Name: Pseudomonas entomophila L48
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24436

Type strain:

Species: Pseudomonas entomophila

Strain Designation: L48

Culture col. no.: DSM 28517, CCUG 61470, CECT 7985

Strain history: <- CECT <- B. Lemaitre, Ecole Polytechnique Fédérale de Lausanne, Switzerland <- N. Vodovar

NCBI tax ID(s): 312306 (species)

SI-ID 379920

CECT 7985, CCUG 61470, LMG 28944, DSM 28517

Section

Pseudomonas entomophila L48 is an aerobe, mesophilic animal pathogen that was isolated from Drosophila melanogaster.

aerobe
animal pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas entomophila**
	Full Scientific Name (LPSN)	Pseudomonas entomophila Mulet et al. 2012

Information on morphological and physiological properties Morphology

Multimedia content

[Ref.: #21139]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21139]

Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

[Ref.: #21139]

Culture medium growth

[Ref.: #21139]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21139]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #21139]			growth	28 °C
[Ref.: #62634]			growth	30 °C

[Ref.: #21139]	Temperature range	mesophilic
[Ref.: #62634]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #62634]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #62634]

Fatty acid	Percentage	ECL
C16:0	25.1	16
C16:1 ω7c	14.7	15.819
C17:0 CYCLO	13.1	16.888
C18:1 ω7c /12t/9t	12.2	17.824
C10:0 3OH	9.4	11.423
C12:0 2OH	6.8	13.178
C12:0 3OH	5	13.455
Unidentified	4.4	10.651
Unidentified	1.6	10.473
C12:0	1.5	12
C16:1 ω7c DMA	1.4	16.288
C18:2 ω6,9c/C18:0 ANTE	1.3	17.724
Unidentified	1.1	9.983
C18:1 ω9c	0.9	17.769
Unidentified	0.7	11.196
C15:0	0.5	15
Unidentified	0.4	9.395

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21139]	24989	-	-	-	+	-	+	+	-	+	-	+	+	+	-	+	+	-	+	+	+	+
[Ref.: #21139]	23284	-	-	-	+	-	-	-	-	+	-	+	+	+	-	+	+	-	+	+	+	+
[Ref.: #21139]	23157	-	-	-	+	-	-	+	-	+	-	+	+	+	-	+	+	-	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21139]	Sample type/isolated from	Drosophila melanogaster
[Ref.: #21139]	Host species	Drosophila melanogaster
[Ref.: #21139]	Geographic location (country and/or sea, region)	Calvaire (Guadeloupe)
[Ref.: #21139]	Country	France
[Ref.: #21139]	Country ISO 3 Code	FRA
[Ref.: #21139]	Continent	Europe

[Ref.: #62634]	Sample type/isolated from	Drosophila
[Ref.: #62634]	Sampling date	2000-02-01
[Ref.: #62634]	Geographic location (country and/or sea, region)	Guadeloupe, Calvaire
[Ref.: #62634]	Country	France
[Ref.: #62634]	Country ISO 3 Code	FRA
[Ref.: #62634]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host

#Arthropoda

#Insecta

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY907566 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	543
Soil Samples	368
Animal Samples	799
Plant Samples	423
Total Samples	2133

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21139]	Pathogenicity (animal)	yes
[Ref.: #21139]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21139]	Pseudomonas entomophila strain L48 16S ribosomal RNA gene, partial sequence	AY907566	1515	ENA	384676 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas entomophila L48	GCA_000026105	complete	GenBank	384676 tax ID	*
[Ref.: #66792]	Pseudomonas entomophila L48	384676.9	complete	PATRIC	384676 tax ID	*
[Ref.: #66792]	Pseudomonas entomophila L48	637000219	complete	JGI IMG/M	384676 tax ID	*

[Ref.: #21139]

GC-content

60 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas entomophila L48 L48 NCBI: GCA_000026105.1
[Ref.: #69480]	motile	yes	90.577	no
[Ref.: #69480]	flagellated	yes	84.067	no
[Ref.: #69480]	gram-positive	no	98.253	no
[Ref.: #69480]	anaerobic	no	98.834	yes
[Ref.: #69480]	aerobic	yes	96.038	yes
[Ref.: #69480]	halophile	no	86.739	no
[Ref.: #69480]	spore-forming	no	94.598	no
[Ref.: #69480]	glucose-util	yes	95.378	yes
[Ref.: #69480]	thermophile	no	99.847	yes
[Ref.: #69480]	glucose-ferment	no	88.175	yes

Availability in culture collections External links

[Ref.: #21139]

Culture collection no.

DSM 28517, CCUG 61470, CECT 7985

[Ref.: #88315]

SI-ID 379920

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Taxonomic characterisation of Pseudomonas strain L48 and formal proposal of Pseudomonas entomophila sp. nov.	Mulet M, Gomila M, Lemaitre B, Lalucat J, Garcia-Valdes E	Syst Appl Microbiol	10.1016/j.syapm.2011.12.003	2012	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21139

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28517

#62634 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61470

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#88315

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID379920.1 )

* These data were automatically processed and therefore are not curated

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Pseudomonas entomophila L48 L48

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