Name: Mycobacterium bourgelatii 02-5273-A84
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24238

Type strain:

Species: Mycobacterium bourgelatii

Strain Designation: 02-5273-A84, MLB-A84, MLB-A84 (5273)

Culture col. no.: DSM 45746, CIP 110557, JCM 30725

Strain history: CIP <- 2012, B. Lamy, Montpellier Hosp., Montpellier, France: strain MLB-A84 (5273)

NCBI tax ID(s): 1273442 (species)

SI-ID 391579

CIP 110557, DSM 45746

Section

Mycobacterium bourgelatii 02-5273-A84 is a mesophilic, Gram-positive, rod-shaped animal pathogen that was isolated from bovine lymph nodes.

Gram-positive
rod-shaped
animal pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium bourgelatii**
	Full Scientific Name (LPSN)	Mycobacterium bourgelatii Guérin-Faublée et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #31096]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31096]	Cell shape	rod-shaped
[Ref.: #116901]	Cell shape	rod-shaped

[Ref.: #116901]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91.311

[Ref.: #31096]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #41221]

Culture medium

MEDIUM 55 - for Mycobacterium

[Ref.: #41221]

Culture medium growth

[Ref.: #20673]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #20673]

Culture medium growth

[Ref.: #20673]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20673]

Culture medium composition

expand / minimize

[Ref.: #116901]

Culture medium

CIP Medium 55

[Ref.: #116901]

Culture medium growth

[Ref.: #116901]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #20673]	growth	37 °C
[Ref.: #31096]	growth	20-42 °C
[Ref.: #31096]	optimum	42 °C
[Ref.: #41221]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #20673]	Temperature range	mesophilic
[Ref.: #31096]	Temperature range	thermophilic
[Ref.: #41221]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.748

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #31096]	arylsulfatase	+	3.1.6.1
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31096]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #20673]	24776	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+/-	-	+/-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20673]	14115	-	-	+/-	+	+/-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20673]	Sample type/isolated from	bovine lymph nodes
[Ref.: #20673]	Geographic location (country and/or sea, region)	Eure
[Ref.: #20673]	Country	France
[Ref.: #20673]	Country ISO 3 Code	FRA
[Ref.: #20673]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Bovine lymph nodes
[Ref.: #67770]	Country	France
[Ref.: #67770]	Country ISO 3 Code	FRA
[Ref.: #67770]	Continent	Europe

[Ref.: #116901]	Sample type/isolated from	Animal, Bovine
[Ref.: #116901]	Geographic location (country and/or sea, region)	Eure
[Ref.: #116901]	Country	France
[Ref.: #116901]	Country ISO 3 Code	FRA
[Ref.: #116901]	Continent	Europe
[Ref.: #116901]	Isolation date	2002
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Organ	#Lymph node

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20673]	Pathogenicity (animal)	yes, in single cases
[Ref.: #20673]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116901]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20673]	Mycobacterium bourgelatii partial 16S rRNA gene, isolate 02-5273-A84	HF674384	1284	ENA	1273442 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycobacterium bourgelatii JCM 30725	GCA_010723575	contig	GenBank	1273442 tax ID	*
[Ref.: #66792]	Mycobacterium bourgelatii strain JCM 30725	1273442.8	wgs	PATRIC	1273442 tax ID	*
[Ref.: #66792]	Mycobacterium bourgelatii DSM 45746	GCA_025821955	scaffold	GenBank	1273442 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycobacterium bourgelatii strain JCM 30725 PATRIC: 1273442.8
[Ref.: #69481]	spore-forming	no	74	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium bourgelatii strain JCM 30725 PATRIC: 1273442.8
[Ref.: #69480]	motile	no	94.979	no
[Ref.: #69480]	gram-positive	yes	89.666	yes
[Ref.: #69480]	anaerobic	no	98.868	no
[Ref.: #69480]	aerobic	yes	90.433	no
[Ref.: #69480]	halophile	no	90.961	no
[Ref.: #69480]	spore-forming	no	78.046	no
[Ref.: #69480]	glucose-util	yes	62.201	yes
[Ref.: #69480]	flagellated	no	98.937	no
[Ref.: #69480]	thermophile	no	98.121	no
[Ref.: #69480]	glucose-ferment	no	92.881	yes

Availability in culture collections External links

[Ref.: #20673]

Culture collection no.

DSM 45746, CIP 110557, JCM 30725

[Ref.: #88181]

SI-ID 391579

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mycobacterium bourgelatii sp. nov., a rapidly growing, non-chromogenic species isolated from the lymph nodes of cattle.	Guerin-Faublee V, Flandrois JP, Pichat C, Boschiroli ML, Lamy B	Int J Syst Evol Microbiol	10.1099/ijs.0.051979-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20673

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45746

#31096

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27425 (see below)

#41221 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88181

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID391579.1 )

#116901 Collection of Institut Pasteur ; Curators of the CIP; CIP 110557

#27425

IJSEM 4669 2013 ( DOI 10.1099/ijs.0.051979-0 , PubMed 23990648 )

* These data were automatically processed and therefore are not curated

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Mycobacterium bourgelatii strain JCM 30725

Mycobacterium bourgelatii strain JCM 30725

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