Name: Pseudarthrobacter siccitolerans 4J27
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24196

Type strain:

Species: Pseudarthrobacter siccitolerans

Strain Designation: 4J27

Culture col. no.: DSM 28024, CECT 8257, LMG 27359, CIP 110704

Strain history: CIP <- 2014, LMG <- 2012, L. SantaCruz-Calvo, Granada Univ., Granada, Spain <- 2009, J. J. Narvaez-Reinaldo, Granada Univ., Granada, Spain: strain 4J27

NCBI tax ID(s): 861266 (species)

SI-ID 382574

LMG 27359, CECT 8257, DSM 28024, CIP 110704

Section

Pseudarthrobacter siccitolerans 4J27 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from Nerium oleander rhizosphere subjected to seasonal drought.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Pseudarthrobacter*
	Species	**Pseudarthrobacter siccitolerans**
	Full Scientific Name (LPSN)	Pseudarthrobacter siccitolerans (SantaCruz-Calvo et al. 2013) Busse 2016

Synonym

Arthrobacter siccitolerans

Information on morphological and physiological properties Morphology

[Ref.: #31127]	Gram stain	positive
[Ref.: #122601]	Gram stain	positive

[Ref.: #31127]	Cell shape	rod-shaped
[Ref.: #122601]	Cell shape	rod-shaped

[Ref.: #31127]	Motility	no
[Ref.: #122601]	Motility	no

[Ref.: #31127]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20321]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #20321]

Culture medium growth

[Ref.: #20321]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20321]

Culture medium composition

expand / minimize

[Ref.: #122601]

Culture medium

CIP Medium 72

[Ref.: #122601]

Culture medium growth

[Ref.: #122601]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #20321]	growth	28 °C
[Ref.: #31127]	growth	15-35 °C
[Ref.: #31127]	optimum	30 °C

[Ref.: #20321]	Temperature range	mesophilic
[Ref.: #31127]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31127]			optimum	7
[Ref.: #31127]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31127]

Oxygen tolerance

aerobe

[Ref.: #31127]

Ability of spore formation

[Ref.: #20321]

Name of produced compound

xeroprotectants

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31127]		NaCl		growth	0-4.64	%
[Ref.: #31127]		NaCl		optimum	1.16	%

[Ref.: #31127]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31127]	2-oxopentanoate ChEBI	+	carbon source
[Ref.: #31127]	acetate ChEBI	+	carbon source
[Ref.: #31127]	arabinose ChEBI	+	carbon source
[Ref.: #31127]	cellobiose ChEBI	+	carbon source
[Ref.: #31127]	dextrin ChEBI	+	carbon source
[Ref.: #31127]	esculin ChEBI	+	hydrolysis
[Ref.: #31127]	fructose ChEBI	+	carbon source
[Ref.: #31127]	galactose ChEBI	+	carbon source
[Ref.: #31127]	galacturonate ChEBI	+	carbon source
[Ref.: #31127]	glucose ChEBI	+	carbon source
[Ref.: #31127]	glutamate ChEBI	+	carbon source
[Ref.: #31127]	glycerol ChEBI	+	carbon source
[Ref.: #31127]	glycine ChEBI	+	carbon source
[Ref.: #31127]	inosine ChEBI	+	carbon source
[Ref.: #31127]	L-alaninamide ChEBI	+	carbon source
[Ref.: #31127]	lactate ChEBI	+	carbon source
[Ref.: #31127]	malate ChEBI	+	carbon source
[Ref.: #31127]	maltose ChEBI	+	carbon source
[Ref.: #31127]	mannitol ChEBI	+	carbon source
[Ref.: #31127]	mannose ChEBI	+	carbon source
[Ref.: #31127]	melibiose ChEBI	+	carbon source
[Ref.: #31127]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #31127]	putrescine ChEBI	+	carbon source
[Ref.: #31127]	pyruvate ChEBI	+	carbon source
[Ref.: #31127]	raffinose ChEBI	+	carbon source
[Ref.: #31127]	rhamnose ChEBI	+	carbon source
[Ref.: #31127]	ribose ChEBI	+	carbon source
[Ref.: #31127]	salicin ChEBI	+	carbon source
[Ref.: #31127]	sorbitol ChEBI	+	carbon source
[Ref.: #31127]	sucrose ChEBI	+	carbon source
[Ref.: #31127]	thymidine ChEBI	+	carbon source
[Ref.: #31127]	trehalose ChEBI	+	carbon source
[Ref.: #31127]	uridine ChEBI	+	carbon source
[Ref.: #31127]	xylitol ChEBI	+	carbon source
[Ref.: #31127]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #31127]		indole ChEBI	yes

	Physiological tests	Metabolite	Indole test
[Ref.: #31127]		indole ChEBI	+

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #31127]		catalase	+	1.11.1.6
[Ref.: #31127]		pyrazinamidase	+	3.5.1.B15

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20321]	Sample type/isolated from	Nerium oleander rhizosphere subjected to seasonal drought
[Ref.: #20321]	Host species	Nerium oleander
[Ref.: #20321]	Geographic location (country and/or sea, region)	Granada
[Ref.: #20321]	Country	Spain
[Ref.: #20321]	Country ISO 3 Code	ESP
[Ref.: #20321]	Continent	Europe

[Ref.: #122601]	Sample type/isolated from	Environment, Nerium oleander, rhizosphere
[Ref.: #122601]	Geographic location (country and/or sea, region)	Granada, Spain
[Ref.: #122601]	Country	Spain
[Ref.: #122601]	Country ISO 3 Code	ESP
[Ref.: #122601]	Continent	Europe
[Ref.: #122601]	Isolation date	2009
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Shrub (Scrub)
#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GU815139 (>99% sequence identity) for Pseudarthrobacter from Microbeatlas

Aquatic Samples	109
Soil Samples	822
Animal Samples	156
Plant Samples	439
Total Samples	1526

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20321]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122601]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20321]	Arthrobacter siccitolerans strain 4J27 16S ribosomal RNA gene, partial sequence	GU815139	1420	ENA	861266 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudarthrobacter siccitolerans 4J27	GCA_001046895	contig	GenBank	861266 tax ID	*
[Ref.: #66792]	Pseudarthrobacter siccitolerans 4J27	2648501281	draft	JGI IMG/M	861266 tax ID	*

[Ref.: #20321]	GC-content	65.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #31127]	GC-content	65.3 mol%

Availability in culture collections External links

[Ref.: #20321]

Culture collection no.

DSM 28024, CECT 8257, LMG 27359, CIP 110704

[Ref.: #88144]

SI-ID 382574

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Arthrobacter ginsengisoli sp. nov., isolated from soil of a ginseng field.	Siddiqi MZ, Kim YJ, Hoang VA, Siddiqi MH, Huq MA, Yang DC	Arch Microbiol	10.1007/s00203-014-1025-8	2014	*
Phylogeny	Arthrobacter siccitolerans sp. nov., a highly desiccation-tolerant, xeroprotectant-producing strain isolated from dry soil.	SantaCruz-Calvo L, Gonzalez-Lopez J, Manzanera M	Int J Syst Evol Microbiol	10.1099/ijs.0.052902-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20321

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28024

#31127

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27456 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#88144

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID382574.1 )

#122601 Collection of Institut Pasteur ; Curators of the CIP; CIP 110704

#27456

IJSEM 4174 2013 ( DOI 10.1099/ijs.0.052902-0 , PubMed 23771623 )

* These data were automatically processed and therefore are not curated

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