Name: Thermococcus cleftensis CL1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 24652

Type strain:

Species: Thermococcus cleftensis

Strain Designation: CL1

Culture col. no.: DSM 27260, KACC 17922

Strain history: <- J. Holden, UMASS; CL1

NCBI tax ID(s): 163003 (species)

SI-ID 402342

DSM 27260

Special Collections

DSMZ

Section

Thermococcus cleftensis CL1 is an anaerobe, Gram-negative archaeon that was isolated from polychaete worm of the genus Paralvinella in a fragment of a black smoker.

Gram-negative
anaerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Thermococci*
	Order	*Thermococcales*
	Family	*Thermococcaceae*
	Genus	*Thermococcus*
	Species	**Thermococcus cleftensis**
	Full Scientific Name (LPSN)	Thermococcus cleftensis Hensley et al. 2014

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21061]

Culture medium

MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)

[Ref.: #21061]

Culture medium growth

[Ref.: #21061]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21061]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #21061]			growth	80 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Ability of spore formation

no Confidence in %91.302

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21061]	Sample type/isolated from	polychaete worm of the genus Paralvinella in a fragment of a black smoker
[Ref.: #21061]	Host species	Paralvinella
[Ref.: #21061]	Geographic location (country and/or sea, region)	north-eastern Pacific Ocean, Cleft Segment of the Juan de Fuca Ridge at a depth of 2350 m (44° 30' 00'' N 130° 30' 00'' W)
[Ref.: #21061]	Geographic location	44.5000°/-130.5000°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Host	#Invertebrates (Other)	#Annelida
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21061]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21061]	Thermococcus cleftensis strain CL1 16S ribosomal RNA gene, partial sequence	AF350369	1367	GenBank ENA	163003 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermococcus cleftensis CL1	GCA_000265525	complete	GenBank ENA	163003 tax ID	*
[Ref.: #66792]	Thermococcus cleftensis CL1	2518645553	complete	JGI IMG/M	163003 tax ID	*

[Ref.: #21061]

GC-content

55.8 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermococcus cleftensis CL1 NCBI: GCA_000265525.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	80	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermococcus cleftensis CL1 NCBI: GCA_000265525.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	70.002	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	86.973	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	88.094	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	91.302	no
[Ref.: #69480]	thermophilic	thermophileⓘ	yes	80.71	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	82.458	no

Availability in culture collections External links

[Ref.: #21061]

Culture collection no.

DSM 27260, KACC 17922

[Ref.: #88492]

SI-ID 402342

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermococcus paralvinellae sp. nov. and Thermococcus cleftensis sp. nov. of hyperthermophilic heterotrophs from deep-sea hydrothermal vents.	Hensley SA, Jung JH, Park CS, Holden JF	Int J Syst Evol Microbiol	10.1099/ijs.0.066100-0	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21061

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27260

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88492

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402342.1 )

* These data were automatically processed and therefore are not curated

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