Name: Rhizobium leucaenae DSM 26699
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24483

Type strain:

Species: Rhizobium leucaenae

Culture col. no.: DSM 26699, CECT 4844, CENA 183, CFN 299, CNPSo 141, IAM 14230, JCM 21088, LMG 9517, SEMIA 4083, UMR 1026, USDA 9039

Strain history: IAM 14230 <-- E. Martínez-Romero CFN 299.

NCBI tax ID(s): 1041147 (strain), 29450 (species)

SI-ID 4809

LMG 9517, CECT 4844, IAM 14230, JCM 21088

Section

Rhizobium leucaenae DSM 26699 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from common beans Phaseolus vulgaris.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Rhizobium*
	Species	**Rhizobium leucaenae**
	Full Scientific Name (LPSN)	Rhizobium leucaenae Ribeiro et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30296]	Gram stain	negative

[Ref.: #30296]	Cell shape	rod-shaped

[Ref.: #21029]

Incubation period

3-7 days

[Ref.: #30296]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21029]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #21029]

Culture medium growth

[Ref.: #21029]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21029]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21029]	growth	28 °C
[Ref.: #30296]	growth	25-28 °C
[Ref.: #30296]	optimum	26.5 °C
[Ref.: #67770]	growth	25 °C

[Ref.: #21029]	Temperature range	mesophilic
[Ref.: #30296]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30296]			optimum	6
[Ref.: #30296]			growth	04-07

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30296]

Oxygen tolerance

aerobe

[Ref.: #30296]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30296]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30296]	cellobiose ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #30296]	fructose ChEBI	+	carbon source
[Ref.: #30296]	fucose ChEBI	+	carbon source
[Ref.: #30296]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30296]	gluconate ChEBI	+	carbon source
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30296]	glucose ChEBI	+	carbon source
[Ref.: #30296]	glycerol ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30296]	L-arabitol ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30296]	maltose ChEBI	+	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30296]	mannitol ChEBI	+	carbon source
[Ref.: #30296]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #30296]	ribose ChEBI	+	carbon source
[Ref.: #30296]	sucrose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #30296]	xylitol ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21029]	23953	-	-	-	-	+/-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21029]	Sample type/isolated from	common beans Phaseolus vulgaris
[Ref.: #21029]	Host species	Phaseolus vulgaris
[Ref.: #21029]	Country	Brazil
[Ref.: #21029]	Country ISO 3 Code	BRA
[Ref.: #21029]	Continent	Middle and South America

[Ref.: #67770]	Sample type/isolated from	Phaseolus vulgaris
[Ref.: #67770]	Host species	Phaseolus vulgaris
[Ref.: #67770]	Country	Brazil
[Ref.: #67770]	Country ISO 3 Code	BRA
[Ref.: #67770]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X67234 (>99% sequence identity) for Rhizobiaceae from Microbeatlas

Aquatic Samples	403
Soil Samples	3171
Animal Samples	1252
Plant Samples	1013
Total Samples	5839

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21029]	Rhizobium tropici subgroup IIA partial 16S rRNA gene, strain LMG 9517	X67234	1505	ENA	1041147 tax ID

[Ref.: #21029]

GC-content

64.1 mol%

Availability in culture collections External links

[Ref.: #21029]

Culture collection no.

DSM 26699, CECT 4844, CENA 183, CFN 299, CNPSo 141, IAM 14230, JCM 21088, LMG 9517, SEMIA 4083, UMR 1026, USDA 9039

[Ref.: #88344]

SI-ID 4809

Literature:

	Title	Authors	Journal	DOI	Year
Stress	Genome of Rhizobium leucaenae strains CFN 299(T) and CPAO 29.8: searching for genes related to a successful symbiotic performance under stressful conditions.	Ormeno-Orrillo E, Gomes DF, Del Cerro P, Vasconcelos AT, Canchaya C, Almeida LG, Mercante FM, Ollero FJ, Megias M, Hungria M	BMC Genomics	10.1186/s12864-016-2859-z	2016	*
Genetics	Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.).	Ormeno-Orrillo E, Menna P, Almeida LG, Ollero FJ, Nicolas MF, Pains Rodrigues E, Shigueyoshi Nakatani A, Silva Batista JS, Oliveira Chueire LM, Souza RC, Ribeiro Vasconcelos AT, Megias M, Hungria M, Martinez-Romero E	BMC Genomics	10.1186/1471-2164-13-735	2012	*
Phylogeny	Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov.	Ribeiro RA, Rogel MA, Lopez-Lopez A, Ormeno-Orrillo E, Barcellos FG, Martinez J, Thompson FL, Martinez-Romero E, Hungria M	Int J Syst Evol Microbiol	10.1099/ijs.0.032912-0	2011	*
	Exo-Metabolites of Phaseolus vulgaris-Nodulating Rhizobial Strains.	Montes-Grajales D, Esturau-Escofet N, Esquivel B, Martinez-Romero E	Metabolites	10.3390/metabo9060105	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21029

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26699

#30296

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26637 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#88344

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID4809.1 )

#26637

IJSEM 1179 2012 ( DOI 10.1099/ijs.0.032912-0 , PubMed 21742822 )

* These data were automatically processed and therefore are not curated

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