carbon source
[Ref.: #29876]	nitrate ChEBI	+	reduction
[Ref.: #29876]	pyruvate ChEBI	+	carbon source
[Ref.: #29876]	raffinose ChEBI	+	carbon source
[Ref.: #29876]	ribose ChEBI	+	carbon source
[Ref.: #29876]	serine ChEBI	+	carbon source
[Ref.: #29876]	sucrose ChEBI	+	carbon source
[Ref.: #29876]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	aminopropanol phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	starch degradation	90	9 of 10
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	palmitate biosynthesis	86.36	19 of 22
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	purine metabolism	85.11	80 of 94
[Ref.: #66794]	vitamin B1 metabolism	84.62	11 of 13
[Ref.: #66794]	pyrimidine metabolism	84.44	38 of 45
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	vitamin B12 metabolism	76.47	26 of 34
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	lactate fermentation	75	3 of 4
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	alanine metabolism	72.41	21 of 29
[Ref.: #66794]	tetrahydrofolate metabolism	71.43	10 of 14
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	66.67	6 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	isoleucine metabolism	62.5	5 of 8
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	phenylalanine metabolism	61.54	8 of 13
[Ref.: #66794]	non-pathway related	60.53	23 of 38
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	serine metabolism	55.56	5 of 9
[Ref.: #66794]	nitrate assimilation	55.56	5 of 9
[Ref.: #66794]	urea cycle	53.85	7 of 13
[Ref.: #66794]	methionine metabolism	53.85	14 of 26
[Ref.: #66794]	histidine metabolism	51.72	15 of 29
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	citric acid cycle	50	7 of 14
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	oxidative phosphorylation	47.25	43 of 91
[Ref.: #66794]	degradation of pentoses	46.43	13 of 28
[Ref.: #66794]	d-xylose degradation	45.45	5 of 11
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	tryptophan metabolism	44.74	17 of 38
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	cysteine metabolism	44.44	8 of 18
[Ref.: #66794]	valine metabolism	44.44	4 of 9
[Ref.: #66794]	lysine metabolism	42.86	18 of 42
[Ref.: #66794]	lipid metabolism	41.94	13 of 31
[Ref.: #66794]	factor 420 biosynthesis	40	2 of 5
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	sulfate reduction	38.46	5 of 13
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	degradation of sugar alcohols	37.5	6 of 16
[Ref.: #66794]	metabolism of disaccharids	36.36	4 of 11
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	polyamine pathway	34.78	8 of 23
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	degradation of sugar acids	32	8 of 25
[Ref.: #66794]	leucine metabolism	30.77	4 of 13
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	propionate fermentation	30	3 of 10
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	methanogenesis from CO2	25	3 of 12
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
[Ref.: #66794]	ascorbate metabolism	22.73	5 of 22
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
[Ref.: #66794]	tyrosine metabolism	21.43	3 of 14
[Ref.: #66794]	arginine metabolism	20.83	5 of 24
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #14356]	Sample type/isolated from	geothermal waters
[Ref.: #14356]	Geographic location (country and/or sea, region)	Queensland, Mitchell, Great Artesian Basin bore
[Ref.: #14356]	Country	Australia
[Ref.: #14356]	Country ISO 3 Code	AUS
[Ref.: #14356]	Continent	Australia and Oceania

[Ref.: #67771]	Sample type/isolated from	From water sample collected from a Great Artesian Basin bore
[Ref.: #67771]	Country	Australia
[Ref.: #67771]	Country ISO 3 Code	AUS
[Ref.: #67771]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Great Artesian Basin bore
[Ref.: #67770]	Geographic location (country and/or sea, region)	Mitchell, Queensland
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GU723692 (>99% sequence identity) for Caloramator mitchellensis subclade from Microbeatlas

Aquatic Samples	271
Soil Samples	284
Animal Samples	93
Plant Samples	13
Total Samples	661

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #14356]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #14356]	Caloramator mitchellensis 16S ribosomal RNA gene, partial sequence	GU723692	1605	ENA	908809 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67771]	Caloramator mitchellensis VF08	GCA_001440545	contig	GenBank	908809 tax ID
[Ref.: #66792]	Caloramator mitchellensis VF08	2833544911	draft	JGI IMG/M	908809 tax ID	*

[Ref.: #14356]	GC-content	38.4 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #29876]	GC-content	38.4 mol%

Availability in culture collections External links

[Ref.: #14356]

Culture collection no.

DSM 25793, JCM 15828, KCTC 5735

[Ref.: #72058]

SI-ID 407020

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Caloramator mitchellensis sp. nov., a thermoanaerobe isolated from the geothermal waters of the Great Artesian Basin of Australia, and emended description of the genus Caloramator.	Ogg CD, Patel BKC	Int J Syst Evol Microbiol	10.1099/ijs.0.023655-0	2010	*

References

#14356

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25793

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29876

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26250 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72058

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407020.1 )

#26250

IJSEM 644 2011 ( DOI 10.1099/ijs.0.023655-0 , PubMed 20400665 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Caloramator mitchellensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF