Name: Caloramator australicus RC3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2523

Type strain:

Species: Caloramator australicus

Strain Designation: RC3

Culture col. no.: DSM 22476, JCM 15081, KCTC 5601

Strain history: <- Christopher Ogg ,Griffith Univ, Australia

NCBI tax ID(s): 857293 (strain), 515264 (species)

Section

Caloramator australicus RC3 is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from microbial mat sample.

Gram-positive
motile
rod-shaped
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Caloramator*
	Species	**Caloramator australicus**
	Full Scientific Name (PNU)	Caloramator australicus Ogg and Patel 2009

Information on morphological and physiological properties Morphology

[Ref.: #28898]	Gram stain	positive
[Ref.: #28898]	Cell length	2.5-4.2 µm
[Ref.: #28898]	Cell width	0.8-1 µm
[Ref.: #28898]	Cell shape	rod-shaped
[Ref.: #28898]	Motility	yes

[Ref.: #67771]	Gram stain	positive

[Ref.: #67771]	Cell shape	rod-shaped
[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #25338]	Flagellum arrangement	peritrichous

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16258]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #16258]

Culture medium growth

[Ref.: #16258]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #16258]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16258]	growth	60 °C
[Ref.: #28898]	growth	45-70 °C
[Ref.: #28898]	optimum	60 °C
[Ref.: #67770]	growth	60 °C
[Ref.: #67771]	growth	60 °C

[Ref.: #16258]	Temperature range	thermophilic
[Ref.: #28898]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #28898]			optimum	7
[Ref.: #28898]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16258]	Oxygen tolerance	anaerobe
[Ref.: #28898]	Oxygen tolerance	anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #28898]	Ability of spore formation	no
[Ref.: #67771]	Ability of spore formation	no

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #28898]		NaCl		growth	0-2	%

[Ref.: #25338]

