Name: Melioribacter roseus P3M-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24801

Type strain:

Species: Melioribacter roseus

Strain Designation: P3M-2

Culture col. no.: DSM 23840, JCM 17771, VKM B-2668

Strain history: O. A. Podosokorskaya P3M-2.

NCBI tax ID(s): 1134405 (species)

Section

Melioribacter roseus P3M-2 is a facultative anaerobe, organotroph, thermophilic bacterium that was isolated from microbial mat from the wooden surface of a chute under the flow of thermal water coming out of a 2775-m-deep oil exploration well.

Gram-negative
motile
rod-shaped
facultative anaerobe
organotroph
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Chlorobiota*
	Class	*Ignavibacteria*
	Order	*Ignavibacteriales*
	Family	*Melioribacteraceae*
	Genus	*Melioribacter*
	Species	**Melioribacter roseus**
	Full Scientific Name (PNU)	Melioribacter roseus Podosokorskaya et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #43396]	Gram stain	negative
[Ref.: #43396]	Cell length	0.65-10 µm
[Ref.: #43396]	Cell width	0.25-0.35 µm
[Ref.: #43396]	Cell shape	rod-shaped
[Ref.: #43396]	Motility	yes
[Ref.: #43396]	Flagellum arrangement	polar

[Ref.: #43396]	Pigment production	yes
[Ref.: #43396]	Pigment name	carotenoid-type

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21273]

Culture medium

IGNAVIBACTERIA MEDIUM (DSMZ Medium 1374)

[Ref.: #21273]

Culture medium growth

[Ref.: #21273]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #21273]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21273]	growth	47 °C
[Ref.: #43396]	optimum	52-55 °C
[Ref.: #43396]	growth	35-60 °C
[Ref.: #67770]	growth	55 °C

[Ref.: #21273]	Temperature range	thermophilic
[Ref.: #43396]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH	pH range
[Ref.: #43396]			optimum	7.5
[Ref.: #43396]			growth	6-8.7	alkaliphile

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43396]

Oxygen tolerance

facultative anaerobe

[Ref.: #43396]

Nutrition type

organotroph

[Ref.: #43396]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43396]		NaCl		optimum	6	g/L
[Ref.: #43396]		NaCl	no	growth	60	g/L

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43396]	acetate ChEBI	+	assimilation
[Ref.: #43396]	agarose ChEBI	+	assimilation
[Ref.: #43396]	arabinose ChEBI	+	assimilation
[Ref.: #43396]	arsenate ChEBI	+	reduction
[Ref.: #43396]	beef extract	+	assimilation
[Ref.: #43396]	butanol ChEBI	-	assimilation
[Ref.: #43396]	carboxymethylcellulose ChEBI	+	assimilation
[Ref.: #43396]	cellobiose ChEBI	+	assimilation
[Ref.: #43396]	cellulose ChEBI	+	assimilation
[Ref.: #43396]	dextran ChEBI	+	assimilation
[Ref.: #43396]	dioxygen ChEBI	+	reduction
[Ref.: #43396]	ethanol ChEBI	-	assimilation
[Ref.: #43396]	ferrihydrite	+	reduction
[Ref.: #43396]	formate ChEBI	+	assimilation
[Ref.: #43396]	fructose ChEBI	+	assimilation
[Ref.: #43396]	galactose ChEBI	-	assimilation
[Ref.: #43396]	gamma-cyclodextrin ChEBI	+	assimilation
[Ref.: #43396]	gelatin ChEBI	+	assimilation
[Ref.: #43396]	glucose ChEBI	+	assimilation
[Ref.: #43396]	glycogen ChEBI	+	assimilation
[Ref.: #43396]	lactate ChEBI	-	assimilation
[Ref.: #43396]	lactose ChEBI	+	assimilation
[Ref.: #43396]	lichenin ChEBI	+	assimilation
[Ref.: #43396]	lignin ChEBI	-	assimilation
[Ref.: #43396]	maltose ChEBI	+	assimilation
[Ref.: #43396]	mannitol ChEBI	-	assimilation
[Ref.: #43396]	mannose ChEBI	+	assimilation
[Ref.: #43396]	nitrite ChEBI	+	reduction
[Ref.: #43396]	pectin ChEBI	-	assimilation
[Ref.: #43396]	peptone	+	assimilation
[Ref.: #43396]	propanol ChEBI	-	assimilation
[Ref.: #43396]	propionate ChEBI	-	assimilation
[Ref.: #43396]	pyruvate ChEBI	-	assimilation
[Ref.: #43396]	rhamnose ChEBI	+	assimilation
[Ref.: #43396]	ribose ChEBI	+	assimilation
[Ref.: #43396]	sorbitol ChEBI	-	assimilation
[Ref.: #43396]	starch ChEBI	+	assimilation
[Ref.: #43396]	sucrose ChEBI	-	assimilation
[Ref.: #43396]	tetradecane ChEBI	-	assimilation
[Ref.: #43396]	trehalose ChEBI	+	assimilation
[Ref.: #43396]	xanthan ChEBI	+	assimilation
[Ref.: #43396]	xylan ChEBI	+	assimilation
[Ref.: #43396]	xylose ChEBI	+	assimilation
[Ref.: #43396]	yeast extract	+	required for growth
[Ref.: #43396]	yeast extract	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #43396]		acetate ChEBI
[Ref.: #43396]		carbon dioxide ChEBI
[Ref.: #43396]		dihydrogen ChEBI
[Ref.: #43396]		DL-lactate ChEBI

