Name: Orbus sasakiae C7
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24796

Type strain:

Species: Orbus sasakiae

Strain Designation: C7

Culture col. no.: DSM 27928, JCM 18050, KACC 16544

Strain history: J.-W. Bae C7.

NCBI tax ID(s): 1078475 (species)

SI-ID 408369

DSM 27928

Section

Orbus sasakiae C7 is a facultative anaerobe, Gram-negative, coccus-shaped bacterium that was isolated from gut of butterfly Sasakia charonda.

coccus-shaped
Gram-negative
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Orbales*
	Family	*Orbaceae*
	Genus	*Orbus*
	Species	**Orbus sasakiae**
	Full Scientific Name (LPSN)	Orbus sasakiae Kim et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30679]	Gram stain	negative

[Ref.: #30679]	Cell length	0.75 µm

[Ref.: #30679]	Cell width	0.75 µm

[Ref.: #30679]	Cell shape	coccus-shaped

[Ref.: #30679]	Motility	no

[Ref.: #30679]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21216]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #21216]

Culture medium growth

[Ref.: #21216]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21216]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21216]	growth	20-25 °C
[Ref.: #30679]	growth	04-37 °C
[Ref.: #30679]	optimum	22.5 °C
[Ref.: #67770]	growth	25 °C

[Ref.: #30679]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30679]			optimum	7.5
[Ref.: #30679]			growth	06-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30679]

Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30679]		NaCl		growth	0-5	%
[Ref.: #30679]		NaCl		optimum	1	%

[Ref.: #30679]	Observation	aggregates in clumps
[Ref.: #67770]	Observation	quinones: Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #30679]	cellobiose ChEBI	+	carbon source
[Ref.: #30679]	citrate ChEBI	+	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #30679]	dextrin ChEBI	+	carbon source
[Ref.: #30679]	formate ChEBI	+	carbon source
[Ref.: #30679]	fructose ChEBI	+	carbon source
[Ref.: #30679]	fucose ChEBI	+	carbon source
[Ref.: #30679]	galactose ChEBI	+	carbon source
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #30679]	glucose ChEBI	+	carbon source
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #30679]	lactose ChEBI	+	carbon source
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #30679]	mannose ChEBI	+	carbon source
[Ref.: #30679]	melibiose ChEBI	+	carbon source
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #30679]	methyl D-glucoside ChEBI	+	carbon source
[Ref.: #30679]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #30679]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #30679]	nitrate ChEBI	+	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #30679]	raffinose ChEBI	+	carbon source
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #30679]	succinate ChEBI	+	carbon source
[Ref.: #30679]	sucrose ChEBI	+	carbon source
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #30679]	threonine ChEBI	+	carbon source
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #30679]	tween 40 ChEBI	+	carbon source
[Ref.: #30679]	tween 80 ChEBI	+	carbon source
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #30679]	uridine ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #30679]	acid phosphatase	+	3.1.3.2
[Ref.: #30679]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #30679]	catalase	+	1.11.1.6
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #21216]	16689	+	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	+	+	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21216]	Sample type/isolated from	gut of butterfly Sasakia charonda
[Ref.: #21216]	Host species	Sasakia charonda
[Ref.: #21216]	Country	Republic of Korea
[Ref.: #21216]	Country ISO 3 Code	KOR
[Ref.: #21216]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Gut of the butterfly Sasakia charonda
[Ref.: #67770]	Host species	Sasakia charonda
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JN561614 (>99% sequence identity) for Orbus from Microbeatlas

Aquatic Samples	8
Soil Samples	5
Animal Samples	284
Plant Samples	5
Total Samples	302

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21216]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21216]	Orbus sasakiae strain C7 16S ribosomal RNA gene, partial sequence	JN561614	1301	ENA	1078475 tax ID

[Ref.: #30679]	GC-content	32.1 mol%
[Ref.: #67770]	GC-content	32.1 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #21216]

Culture collection no.

DSM 27928, JCM 18050, KACC 16544

[Ref.: #88618]

SI-ID 408369

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Orbus sasakiae sp. nov., a bacterium isolated from the gut of the butterfly Sasakia charonda, and emended description of the genus Orbus.	Kim JY, Lee J, Shin NR, Yun JH, Whon TW, Kim MS, Jung MJ, Roh SW, Hyun DW, Bae JW	Int J Syst Evol Microbiol	10.1099/ijs.0.041871-0	2012	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21216

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27928

#30679

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27010 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#88618

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408369.1 )

#27010

IJSEM 1766 2013 ( DOI 10.1099/ijs.0.041871-0 , PubMed 22941300 )

* These data were automatically processed and therefore are not curated

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