Tepidimicrobium ferriphilum (DSM 16624, VKM B-2348, SB91)

Name: Tepidimicrobium ferriphilum (DSM 16624, VKM B-2348, SB91)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2514

Type strain

Strain Designation SB91

Culture col. no. DSM 16624 VKM B-2348 SB91

NCBI tax ID(s) 285106

Special Collections DSMZ Literature strains

Links

Tepidimicrobium ferriphilum SB91 is an anaerobe, spore-forming, thermophilic prokaryote that was isolated from sediment of a freshwater hot spring.

spore-forming Gram-positive motile rod-shaped anaerobe thermophilic 16S sequence

Name and taxonomic classification

SI-ID 289985

DSM 16624,VKM B-2348

@ref 20215

Last LPSN update 2025-12-16 09:47:19

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Tissierellales

Family Tissierellaceae

Genus Tepidimicrobium

Species Tepidimicrobium ferriphilum

Full scientific name Tepidimicrobium ferriphilum Slobodkin et al. 2006

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31501	positive	03-07 µm	0.5-0.6 µm	rod-shaped

Pigmentation

31501

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6531	THERMOTERRABACTERIUM MEDIUM (DSMZ Medium 778)		Medium recipe at MediaDive	Name: THERMOTERRABACTERIUM MEDIUM (DSMZ Medium 778; with strain-specific modifications) Composition: Trypticase peptone 9.94036 g/l Na2-9,10-anthraquinone-2,6-disulfonate 8.2008 g/l Na2CO3 2.48509 g/l Yeast extract 0.994036 g/l NH4Cl 0.328032 g/l KCl 0.328032 g/l MgCl2 x 6 H2O 0.328032 g/l KH2PO4 0.328032 g/l CaCl2 x 2 H2O 0.328032 g/l HCl 0.00248509 g/l FeCl2 x 4 H2O 0.00149105 g/l NaOH 0.000497018 g/l NiCl2 x 6 H2O 0.000198807 g/l CoCl2 x 6 H2O 0.000188867 g/l MnCl2 x 4 H2O 9.94036e-05 g/l Pyridoxine hydrochloride 9.94036e-05 g/l ZnCl2 6.95825e-05 g/l Calcium D-(+)-pantothenate 4.97018e-05 g/l (DL)-alpha-Lipoic acid 4.97018e-05 g/l p-Aminobenzoic acid 4.97018e-05 g/l Thiamine HCl 4.97018e-05 g/l Riboflavin 4.97018e-05 g/l Nicotinic acid 4.97018e-05 g/l Na2MoO4 x 2 H2O 3.57853e-05 g/l Folic acid 1.98807e-05 g/l Biotin 1.98807e-05 g/l H3BO3 5.96421e-06 g/l Na2WO4 x 2 H2O 3.97614e-06 g/l Na2SeO3 x 5 H2O 2.98211e-06 g/l CuCl2 x 2 H2O 1.98807e-06 g/l Vitamin B12 9.94036e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6531	positive	growth	50	thermophilic
31501	positive	growth	26-62
31501	positive	optimum	50	thermophilic

Culture pH

@ref	Ability	Type	PH	PH range
31501	positive	optimum	7.5-8
31501	positive	growth	5.5-9.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
6531	anaerobe
31501	anaerobe

Spore formation

31501

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31501	NaCl	positive	growth	0-3.5 %
31501	NaCl	positive	optimum	0-3.5 %

Observation

31501

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31501	16449 ChEBI	alanine	+	carbon source
31501	15428 ChEBI	glycine	+	carbon source
31501	26271 ChEBI	proline	+	carbon source

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Sediment
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6531	sediment of a freshwater hot spring	Buryatiya, Barguzin Valley	Russia	RUS	Asia

Taxonmaps

69479

Global distribution of 16S sequence GQ461826 (>99% sequence identity) for Tepidimicrobium ferriphilum subclade from Microbeatlas

Aquatic Samples	371
Soil Samples	2025
Animal Samples	444
Plant Samples	799
Total Samples	3639

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6531	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Tepidimicrobium ferriphilum strain DSM 16624 16S ribosomal RNA gene, partial sequence	GQ461826	1473		285106
6531	Tepidimicrobium ferriphilum strain SB91 16S ribosomal RNA gene, partial sequence	AY656718	1493		285106

GC content

6531

GC-content (mol%)33

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Denaturing Gradient Gel Electrophoresis Approach for Microbial Shift Analysis in Thermophilic and Mesophilic Anaerobic Digestions.	Pandey P, Chowdhury D, Wang Y.	Gels	10.3390/gels10050339	2024
Metabolism	Characterization of an Anaerobic, Thermophilic, Alkaliphilic, High Lignocellulosic Biomass-Degrading Bacterial Community, ISHI-3, Isolated from Biocompost.	Shikata A, Sermsathanaswadi J, Thianheng P, Baramee S, Tachaapaikoon C, Waeonukul R, Pason P, Ratanakhanokchai K, Kosugi A.	Enzyme Microb Technol	10.1016/j.enzmictec.2018.07.001	2018
Metabolism	Enhancing aerobic composting of food waste by adding hydrolytically active microorganisms.	Mironov V, Zhukov V, Efremova K, Brinton WF.	Front Microbiol	10.3389/fmicb.2024.1487165	2024
	Microbial insights of enhanced anaerobic conversion of syngas into volatile fatty acids by co-fermentation with carbohydrate-rich synthetic wastewater.	Liu C, Wang W, O-Thong S, Yang Z, Zhang S, Liu G, Luo G.	Biotechnol Biofuels	10.1186/s13068-020-01694-z	2020
	Using custom-built primers and nanopore sequencing to evaluate CO-utilizer bacterial and archaeal populations linked to bioH₂ production.	Akacin I, Ersoy S, Doluca O, Gungormusler M.	Sci Rep	10.1038/s41598-023-44357-3	2023
	Unraveling the microbiome of a thermophilic biogas plant by metagenome and metatranscriptome analysis complemented by characterization of bacterial and archaeal isolates.	Maus I, Koeck DE, Cibis KG, Hahnke S, Kim YS, Langer T, Kreubel J, Erhard M, Bremges A, Off S, Stolze Y, Jaenicke S, Goesmann A, Sczyrba A, Scherer P, Konig H, Schwarz WH, Zverlov VV, Liebl W, Puhler A, Schluter A, Klocke M.	Biotechnol Biofuels	10.1186/s13068-016-0581-3	2016
Phylogeny	Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales.	Slobodkin AI, Tourova TP, Kostrikina NA, Lysenko AM, German KE, Bonch-Osmolovskaya EA, Birkeland NK	Int J Syst Evol Microbiol	10.1099/ijs.0.63694-0	2006

References

#6531	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16624
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#31501	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27799 (see below)
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Tepidimicrobium ferriphilum (DSM 16624, VKM B-2348, SB91)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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