Lihuaxuella thermophila (DSM 46701, CCTCC AA 2011024, JCM 18059)

Name: Lihuaxuella thermophila (DSM 46701, CCTCC AA 2011024, JCM 18059)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24646

Type strain

Culture col. no. DSM 46701 CCTCC AA 2011024 JCM 18059 YIM 77831

NCBI tax ID(s) 1173111

Special Collections DSMZ JCM

History <- JCM; JCM 18059 <- S.-K. Tang; YIM 77831 S.-K. Tang YIM 77831.

Links

Lihuaxuella thermophila DSM 46701 is a thermophilic prokaryote that was isolated from geothermal soil.

thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 397960

JCM 18059,DSM 46701

@ref 20215

Last LPSN update 2026-02-09 11:24:02

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Thermoactinomycetaceae

Genus Lihuaxuella

Species Lihuaxuella thermophila

Full scientific name Lihuaxuella thermophila Yu et al. 2013

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21243	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
21243	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21243	positive	growth	50	thermophilic
67770	positive	growth	50	thermophilic

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125438		94.73
125439		90.5

Observation

67770

Observationquinones: MK-7

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cellulose degradation	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	threonine metabolism	100	10 of 10
66794	gluconeogenesis	100	8 of 8
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	palmitate biosynthesis	100	22 of 22
66794	methylglyoxal degradation	100	5 of 5
66794	lipoate biosynthesis	100	5 of 5
66794	biotin biosynthesis	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	tetrahydrofolate metabolism	100	14 of 14
66794	suberin monomers biosynthesis	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	glycine betaine biosynthesis	100	5 of 5
66794	ribulose monophosphate pathway	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	glutamate and glutamine metabolism	92.86	26 of 28
66794	phenylalanine metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	starch degradation	90	9 of 10
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	d-mannose degradation	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	methionine metabolism	88.46	23 of 26
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	photosynthesis	85.71	12 of 14
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	vitamin B12 metabolism	85.29	29 of 34
66794	vitamin B1 metabolism	84.62	11 of 13
66794	leucine metabolism	84.62	11 of 13
66794	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
66794	Entner Doudoroff pathway	80	8 of 10
66794	glycogen metabolism	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	citric acid cycle	78.57	11 of 14
66794	glutathione metabolism	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	NAD metabolism	77.78	14 of 18
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	acetate fermentation	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	purine metabolism	73.4	69 of 94
66794	proline metabolism	72.73	8 of 11
66794	alanine metabolism	72.41	21 of 29
66794	propanol degradation	71.43	5 of 7
66794	non-pathway related	71.05	27 of 38
66794	coenzyme M biosynthesis	70	7 of 10
66794	degradation of sugar alcohols	68.75	11 of 16
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	flavin biosynthesis	66.67	10 of 15
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	pyrimidine metabolism	66.67	30 of 45
66794	tryptophan metabolism	65.79	25 of 38
66794	glycolysis	64.71	11 of 17
66794	lipid metabolism	64.52	20 of 31
66794	heme metabolism	64.29	9 of 14
66794	tyrosine metabolism	64.29	9 of 14
66794	degradation of sugar acids	64	16 of 25
66794	vitamin B6 metabolism	63.64	7 of 11
66794	d-xylose degradation	63.64	7 of 11
66794	oxidative phosphorylation	62.64	57 of 91
66794	ketogluconate metabolism	62.5	5 of 8
66794	arginine metabolism	62.5	15 of 24
66794	histidine metabolism	62.07	18 of 29
66794	lysine metabolism	61.9	26 of 42
66794	urea cycle	61.54	8 of 13
66794	cysteine metabolism	61.11	11 of 18
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	pantothenate biosynthesis	50	3 of 6
66794	degradation of hexoses	50	9 of 18
66794	glycine metabolism	50	5 of 10
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	selenocysteine biosynthesis	50	3 of 6
66794	toluene degradation	50	2 of 4
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	kanosamine biosynthesis II	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	degradation of pentoses	50	14 of 28
66794	quinate degradation	50	1 of 2
66794	polyamine pathway	47.83	11 of 23
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	metabolism of disaccharids	45.45	5 of 11
66794	lipid A biosynthesis	44.44	4 of 9
66794	nitrate assimilation	44.44	4 of 9
66794	phenol degradation	40	8 of 20
66794	3-chlorocatechol degradation	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	arachidonic acid metabolism	33.33	6 of 18
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	cyanate degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	3-phenylpropionate degradation	33.33	5 of 15
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	enterobactin biosynthesis	33.33	1 of 3
66794	sulfate reduction	30.77	4 of 13
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	ascorbate metabolism	27.27	6 of 22
66794	cholesterol biosynthesis	27.27	3 of 11
66794	catecholamine biosynthesis	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	carotenoid biosynthesis	22.73	5 of 22