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43396]		catalase	+/-	1.11.1.6
[Ref.: #43396]		cytochrome oxidase	+/-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21273]	Sample type/isolated from	microbial mat from the wooden surface of a chute under the flow of thermal water coming out of a 2775-m-deep oil exploration well
[Ref.: #21273]	Geographic location (country and/or sea, region)	Tomsk region, Parabel’ district, west bank of Ob River
[Ref.: #21273]	Country	Russia
[Ref.: #21273]	Country ISO 3 Code	RUS
[Ref.: #21273]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Microbial mat developing on the wooden surface of a chute under the flow of hot water emerging from a 2775-m-deep oil exploration well in Tomsk region
[Ref.: #67770]	Geographic location (country and/or sea, region)	Western Siberia
[Ref.: #67770]	Country	Russia
[Ref.: #67770]	Country ISO 3 Code	RUS
* marker position based on {}

Isolation sources categories

#Engineered	-	-
#Environmental	#Aquatic	-
#Environmental	#Microbial community	#Microbial mat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JQ292917 (>99% sequence identity) for Melioribacter roseus subclade from Microbeatlas

Aquatic Samples	843
Soil Samples	172
Animal Samples	435
Plant Samples	23
Total Samples	1473

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21273]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21273]	Melioribacter roseus strain P3M-2 16S ribosomal RNA gene, partial sequence	JQ292917	1539	ENA	1191523 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Melioribacter roseus P3M-2 P3M-2	GCA_000279145	complete	GenBank	1191523 tax ID	*
[Ref.: #66792]	Melioribacter roseus P3M-2	1191523.3	complete	PATRIC	1191523 tax ID	*
[Ref.: #67770]	Melioribacter roseus P3M, complete genome	CP003557		ENA	1191523 tax ID
[Ref.: #66792]	Burkholderia pseudomallei HBPUB10269A	GCA_900592695	contig	PATRIC	28450 tax ID	*

[Ref.: #21273]	GC-content	41.3 mol%	sequence analysis
[Ref.: #43396]	GC-content	41 mol%
[Ref.: #67770]	GC-content	41 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #21273]

Culture collection no.

DSM 23840, JCM 17771, VKM B-2668

[Ref.: #20218]

Associated Passport(s) in StrainInfo

891112, 891113

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae.	Podosokorskaya OA, Kadnikov VV, Gavrilov SN, Mardanov AV, Merkel AY, Karnachuk OV, Ravin NV, Bonch-Osmolovskaya EA, Kublanov IV	Environ Microbiol	10.1111/1462-2920.12067	2013	*
Metabolism	Genomic analysis of Melioribacter roseus, facultatively anaerobic organotrophic bacterium representing a novel deep lineage within Bacteriodetes/Chlorobi group.	Kadnikov VV, Mardanov AV, Podosokorskaya OA, Gavrilov SN, Kublanov IV, Beletsky AV, Bonch-Osmolovskaya EA, Ravin NV	PLoS One	10.1371/journal.pone.0053047	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#21273

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23840

#43396

Olga A. Podosokorskaya, Vitaly V. Kadnikov, Sergey N. Gavrilov, Andrey V. Mardanov, Alexander Y. Merkel, Olga V. Karnachuk, Nikolay V. Ravin, Elizaveta A. Bonch-Osmolovskaya, Ilya V. Kublanov: Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae. Environ. Microbiol. 15: 1759 - 1771 2013 ( DOI 10.1111/1462-2920.12067 , PubMed 23297868 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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