Name: Methylophaga nitratireducenticrescens DSM 25689
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24314

Type strain:

Species: Methylophaga nitratireducenticrescens

Culture col. no.: DSM 25689, ATCC BAA-2433, JAM 1

Strain history: <- R. Villemur, INRS-IAF; JAM1 <- J. Auclair, IAF

NCBI tax ID(s): 754476 (species)

SI-ID 403836

DSM 25689

Section

Methylophaga nitratireducenticrescens DSM 25689 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from biofilm of a methanol-fed denitrification system treating seawater.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Beggiatoales*
	Family	*Piscirickettsiaceae*
	Genus	*Methylophaga*
	Species	**Methylophaga nitratireducenticrescens**
	Full Scientific Name (LPSN)	Methylophaga nitratireducenticrescens Villeneuve et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30798]	Gram stain	negative

[Ref.: #30798]	Cell length	1.5 µm

[Ref.: #30798]	Cell width	0.6 µm

[Ref.: #30798]	Cell shape	rod-shaped

[Ref.: #30798]	Motility	yes

[Ref.: #20653]

Incubation period

3-7 days

[Ref.: #30798]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20653]

Culture medium

METHYLOPHAGA MEDIUM 1403 (DSMZ Medium 1337)

[Ref.: #20653]

Culture medium growth

[Ref.: #20653]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20653]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #20653]	growth	28 °C
[Ref.: #30798]	growth	15-37 °C
[Ref.: #30798]	optimum	30 °C

[Ref.: #20653]	Temperature range	mesophilic
[Ref.: #30798]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30798]			optimum	8
[Ref.: #30798]			growth	06-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30798]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30798]		NaCl		growth	0.5-8	%
[Ref.: #30798]		NaCl		optimum	3	%

[Ref.: #30798]

Observation

aggregates in clumps

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30798]		nitrate ChEBI	+	reduction

	Enzyme	Enzyme activity	EC number
[Ref.: #30798]	alkaline phosphatase	+	3.1.3.1
[Ref.: #30798]	catalase	+	1.11.1.6
[Ref.: #30798]	cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20653]	Sample type/isolated from	biofilm of a methanol-fed denitrification system treating seawater
[Ref.: #20653]	Geographic location (country and/or sea, region)	Montréal, Biodome
[Ref.: #20653]	Country	Canada
[Ref.: #20653]	Country ISO 3 Code	CAN
[Ref.: #20653]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Machines and devices
#Environmental	#Aquatic	#Marine
#Environmental	#Biofilm	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GU567814 (>99% sequence identity) for Methylophaga nitratireducenticrescens subclade from Microbeatlas

Aquatic Samples	89
Soil Samples	80
Animal Samples	44
Plant Samples	7
Total Samples	220

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20653]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20653]	Methylophaga nitratireducenticrescens strain JAM1 16S ribosomal RNA gene, partial sequence	GU567814	1453	ENA	754476 tax ID

[Ref.: #30798]

GC-content

44.7 mol%

Availability in culture collections External links

[Ref.: #20653]

Culture collection no.

DSM 25689, ATCC BAA-2433, JAM 1

[Ref.: #88247]

SI-ID 403836

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylophaga pinxianii sp. nov., isolated from the Mariana Trench.	Wei TT, Zhang RY, Quan ZX	Int J Syst Evol Microbiol	10.1099/ijsem.0.005414	2022	*
Metabolism	Denitrifying metabolism of the methylotrophic marine bacterium Methylophaga nitratireducenticrescens strain JAM1.	Mauffrey F, Cucaita A, Constant P, Villemur R	PeerJ	10.7717/peerj.4098	2017	*
Enzymology	Importance of the Two Dissimilatory (Nar) Nitrate Reductases in the Growth and Nitrate Reduction of the Methylotrophic Marine Bacterium Methylophaga nitratireducenticrescens JAM1.	Mauffrey F, Martineau C, Villemur R	Front Microbiol	10.3389/fmicb.2015.01475	2015	*
Phylogeny	Methylophaga nitratireducenticrescens sp. nov. and Methylophaga frappieri sp. nov., isolated from the biofilm of the methanol-fed denitrification system treating the seawater at the Montreal Biodome.	Villeneuve C, Martineau C, Mauffrey F, Villemur R	Int J Syst Evol Microbiol	10.1099/ijs.0.044545-0	2012	*
	Co-culturing Hyphomicrobium nitrativorans strain NL23 and Methylophaga nitratireducenticrescens strain JAM1 allows sustainable denitrifying activities under marine conditions.	Cucaita A, Piochon M, Villemur R	PeerJ	10.7717/peerj.12424	2021	*
	Dynamics of a methanol-fed marine denitrifying biofilm: 1-Impact of environmental changes on the denitrification and the co-occurrence of Methylophaga nitratireducenticrescens and Hyphomicrobium nitrativorans.	Payette G, Geoffroy V, Martineau C, Villemur R	PeerJ	10.7717/peerj.7497	2019	*
	Strain-level genetic diversity of Methylophaga nitratireducenticrescens confers plasticity to denitrification capacity in a methylotrophic marine denitrifying biofilm.	Geoffroy V, Payette G, Mauffrey F, Lestin L, Constant P, Villemur R	PeerJ	10.7717/peerj.4679	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20653

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25689

#30798

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27129 (see below)

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#88247

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403836.1 )

#27129

IJSEM 2216 2013 ( DOI 10.1099/ijs.0.044545-0 , PubMed 23148104 )

* These data were automatically processed and therefore are not curated

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