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Environmental	#Terrestrial	#Geologic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
21243	geothermal soil	Yunnan province, Tengchong, Rehai National Park	China	CHN	Asia
67770	Geothermal soil from the south-west China

Taxonmaps

69479

Global distribution of 16S sequence JQ750619 (>99% sequence identity) for Lihuaxuella thermophila subclade from Microbeatlas

Aquatic Samples	60
Soil Samples	259
Animal Samples	251
Plant Samples	33
Total Samples	603

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21243	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2675903160 annotated assembly for Lihuaxuella thermophila DSM 46701	scaffold	GCA_900110165	1173111.3	2675903160	1173111	56.75

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21243	Lihuaxuella thermophila strain YIM 77831 16S ribosomal RNA gene, partial sequence	JX045707	1516		1173111
67770	Lihuaxuella thermophila strain YIM 77831 16S ribosomal RNA gene, partial sequence	JQ750619	1497		1173111

GC content

@ref	GC-content (mol%)	Method
21243	55.6	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lihuaxuella thermophila DSM 46701
NCBI: GCA_900110165

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	90.50	no
125439	motility	BacteriaNetⓘ	yes	76.60	no
125439	gram_stain	BacteriaNetⓘ	variable	89.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	70.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lihuaxuella thermophila strain DSM 46701
PATRIC: 1173111.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	73.53	no
125438	anaerobic	anaerobicⓘ	no	94.32	no
125438	spore-forming	spore-formingⓘ	yes	94.73	no
125438	aerobic	aerobicⓘ	yes	80.60	no
125438	thermophilic	thermophileⓘ	no	51.47	no
125438	flagellated	motile2+ⓘ	no	68.06	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Structure and stability of an apo thermophilic esterase that hydrolyzes polyhydroxybutyrate.	Thomas GM, Quirk S, Lieberman RL.	Acta Crystallogr D Struct Biol	10.1107/s2059798324009707	2024
	Identification and structural characterization of a novel acetyl xylan esterase from Aspergillus oryzae.	Yamada C, Kato T, Shiono Y, Koseki T, Fushinobu S.	FEBS J	10.1111/febs.17420	2025
Enzymology	A multidomain hydrolase from the thermophile Thermanaeromonas toyohensis degrades high molecular mass forms of polyhydroxybutyrate.	Quirk S, Thomas GM, Wallace LJ, Woody MJ, Peek ME, Lieberman RL.	Protein Sci	10.1002/pro.70223	2025
	Biodegradation of polyhydroxyalkanoates: current state and future prospects.	Paloyan A, Tadevosyan M, Ghevondyan D, Khoyetsyan L, Karapetyan M, Margaryan A, Antranikian G, Panosyan H.	Front Microbiol	10.3389/fmicb.2025.1542468	2025
Enzymology	Bioplastic degradation by a polyhydroxybutyrate depolymerase from a thermophilic soil bacterium.	Thomas GM, Quirk S, Huard DJE, Lieberman RL.	Protein Sci	10.1002/pro.4470	2022
Metabolism	Cellulonodin-2 and Lihuanodin: Lasso Peptides with an Aspartimide Post-Translational Modification.	Cao L, Beiser M, Koos JD, Orlova M, Elashal HE, Schroder HV, Link AJ.	J Am Chem Soc	10.1021/jacs.1c05017	2021
Metabolism	A Bacillus paralicheniformis Iron-Containing Urease Reduces Urea Concentrations in Rice Wine.	Liu Q, Chen Y, Yuan M, Du G, Chen J, Kang Z.	Appl Environ Microbiol	10.1128/aem.01258-17	2017
Phylogeny	Thermoactinomyces guangxiensis sp. nov., a thermophilic actinomycete isolated from mushroom compost.	Wu H, Liu B, Pan S	Int J Syst Evol Microbiol	10.1099/ijs.0.000342	2015
Phylogeny	Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China.	Yu TT, Zhang BH, Yao JC, Tang SK, Zhou EM, Yin YR, Wei DQ, Ming H, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-012-9771-6	2012

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21243	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46701
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lihuaxuella thermophila (DSM 46701, CCTCC AA 2011024, JCM 18059)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Observation

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900110165

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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