Observation

grew poorly in the presence of 500 µg/ml sodium azide 21 (43 % inhibition)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25338]	acetate ChEBI	-	assimilation
[Ref.: #25338]	alanine ChEBI	-	assimilation
[Ref.: #25338]	amylose ChEBI	+	assimilation
[Ref.: #25338]	arabinose ChEBI	-	assimilation
[Ref.: #25338]	arginine ChEBI	-	assimilation
[Ref.: #25338]	aspartate ChEBI	-	assimilation
[Ref.: #25338]	benzoate ChEBI	-	assimilation
[Ref.: #25338]	carbon dioxide ChEBI	-	assimilation
[Ref.: #25338]	carboxymethylcellulose ChEBI	-	assimilation
[Ref.: #25338]	casamino acids	-	assimilation
[Ref.: #25338]	casein	-	assimilation
[Ref.: #28898]	cellobiose ChEBI	+	carbon source
[Ref.: #25338]	cellulose ChEBI	+	assimilation
[Ref.: #25338]	chitin ChEBI	-	assimilation
[Ref.: #25338]	dihydrogen ChEBI	-	assimilation
[Ref.: #25338]	elemental sulfur ChEBI	+	electron acceptor
[Ref.: #25338]	ethanol ChEBI	-	assimilation
[Ref.: #25338]	ferric iron ChEBI	+	electron acceptor
[Ref.: #25338]	formate ChEBI	-	assimilation
[Ref.: #28898]	fructose ChEBI	+	carbon source
[Ref.: #25338]	fructose ChEBI	+	fermentation
[Ref.: #28898]	galactose ChEBI	+	carbon source
[Ref.: #25338]	galactose ChEBI	+	fermentation
[Ref.: #28898]	glucose ChEBI	+	carbon source
[Ref.: #25338]	glucose ChEBI	+	fermentation
[Ref.: #25338]	glutamine ChEBI	-	assimilation
[Ref.: #25338]	glycerol ChEBI	-	assimilation
[Ref.: #25338]	glycine ChEBI	-	assimilation
[Ref.: #25338]	isoleucine ChEBI	-	assimilation
[Ref.: #25338]	lactate ChEBI	-	assimilation
[Ref.: #25338]	lactose ChEBI	-	assimilation
[Ref.: #25338]	leucine ChEBI	-	assimilation
[Ref.: #28898]	maltose ChEBI	+	carbon source
[Ref.: #25338]	maltose ChEBI	+	fermentation
[Ref.: #25338]	manganese ChEBI	+	electron acceptor
[Ref.: #25338]	mannitol ChEBI	-	assimilation
[Ref.: #28898]	mannose ChEBI	+	carbon source
[Ref.: #25338]	methionine ChEBI	-	assimilation
[Ref.: #25338]	myo-inositol ChEBI	-	assimilation
[Ref.: #25338]	nitrate ChEBI	-	electron acceptor
[Ref.: #25338]	nitrite ChEBI	-	electron acceptor
[Ref.: #25338]	peptone	+	assimilation
[Ref.: #25338]	propionate ChEBI	-	assimilation
[Ref.: #28898]	pyruvate ChEBI	+	carbon source
[Ref.: #28898]	raffinose ChEBI	+	carbon source
[Ref.: #25338]	ribose ChEBI	-	assimilation
[Ref.: #25338]	serine ChEBI	-	assimilation
[Ref.: #25338]	sodium azide ChEBI	-	growth
[Ref.: #25338]	starch ChEBI	+	assimilation
[Ref.: #25338]	succinate ChEBI	-	assimilation
[Ref.: #28898]	sucrose ChEBI	+	carbon source
[Ref.: #25338]	sucrose ChEBI	+	fermentation
[Ref.: #25338]	sulfate ChEBI	-	electron acceptor
[Ref.: #25338]	sulfite ChEBI	-	electron acceptor
[Ref.: #25338]	thiosulfate ChEBI	-	electron acceptor
[Ref.: #28898]	threonine ChEBI	+	carbon source
[Ref.: #25338]	tryptone	-	carbon source
[Ref.: #25338]	tryptone	-	required for growth
[Ref.: #25338]	tryptone	+	assimilation
[Ref.: #25338]	xylan ChEBI	+	assimilation
[Ref.: #28898]	xylose ChEBI	+	carbon source
[Ref.: #25338]	yeast extract (0.01 %, w/v)	+	carbon source
[Ref.: #25338]	yeast extract (0.01 %, w/v)	+	required for growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #25338]	ampicillin ChEBI	yes	10	µg/mL
[Ref.: #25338]	chloramphenicol ChEBI	yes	100	µg/mL
[Ref.: #25338]	penicillin ChEBI	yes	100	µg/mL
[Ref.: #25338]	streptomycin ChEBI	yes	100	µg/mL
[Ref.: #25338]	tetracycline ChEBI	yes	10	µg/mL

