Name: Paenibacillus thermoaerophilus TC22-2b
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24287

Type strain:

Species: Paenibacillus thermoaerophilus

Strain Designation: TC22-2b

Culture col. no.: DSM 26310, JCM 18657

Strain history: N. Kurosawa TC22-2b.

NCBI tax ID(s): 1215385 (species)

SI-ID 402790

DSM 26310

Section

Paenibacillus thermoaerophilus TC22-2b is an aerobe, spore-forming, thermophilic bacterium that was isolated from compost.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus thermoaerophilus**
	Full Scientific Name (LPSN)	Paenibacillus thermoaerophilus Ueda et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30943]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #30943]	Cell length	4 µm

[Ref.: #30943]	Cell width	0.7 µm

[Ref.: #30943]	Cell shape	rod-shaped

[Ref.: #30943]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %94.982

[Ref.: #30943]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20711]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #20711]

Culture medium growth

[Ref.: #20711]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20711]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #20711]	growth	50 °C
[Ref.: #30943]	growth	25-58 °C
[Ref.: #30943]	optimum	52.5 °C
[Ref.: #67770]	growth	50 °C

[Ref.: #20711]	Temperature range	thermophilic
[Ref.: #30943]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #30943]			optimum	7.5
[Ref.: #30943]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30943]

Oxygen tolerance

aerobe

[Ref.: #30943]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30943]		NaCl		growth	0-3.5	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30943]	glucose ChEBI	+	carbon source
[Ref.: #30943]	mannitol ChEBI	+	carbon source
[Ref.: #30943]	mannose ChEBI	+	carbon source
[Ref.: #30943]	N-acetylglucosamine ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #30943]		catalase	+	1.11.1.6
[Ref.: #30943]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20711]	Sample type/isolated from	compost
[Ref.: #20711]	Geographic location (country and/or sea, region)	Tochigi Prefecture
[Ref.: #20711]	Country	Japan
[Ref.: #20711]	Country ISO 3 Code	JPN
[Ref.: #20711]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Compost in Tochigi
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered

#Biodegradation

#Composting

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB738878 (>99% sequence identity) for Paenibacillus thermoaerophilus subclade from Microbeatlas

Aquatic Samples	149
Soil Samples	419
Animal Samples	130
Plant Samples	49
Total Samples	747

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20711]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20711]	Paenibacillus thermoaerophilus gene for 16S rRNA, partial sequence	AB738878	1474	ENA	1215385 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Paenibacillus thermoaerophilus DSM 26310	GCA_005938195	scaffold	GenBank	1215385 tax ID
[Ref.: #66792]	Paenibacillus thermoaerophilus strain DSM 26310	1215385.3	wgs	PATRIC	1215385 tax ID	*

[Ref.: #20711]	GC-content	59.1 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30943]	GC-content	59.1 mol%
[Ref.: #67770]	GC-content	58.7-59.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paenibacillus thermoaerophilus DSM 26310 NCBI: GCA_005938195.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus thermoaerophilus strain DSM 26310 PATRIC: 1215385.3
[Ref.: #69480]	motile	yes	87.829	yes
[Ref.: #69480]	gram-positive	yes	86.916	yes
[Ref.: #69480]	anaerobic	no	98.629	no
[Ref.: #69480]	aerobic	yes	91.592	yes
[Ref.: #69480]	halophile	no	94.971	no
[Ref.: #69480]	spore-forming	yes	96.31	yes
[Ref.: #69480]	thermophile	no	59.631	yes
[Ref.: #69480]	glucose-util	yes	88.251	no
[Ref.: #69480]	flagellated	yes	84.851	no
[Ref.: #69480]	glucose-ferment	no	88.738	no

Availability in culture collections External links

[Ref.: #20711]

Culture collection no.

DSM 26310, JCM 18657

[Ref.: #88225]

SI-ID 402790

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus thermoaerophilus sp. nov., a moderately thermophilic bacterium isolated from compost.	Ueda J, Yamamoto S, Kurosawa N	Int J Syst Evol Microbiol	10.1099/ijs.0.048090-0	2013	*
Phylogeny	Description of Paenibacillus antri sp. nov. and Paenibacillus mesophilus sp. nov., isolated from cave soil.	Narsing Rao MP, Dong ZY, Kan Y, Zhang K, Fang BZ, Xiao M, Kang YQ, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003870	2020	*
Enzymology	Characterization of an extracellular thermophilic chitinase from Paenibacillus thermoaerophilus strain TC22-2b isolated from compost.	Ueda J, Kurosawa N	World J Microbiol Biotechnol	10.1007/s11274-014-1754-5	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20711

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26310

#30943

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27273 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88225

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402790.1 )

#27273

IJSEM 3330 2013 ( DOI 10.1099/ijs.0.048090-0 , PubMed 23504971 )

* These data were automatically processed and therefore are not curated

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Paenibacillus thermoaerophilus DSM 26310

Paenibacillus thermoaerophilus strain DSM 26310

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