Mycobacterium lentiflavum (DSM 46733, ATCC 51985, CCUG 42422)

Name: Mycobacterium lentiflavum (DSM 46733, ATCC 51985, CCUG 42422)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24232

Type strain

Strain Designation 2186/92

Culture col. no. DSM 46733 ATCC 51985 CCUG 42422 CIP 105465 JCM 13390 3 more

NCBI tax ID(s) 141349

Special Collections DSMZ CCUG JCM CIP

Links

Mycobacterium lentiflavum 2186/92 is a mesophilic, rod-shaped prokaryote that was isolated from human biopsy from vertebrae, spondylodiscitis.

rod-shaped mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 45334

ATCC 51985,CCUG 37518,DSM 44195,CIP 105465,DSM 44418,CCUG 42422,CCUG 42559,JCM 13390,DSM 46733

@ref 20215

Last LPSN update 2025-12-16 09:52:48

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium lentiflavum

Full scientific name Mycobacterium lentiflavum Springer et al. 1996

BacDive ID	Other strains from **Mycobacterium lentiflavum** (7)	Type strain
8456	M. lentiflavum Hannover 2228/94, TBC 1472/94, DSM 44419
8457	M. lentiflavum Hannover 4351/92, TBC 988/93, DSM 44420
8458	M. lentiflavum Hannover 1455/94, TBC 745/94, DSM 44421
8459	M. lentiflavum Hannover 1577/93, TBC 1029/93, DSM 44422
8460	M. lentiflavum Böttger Hannover 205621/97, TBC 268/97, ...
150815	M. lentiflavum CCUG 42423, ATCC 51987
152888	M. lentiflavum CCUG 47901

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	positive	90.958
120581

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
20667	MIDDLEBROOK MEDIUM (DSMZ Medium 645)		Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
120581	CIP Medium 55		Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
20667	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
120581	positive	growth	22-37
120581	negative	growth	10
120581	negative	growth	45

Physiology and metabolism

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120581	606565 ChEBI	hippurate	-	hydrolysis
120581	17632 ChEBI	nitrate	-	reduction
120581	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120581	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
120581	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120581	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120581	gamma-glutamyltransferase	-	2.3.2.2
120581	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120581	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
120581	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120581	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Infection	#Inflammation
#Infection	#Patient
#Host	#Human

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
20667	human biopsy from vertebrae, spondylodiscitis	Hannover, Medical School (MHH)	Germany	DEU	Europe
67770	Patient with spondylodiscitis
120581	Human, Spondylodiscitis

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
20667	2	Risk group (German classification)
120581	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2237489v2 assembly for Mycobacterium lentiflavum ATCC 51985	complete	GCA_022374895	141349.8	8111301230	141349	97.97

Genome browser: GCA_022374895

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Mycobacterium lentiflavum 16S ribosomal RNA gene, partial sequence; and 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF317658	413	141349
20218	Mycobacterium lentiflavum 16S ribosomal RNA gene, partial sequence	AF480583	1452	141349
67770	Mycobacterium sp. 16S rRNA gene	X80769	1421	1785
124043	Mycobacterium lentiflavum strain CIP 105465 16S ribosomal RNA gene, partial sequence.	AF547942	542	141349

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycobacterium lentiflavum ATCC 51985
NCBI: GCA_022374895.2

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.96	no
125438	anaerobic	anaerobicⓘ	no	96.87	no
125438	aerobic	aerobicⓘ	yes	77.44	no
125438	spore-forming	spore-formingⓘ	no	62.16	no
125438	thermophilic	thermophileⓘ	no	95.39	yes
125438	flagellated	motile2+ⓘ	no	89.50	no

Literature

Title	Authors	Journal	DOI	Year
Complete Genome Sequences of 14 Nontuberculous Mycobacteria Type Strains.	Igarashi Y, Osugi A, Murase Y, Chikamatsu K, Shimomura Y, Hosoya M, Aono A, Morishige Y, Yamada H, Takaki A, Mitarai S.	Microbiol Resour Announc	10.1128/mra.01214-22	2023
Clinical Evaluation of Nontuberculous Mycobacteria (NTM) Elite Agar, a New Medium for the Isolation of NTM: a Multicenter Study.	Broncano-Lavado A, Barrado L, Lopez-Roa P, Cacho J, Domingo D, Hernandez S, Garcia-Martinez J, Millan MR, Perez-Cecilia E, Ruiz-Serrano MJ, Salso S, Simon M, Tato M, Toro C, Valverde-Canovas JF, Esteban J.	J Clin Microbiol	10.1128/jcm.00036-23	2023
Disseminated Mycobacterium lentiflavum infection in a human immunodeficiency virus-infected patient.	Niobe SN, Bebear CM, Clerc M, Pellegrin JL, Bebear C, Maugein J.	J Clin Microbiol	10.1128/jcm.39.5.2030-2032.2001	2001
Rapid identification of mycobacterial whole cells in solid and liquid culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry.	Lotz A, Ferroni A, Beretti JL, Dauphin B, Carbonnelle E, Guet-Revillet H, Veziris N, Heym B, Jarlier V, Gaillard JL, Pierre-Audigier C, Frapy E, Berche P, Nassif X, Bille E.	J Clin Microbiol	10.1128/jcm.01397-10	2010
Evaluation of the invader assay with the BACTEC MGIT 960 system for prompt isolation and identification of mycobacterial species from clinical specimens.	Ichimura S, Nagano M, Ito N, Shimojima M, Egashira T, Miyamoto C, Ohkusu K, Ezaki T.	J Clin Microbiol	10.1128/jcm.02289-06	2007
Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex.	Bouam A, Heidarieh P, Shahraki AH, Pourahmad F, Mirsaeidi M, Hashemzadeh M, Baptiste E, Armstrong N, Levasseur A, Robert C, Drancourt M.	Sci Rep	10.1038/s41598-018-22526-z	2018
Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov.	Springer B, Wu WK, Bodmer T, Haase G, Pfyffer GE, Kroppenstedt RM, Schroder KH, Emler S, Kilburn JO, Kirschner P, Telenti A, Coyle MB, Bottger EC.	J Clin Microbiol	10.1128/jcm.34.5.1100-1107.1996	1996

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#20667	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46733
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#120581	Collection of Institut Pasteur ; Curators of the CIP; CIP 105465
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium lentiflavum (DSM 46733, ATCC 51985, CCUG 42422)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_022374895

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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