Pseudomonas entomophila (DSM 28517, CCUG 61470, CECT 7985)

Name: Pseudomonas entomophila (DSM 28517, CCUG 61470, CECT 7985)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24436

Type strain

Strain Designation L48

Culture col. no. DSM 28517 CCUG 61470 CECT 7985 L48 STH00794

NCBI tax ID(s) 312306

Special Collections DSMZ CCUG JMRC

History DSMZ <- CECT <- B. Lemaitre, Ecole Polytechnique Fédérale de Lausanne, Switzerland <- N. Vodovar

Links

Pseudomonas entomophila L48 is an aerobe animal pathogen that was isolated from Drosophila melanogaster.

aerobe animal pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 379920

CECT 7985,CCUG 61470,LMG 28944,DSM 28517

@ref 20215

Last LPSN update 2026-05-29 08:30:37

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas entomophila

Full scientific name Pseudomonas entomophila Mulet et al. 2012

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		90.876
125438		99	negative

Multimedia

@ref:	21139
multimedia content:	DSM_28517.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_28517.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21139	REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)		Medium recipe at MediaDive	Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
21139	positive	growth	28
62634	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
62634	aerobe
125438	aerobe	92.384
125439	facultative anaerobe	90.322

Spore formation

@ref	Spore formation	Confidence
125439		97.679

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	+	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	+	3.5.3.6	from API 20NE
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

Fatty acid profile

Metadata FA analysis

@ref

62634

Fatty acid	Percentage	ECL
C16:0	25.1	16
C16:1 ω7c	14.7	15.819
C17:0 CYCLO	13.1	16.888
C18:1 ω7c /12t/9t	12.2	17.824
C10:0 3OH	9.4	11.423
C12:0 2OH	6.8	13.178
C12:0 3OH	5	13.455
Unidentified	4.4	10.651
Unidentified	1.6	10.473
C12:0	1.5	12
C16:1 ω7c DMA	1.4	16.288
C18:2 ω6,9c/C18:0 ANTE	1.3	17.724
Unidentified	1.1	9.983
C18:1 ω9c	0.9	17.769
Unidentified	0.7	11.196
C15:0	0.5	15
Unidentified	0.4	9.395

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
21139	-	-	-	+	-	+	+	-	+	-	+	+	+	-	+	+	-	+	+	+	+
21139	-	-	-	+	-	-	-	-	+	-	+	+	+	-	+	+	-	+	+	+	+
21139	-	-	-	+	-	-	+	-	+	-	+	+	+	-	+	+	-	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Sampling date
21139	Drosophila melanogaster	Drosophila melanogaster	Calvaire (Guadeloupe)	France	FRA	Europe
62634	Drosophila		Guadeloupe, Calvaire	France	FRA	Europe	2000-02-01

Taxonmaps

69479

Global distribution of 16S sequence AY907566 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	543
Soil Samples	368
Animal Samples	799
Plant Samples	423
Total Samples	2133

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
21139		1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2610v1 assembly for Pseudomonas entomophila L48	complete	GCA_000026105	384676.9	637000219	384676	98.42

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21139	Pseudomonas entomophila strain L48 16S ribosomal RNA gene, partial sequence	AY907566	1515		384676

GC content

21139

GC-content (mol%)60

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2610v1 assembly for Pseudomonas entomophila L48
BV-BRC: 384676.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	90.32	no
125439	gram_stain	BacteriaNetⓘ	negative	58.33	no
125439	spore_formation	BacteriaNetⓘ	no	97.68	no
125439	motility	BacteriaNetⓘ	yes	67.04	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas entomophila L48 L48
NCBI: GCA_000026105.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.00	no
125438	anaerobic	anaerobicⓘ	no	98.95	yes
125438	aerobic	aerobicⓘ	yes	92.38	yes
125438	spore-forming	spore-formingⓘ	no	85.34	no
125438	thermophilic	thermophileⓘ	no	98.00	yes
125438	flagellated	motile2+ⓘ	yes	90.88	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Probiotic Enterococcus mundtii Isolate Protects the Model Insect Tribolium castaneum against Bacillus thuringiensis.	Grau T, Vilcinskas A, Joop G.	Front Microbiol	10.3389/fmicb.2017.01261	2017
Metabolism	Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis.	Pacheco I, Diaz-Sanchez S, Contreras M, Villar M, Cabezas-Cruz A, Gortazar C, de la Fuente J.	Pharmaceuticals (Basel)	10.3390/ph14070635	2021
	Bactericidal Effect of Pseudomonas oryziphila sp. nov., a Novel Pseudomonas Species Against Xanthomonas oryzae Reduces Disease Severity of Bacterial Leaf Streak of Rice.	Yang R, Li S, Li Y, Yan Y, Fang Y, Zou L, Chen G.	Front Microbiol	10.3389/fmicb.2021.759536	2021
Phylogeny	Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.	Mulet M, Gomila M, Lemaitre B, Lalucat J, Garcia-Valdes E	Syst Appl Microbiol	10.1016/j.syapm.2011.12.003	2012

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#21139	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28517
#62634	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61470
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas entomophila (DSM 28517, CCUG 61470, CECT 7985)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000026105

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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