Name: Roseiflexus castenholzii HLO8
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 2416

Type strain:

Species: Roseiflexus castenholzii

Strain Designation: HLO8

Culture col. no.: DSM 13941, JCM 11240, NBRC 100045

Strain history: S. Hanada HLO8.

NCBI tax ID(s): 383372 (strain), 120962 (species)

SI-ID 49536

DSM 13941, JCM 11240, NBRC 100045

Special Collections

Section

Roseiflexus castenholzii HLO8 is an anaerobe, photoheterotroph, Gram-negative bacterium that was isolated from a bacterial mat in Nakabusa hot spring .

filament-shaped
Gram-negative
motile
anaerobe
photoheterotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Chloroflexota*
	Class	*Chloroflexia*
	Order	*Chloroflexales*
	Family	*Roseiflexaceae*
	Genus	*Roseiflexus*
	Species	**Roseiflexus castenholzii**
	Full Scientific Name (LPSN)	Roseiflexus castenholzii Hanada et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #23075]	Gram stain	negative

[Ref.: #23075]	Cell width	0.8-1.0 µm

[Ref.: #23075]	Cell shape	filament-shaped

[Ref.: #23075]	Motility	yes

[Ref.: #23075]	Colony color	red to reddish-brown
[Ref.: #23075]	Cultivation medium used	1.5% agar-containing PE plate

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5192]

Culture medium

CHLOROFLEXUS AGGREGANS MEDIUM (DSMZ Medium 87a)

[Ref.: #5192]

Culture medium growth

[Ref.: #5192]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5192]

Culture medium composition

expand / minimize

[Ref.: #23075]

Culture medium

1.5% agar-containing PE plate

[Ref.: #23075]

Culture medium growth

		Temperature
[Ref.: #5192]	growth	48 °C
[Ref.: #23075]	growth	45.0-55.0 °C
[Ref.: #23075]	optimum	50.0 °C
[Ref.: #67770]	growth	50 °C

	pH	Kind of pH		pH
[Ref.: #23075]			optimum	7.5-8.0
[Ref.: #23075]			growth	7.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5192]

Oxygen tolerance

anaerobe

[Ref.: #23075]	Nutrition type	photoheterotroph
[Ref.: #23075]	Nutrition type	chemoheterotroph

[Ref.: #67770]

Observation

quinones: MK-11

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23075]		yeast extract	+	carbon source
[Ref.: #23075]		yeast extract (0.01 %, w/v)	+	growth

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5192]	Sample type/isolated from	a bacterial mat in Nakabusa hot spring (40-60°C, pH 7.8-8.2)
[Ref.: #5192]	Geographic location (country and/or sea, region)	Nagano Pref.
[Ref.: #5192]	Country	Japan
[Ref.: #5192]	Country ISO 3 Code	JPN
[Ref.: #5192]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Bacterial mat in Nakabusa hot spring
[Ref.: #67770]	Geographic location (country and/or sea, region)	Nagano Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #23075]	Enrichment culture	PE medium
[Ref.: #23075]	Enrichment culture temperature	50.0 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Environmental	#Microbial community	#Microbial mat
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB041226 (>99% sequence identity) for Roseiflexus castenholzii subclade from Microbeatlas

Aquatic Samples	615
Soil Samples	508
Animal Samples	457
Plant Samples	35
Total Samples	1615

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5192]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5192]	Roseiflexus castenholzii gene for 16S rRNA, partial sequence	AB041226	1398	GenBank ENA	383372 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Roseiflexus castenholzii DSM 13941	GCA_000017805	complete	GenBank ENA	383372 tax ID	*
[Ref.: #66792]	Roseiflexus castenholzii DSM 13941	383372.6	complete	PATRIC	383372 tax ID	*
[Ref.: #66792]	Roseiflexus castenholzii HLO8, DSM 13941	640753047	complete	JGI IMG/M	383372 tax ID	*

[Ref.: #5192]	GC-content	62.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #23075]	GC-content	62.0 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	60.7 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	62 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Roseiflexus castenholzii DSM 13941 DSM 13941 NCBI: GCA_000017805.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	69	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Roseiflexus castenholzii DSM 13941 DSM 13941 NCBI: GCA_000017805.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	52.243	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	55.686	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	56.582	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	54.472	no
[Ref.: #69480]	thermophilic	thermophileⓘ	yes	54.679	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	77.524	no

Availability in culture collections External links

[Ref.: #5192]

Culture collection no.

DSM 13941, JCM 11240, NBRC 100045

[Ref.: #71966]

SI-ID 49536

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes.	Hanada S, Takaichi S, Matsuura K, Nakamura K	Int J Syst Evol Microbiol	10.1099/00207713-52-1-187	2002	*

References

#5192

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13941

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23075

Satoshi Hanada,Shinichi Takaichi,Katsumi Matsuura,Kazunori Nakamura: Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes.. IJSEM 52: 187 - 193 2002 ( DOI 10.1099/00207713-52-1-187 , PubMed 11837302 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71966

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49536.1 )

* These data were automatically processed and therefore are not curated

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Roseiflexus castenholzii DSM 13941 DSM 13941

Roseiflexus castenholzii DSM 13941 DSM 13941

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