Name: Neiella marina J221
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24762

Type strain:

Species: Neiella marina

Strain Designation: J221

Culture col. no.: DSM 28678, CGMCC 1.10130, NRRL B-51319

Strain history: <- A. P. Rooney, National Center for Agricultural Utilization Research, Peoria, USA <- Z.-J. Du, Shandong University at Weihai

NCBI tax ID(s): 508461 (species)

SI-ID 405220

DSM 28678

Section

Neiella marina J221 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from intestine of sea cucumber Apostichopus japonicus.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Alteromonadales*
	Family	"Echinimonadaceae"
	Genus	*Neiella*
	Species	**Neiella marina**
	Full Scientific Name (LPSN)	Neiella marina Du et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30738]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.858

[Ref.: #30738]	Cell length	1.6 µm

[Ref.: #30738]	Cell width	0.5 µm

[Ref.: #30738]	Cell shape	rod-shaped

[Ref.: #30738]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.523

[Ref.: #30738]

Pigment production

yes

Multimedia content

[Ref.: #21253]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #21253]	growth	28 °C
[Ref.: #30738]	growth	10-40 °C
[Ref.: #30738]	optimum	26.5 °C

[Ref.: #21253]	Temperature range	mesophilic
[Ref.: #30738]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30738]			optimum	7.75
[Ref.: #30738]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30738]

Oxygen tolerance

aerobe

[Ref.: #30738]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.798

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30738]		NaCl		growth	01-05	%
[Ref.: #30738]		NaCl		optimum	2.5	%

[Ref.: #30738]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30738]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30738]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30738]	mannitol ChEBI	+	carbon source
[Ref.: #30738]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #30738]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30738]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #30738]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21253]	23451	+	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21253]	Sample type/isolated from	intestine of sea cucumber Apostichopus japonicus
[Ref.: #21253]	Host species	Apostichopus japonicus
[Ref.: #21253]	Geographic location (country and/or sea, region)	East China Sea
[Ref.: #21253]	Country	China
[Ref.: #21253]	Country ISO 3 Code	CHN
[Ref.: #21253]	Continent	Asia
[Ref.: #21253]	Geographic location	36.0000°/120.2670°
* marker position based on {}

Isolation sources categories

#Host	#Invertebrates (Other)	#Echinodermata
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21253]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21253]	Neiella marina strain j221 16S ribosomal RNA gene, partial sequence	EU513001	1501	ENA	508461 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Neiella marina J221	GCA_002165625	contig	GenBank	508461 tax ID	*
[Ref.: #66792]	Neiella marina CGMCC 1.10130	GCA_014636195	contig	GenBank	508461 tax ID	*
[Ref.: #66792]	Neiella marina strain CGMCC 1.10130	508461.6	wgs	PATRIC	508461 tax ID	*
[Ref.: #66792]	Neiella marina J221	2886580473	draft	JGI IMG/M	508461 tax ID	*

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GC-content

46.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Neiella marina strain CGMCC 1.10130 PATRIC: 508461.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Neiella marina strain CGMCC 1.10130 PATRIC: 508461.6
[Ref.: #69480]	gram-positive	no	98.801	no
[Ref.: #69480]	anaerobic	no	97.369	yes
[Ref.: #69480]	halophile	yes	52.028	no
[Ref.: #69480]	spore-forming	no	96.746	yes
[Ref.: #69480]	glucose-util	yes	80.51	yes
[Ref.: #69480]	aerobic	yes	75.061	no
[Ref.: #69480]	motile	yes	89.274	no
[Ref.: #69480]	flagellated	yes	85.692	no
[Ref.: #69480]	thermophile	no	99.236	yes
[Ref.: #69480]	glucose-ferment	yes	68.235	yes

Availability in culture collections External links

[Ref.: #21253]

Culture collection no.

DSM 28678, CGMCC 1.10130, NRRL B-51319

[Ref.: #88589]

SI-ID 405220

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Neiella marina gen. nov., sp. nov., isolated from the sea cucumber Apostichopus japonicus.	Du ZJ, Miao TT, Rooney AP, Liu QQ, Chen GJ	Int J Syst Evol Microbiol	10.1099/ijs.0.043448-0	2012	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21253

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28678

#30738

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27069 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88589

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405220.1 )

#27069

IJSEM 1597 2013 ( DOI 10.1099/ijs.0.043448-0 , PubMed 22904222 )

* These data were automatically processed and therefore are not curated

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Neiella marina strain CGMCC 1.10130

Neiella marina strain CGMCC 1.10130

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