Name: Vibrio splendidus AlyHP32
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24663

Type strain:

Species: Vibrio splendidus

Strain Designation: AlyHP32

Culture col. no.: DSM 26178, CECT 8714, KCTC 32085

Strain history: <- D. Kim, Department of Food Science and Technology, Chonnam University, Gwangju <- KCTC <- D. Kim, Department of Food Science and Technology, Chonnam University, Gwangju

NCBI tax ID(s): 29497 (species)

Other strains from Vibrio splendidus

V. splendidus DSM 19640, ATCC 33125, CECT 528, ... (type strain)
V. splendidus CIP 107715
V. splendidus CIP 107716
V. splendidus CCUG 20272, ATCC 33869
V. splendidus CCUG 20273, ATCC 33870
V. splendidus JCM 21194, ATCC 25914, CCUG 20279, ...
V. splendidus 10N.222.46.C2, DSM 113771
V. splendidus 10N.222.46.B6, DSM 113772
V. splendidus 10N.286.45.B3, DSM 113775
V. splendidus 10N.261.48.B5, DSM 113776
V. splendidus 10N.286.45.A10, DSM 113777
V. splendidus 10N.261.52.F2, DSM 113778

Section

Vibrio splendidus AlyHP32 is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from gut of a sea-urchin .

Gram-negative
motile
rod-shaped
animal pathogen
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	"Vibrionales"
	Family	*Vibrionaceae*
	Genus	*Vibrio*
	Species	**Vibrio splendidus**
	Full Scientific Name (PNU)	Vibrio splendidus (Beijerinck 1900) Baumann et al. 1981

	Synonym	Beneckea splendida
	Synonym	"Photobacter* splendidum"*
	Synonym	Vibrio hemicentroti

Information on morphological and physiological properties Morphology

[Ref.: #30937]	Gram stain	negative
[Ref.: #30937]	Cell length	1.75 µm
[Ref.: #30937]	Cell width	0.7 µm
[Ref.: #30937]	Cell shape	rod-shaped
[Ref.: #30937]	Motility	yes

[Ref.: #30937]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21069]

Culture medium

REACTIVATION WITH LIQUID MEDIUM 514 (DSMZ Medium 514c)

[Ref.: #21069]

Culture medium growth

[Ref.: #21069]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #21069]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21069]	growth	25 °C
[Ref.: #30937]	growth	04-30 °C
[Ref.: #30937]	optimum	20 °C

[Ref.: #21069]	Temperature range	mesophilic
[Ref.: #30937]	Temperature range	psychrophilic

	pH	Kind of pH		pH	pH range
[Ref.: #30937]			optimum	8
[Ref.: #30937]			growth	06-10	alkaliphile

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30937]

Oxygen tolerance

facultative anaerobe

[Ref.: #21069]

Name of produced compound

alginolytic enzymes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30937]		NaCl		growth	01-06	%
[Ref.: #30937]		NaCl		optimum	3	%

[Ref.: #30937]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30937]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30937]	dextrin ChEBI	+	carbon source
[Ref.: #30937]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #30937]	fructose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30937]	glucose ChEBI	+	carbon source
[Ref.: #30937]	inosine ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30937]	mannitol ChEBI	+	carbon source
[Ref.: #30937]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #30937]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #30937]	raffinose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	+	energy source	* from API 20NE
[Ref.: #30937]	tween 40 ChEBI	+	carbon source
[Ref.: #30937]	tween 80 ChEBI	+	carbon source
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #30937]		indole ChEBI
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #30937]		indole ChEBI	+
[Ref.: #68369]		indole ChEBI	+	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #30937]	acid phosphatase	+	3.1.3.2
[Ref.: #30937]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #30937]	catalase	+	1.11.1.6
[Ref.: #30937]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #30937]	gelatinase	+
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21069]	20901	+	+	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21069]	Sample type/isolated from	gut of a sea-urchin (Hemicentrotus pulcherimus)
[Ref.: #21069]	Host species	Hemicentrotus pulcherrimus
[Ref.: #21069]	Geographic location (country and/or sea, region)	South Sea (34° 34' N 127° 47' E)
[Ref.: #21069]	Country	Republic of Korea
[Ref.: #21069]	Country ISO 3 Code	KOR
[Ref.: #21069]	Continent	Asia
[Ref.: #21069]	Geographic location	34.5667°/127.7830°
* marker position based on {}

Isolation sources categories

#Host	#Invertebrates (Other)	#Echinodermata
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX204734 (>99% sequence identity) for Vibrio from Microbeatlas

Aquatic Samples	5115
Soil Samples	160
Animal Samples	960
Plant Samples	30
Total Samples	6265

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21069]	Pathogenicity (animal)	yes
[Ref.: #21069]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21069]	Vibrio hemicentroti strain AlyHP32 16S ribosomal RNA gene, partial sequence	JX204734	1455	ENA	29497 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Vibrio splendidus CECT 8714	GCA_900089845	contig	GenBank	29497 tax ID	*
[Ref.: #66792]	Vibrio hemicentroti strain CECT 8714	1220705.6	wgs	PATRIC	29497 tax ID	*
[Ref.: #66792]	Vibrio splendidus CECT 8714	2767802216	draft	JGI IMG/M	29497 tax ID	*

[Ref.: #21069]	GC-content	44.1 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30937]	GC-content	44.1 mol%

Availability in culture collections External links

[Ref.: #21069]

Culture collection no.

DSM 26178, CECT 8714, KCTC 32085

[Ref.: #20218]

Associated Passport(s) in StrainInfo

891270, 891268, 891269

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Vibrio hemicentroti sp. nov., an alginate lyase-producing bacterium, isolated from the gut microflora of sea urchin (Hemicentrotus pulcherrimus).	Kim D, Baik KS, Hwang YS, Choi JS, Kwon J, Seong CN	Int J Syst Evol Microbiol	10.1099/ijs.0.047951-0	2013	*
Phylogeny	Whole genome sequences reveal Vibrio hemicentroti Kim et al. 2013 as a later heterotypic synonym of Vibrio splendidus (Beijerinck 1900) Baumann et al. 1981.	Lucena T, Carmen Macian M, Arahal DR, Rodrigo-Torres L, Pujalte MJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001842	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#21069

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26178

#30937

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27267 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#27267

IJSEM 3697 2013 ( DOI 10.1099/ijs.0.047951-0 , PubMed 23625262 )

* These data were automatically processed and therefore are not curated

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