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	aminopropanol phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	hydrogen production	100	5 of 5
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	d-mannose degradation	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	urea cycle	84.62	11 of 13
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	starch degradation	80	8 of 10
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	vitamin B12 metabolism	79.41	27 of 34
[Ref.: #66794]	purine metabolism	78.72	74 of 94
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	pyrimidine metabolism	75.56	34 of 45
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	tetrahydrofolate metabolism	71.43	10 of 14
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	IAA biosynthesis	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	66.67	6 of 9
[Ref.: #66794]	alanine metabolism	65.52	19 of 29
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	isoleucine metabolism	62.5	5 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	phenylalanine metabolism	61.54	8 of 13
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	61.54	8 of 13
[Ref.: #66794]	3-chlorocatechol degradation	60	3 of 5
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	lipid metabolism	58.06	18 of 31
[Ref.: #66794]	reductive acetyl coenzyme A pathway	57.14	4 of 7
[Ref.: #66794]	d-xylose degradation	54.55	6 of 11
[Ref.: #66794]	non-pathway related	52.63	20 of 38
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	citric acid cycle	50	7 of 14
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	methionine metabolism	50	13 of 26
[Ref.: #66794]	oxidative phosphorylation	49.45	45 of 91
[Ref.: #66794]	tryptophan metabolism	47.37	18 of 38
[Ref.: #66794]	degradation of pentoses	46.43	13 of 28
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	lysine metabolism	45.24	19 of 42
[Ref.: #66794]	histidine metabolism	44.83	13 of 29
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	valine metabolism	44.44	4 of 9
[Ref.: #66794]	ubiquinone biosynthesis	42.86	3 of 7
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	ethylmalonyl-CoA pathway	40	2 of 5
[Ref.: #66794]	flavin biosynthesis	40	6 of 15
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	polyamine pathway	39.13	9 of 23
[Ref.: #66794]	cysteine metabolism	38.89	7 of 18
[Ref.: #66794]	proline metabolism	36.36	4 of 11
[Ref.: #66794]	ascorbate metabolism	36.36	8 of 22
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	degradation of sugar alcohols	31.25	5 of 16
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	degradation of sugar acids	28	7 of 25
[Ref.: #66794]	vitamin B6 metabolism	27.27	3 of 11
[Ref.: #66794]	methanogenesis from CO2	25	3 of 12
[Ref.: #66794]	dTDPLrhamnose biosynthesis	25	2 of 8
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	phenol degradation	25	5 of 20
[Ref.: #66794]	arginine metabolism	25	6 of 24
[Ref.: #66794]	leucine metabolism	23.08	3 of 13
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
[Ref.: #66794]	tyrosine metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16258]	Sample type/isolated from	microbial mat sample
[Ref.: #16258]	Geographic location (country and/or sea, region)	Queensland, some 1000 km north-west of Brisbane, near Blackall, outflow of the Great Artesian Basin bore - "the New Lorne Bore"
[Ref.: #16258]	Country	Australia
[Ref.: #16258]	Country ISO 3 Code	AUS
[Ref.: #16258]	Continent	Australia and Oceania
[Ref.: #16258]	Geographic location	-24.9133°/145.1380°

[Ref.: #67771]	Sample type/isolated from	From microbial mat existing in the runoff channel from groundwaters
[Ref.: #67771]	Geographic location (country and/or sea, region)	Great Artesian Basin bore, Queensland
[Ref.: #67771]	Country	Australia
[Ref.: #67771]	Country ISO 3 Code	AUS
[Ref.: #67771]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Microbial mat sample from the outflow of a Great Artesian Basin bore in Queensland
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Environmental

#Microbial community

#Microbial mat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU409943 (>99% sequence identity) for Caloramator australicus subclade from Microbeatlas

Aquatic Samples	176
Soil Samples	30
Animal Samples	254
Plant Samples	5
Total Samples	465

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16258]	Caloramator australicus strain KCTC 5601 16S ribosomal RNA gene, partial sequence	EU409943	1471	ENA	857293 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Caloramator australicus RC3 RC3	GCA_000297115	contig	GenBank	857293 tax ID
[Ref.: #66792]	Caloramator australicus RC3	857293.11	wgs	PATRIC	857293 tax ID	*
[Ref.: #66792]	Caloramator australicus RC3	2524023184	draft	JGI IMG/M	857293 tax ID	*

[Ref.: #16258]	GC-content	34 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #28898]	GC-content	34 mol%
[Ref.: #67771]	GC-content	33.0-35.0 mol%

Availability in culture collections External links

[Ref.: #16258]

Culture collection no.

DSM 22476, JCM 15081, KCTC 5601

[Ref.: #20218]

Associated Passport(s) in StrainInfo

841771, 836659

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft genome sequence of Caloramator australicus strain RC3T, a thermoanaerobe from the Great Artesian Basin of Australia.	Ogg CD, Patel BK	J Bacteriol	10.1128/JB.00193-11	2011	*
Phylogeny	Caloramator australicus sp. nov., a thermophilic, anaerobic bacterium from the Great Artesian Basin of Australia.	Ogg CD, Patel BK	Int J Syst Evol Microbiol	10.1099/ijs.0.000802-0	2009	*

References

#16258

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22476

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#25338

IJSEM 95 2009 ( DOI 10.1099/ijs.0.000802-0 , PubMed 19126731 )

#28898

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25338 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#25338

IJSEM 95 2009 ( DOI 10.1099/ijs.0.000802-0 , PubMed 19126731 )

* These data were automatically processed and therefore are not curated

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