Name: Exiguobacterium alkaliphilum 12/1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24783

Type strain:

Species: Exiguobacterium alkaliphilum

Strain Designation: 12/1

Culture col. no.: DSM 21148, CCM 8459

Strain history: <- R. Kumar, Institute of Genomics and Integrative Biology, New Delhi, India; 12/1

NCBI tax ID(s): 1428684 (species)

SI-ID 402287

DSM 21148

Section

Exiguobacterium alkaliphilum 12/1 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from alkaline wastewater drainage sludge, beverage industry.

Gram-positive
motile
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Exiguobacterium*
	Species	**Exiguobacterium alkaliphilum**
	Full Scientific Name (LPSN)	Exiguobacterium alkaliphilum Kulshreshtha et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30579]	Gram stain	positive

[Ref.: #30579]	Cell length	2.5 µm

[Ref.: #30579]	Cell width	0.8 µm

[Ref.: #30579]	Cell shape	rod-shaped

[Ref.: #30579]	Motility	yes

[Ref.: #30579]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20560]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #20560]

Culture medium growth

[Ref.: #20560]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20560]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #20560]	growth	37 °C
[Ref.: #30579]	growth	05-40 °C
[Ref.: #30579]	optimum	35 °C

[Ref.: #20560]	Temperature range	mesophilic
[Ref.: #30579]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30579]			optimum	9.5
[Ref.: #30579]			growth	6.5-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30579]

Oxygen tolerance

facultative anaerobe

[Ref.: #30579]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30579]		NaCl		growth	0-9.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30579]	glycogen ChEBI	+	carbon source
[Ref.: #30579]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #30579]	sorbitol ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #30579]		catalase	+	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20560]	Sample type/isolated from	alkaline wastewater drainage sludge, beverage industry
[Ref.: #20560]	Geographic location (country and/or sea, region)	near New Delhi
[Ref.: #20560]	Country	India
[Ref.: #20560]	Country ISO 3 Code	IND
[Ref.: #20560]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Beverage
#Engineered	#Waste	#Industrial wastewater
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Alkaline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU379016 (>99% sequence identity) for Exiguobacterium from Microbeatlas

Aquatic Samples	2262
Soil Samples	574
Animal Samples	3194
Plant Samples	531
Total Samples	6561

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20560]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20560]	Exiguobacterium alkaliphilum 12/1 16S ribosomal RNA gene, partial sequence	EU379016	1477	ENA	506480 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Exiguobacterium alkaliphilum 12/1	GCA_000714435	contig	GenBank	506480 tax ID	*
[Ref.: #66792]	Exiguobacterium alkaliphilum 12/1	506480.4	wgs	PATRIC	506480 tax ID	*
[Ref.: #66792]	Exiguobacterium alkaliphilum 12/1	2585427936	draft	JGI IMG/M	506480 tax ID	*
[Ref.: #66792]	Exiguobacterium alkaliphilum 12_1	GCA_025234775	scaffold	GenBank	1428684 tax ID	*

[Ref.: #20560]	GC-content	56.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30579]	GC-content	56.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Exiguobacterium alkaliphilum 12/1 PATRIC: 506480.4
[Ref.: #69480]	gram-positive	yes	87.491	yes
[Ref.: #69480]	anaerobic	no	98.926	no
[Ref.: #69480]	halophile	yes	81.115	no
[Ref.: #69480]	spore-forming	no	65.257	no
[Ref.: #69480]	glucose-util	yes	89.165	no
[Ref.: #69480]	aerobic	yes	64.513	yes
[Ref.: #69480]	thermophile	no	99.541	yes
[Ref.: #69480]	motile	yes	90.907	yes
[Ref.: #69480]	flagellated	yes	73.334	no
[Ref.: #69480]	glucose-ferment	no	82.492	no

Availability in culture collections External links

[Ref.: #20560]

Culture collection no.

DSM 21148, CCM 8459

[Ref.: #88607]

SI-ID 402287

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Exiguobacterium alkaliphilum sp. nov. isolated from alkaline wastewater drained sludge of a beverage factory.	Mohan Kulshreshtha N, Kumar R, Begum Z, Shivaji S, Kumar A	Int J Syst Evol Microbiol	10.1099/ijs.0.039123-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20560

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21148

#30579

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26910 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#88607

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402287.1 )

#26910

IJSEM 4374 2013 ( DOI 10.1099/ijs.0.039123-0 , PubMed 23838447 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Exiguobacterium alkaliphilum in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF