Haloferax volcanii (DSM 27206, CIP 107620, JCM 10717)

Name: Haloferax volcanii (DSM 27206, CIP 107620, JCM 10717)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24079

Type strain

Strain Designation TM

Culture col. no. DSM 27206 CIP 107620 JCM 10717 KCTC 12962 NBRC 16590 3 more

NCBI tax ID(s) 2246

Special Collections DSMZ JCM KCTC CIP

Links

Haloferax volcanii TM is a mesophilic, Gram-negative, rod-shaped prokaryote that was isolated from solar saltern.

Gram-negative rod-shaped mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 100673

IFO 16590,JCM 10717,KCTC 12962,CIP 107620,NBRC 16590,DSM 27206

@ref 20215

Last LPSN update 2025-12-16 09:52:46

Domain Methanobacteriati

Phylum Methanobacteriota

Class Halobacteria

Order Halobacteriales

Family Haloferacaceae

Genus Haloferax

Species Haloferax volcanii

Full scientific name Haloferax volcanii (Mullakhanbhai and Larsen 1975) Torreblanca et al. 1986

Synonyms (6)

Haloferax alexandrinum
Halobacterium volcanii
Haloferax lucentensis
"Haloferax alicantei"
Haloferax alexandrinus
Haloferax lucentense

BacDive ID	Other strains from **Haloferax volcanii** (2)	Type strain
5933	H. volcanii DS 2, DS2, DSM 3757, ATCC 29605, CCM 2852, ... (type strain)
5934	H. volcanii WFD II, DSM 5716

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
116217	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
20968	HALOBACTERIA MEDIUM (DSMZ Medium 372)	Medium recipe at MediaDive	Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water
39909	MEDIUM 204 - for Halorubrum, Marinococcus and Natrialba		Distilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (2.000 g);ManganeseII chloride tetrahydrate (0.360 mg);Magnesium sulphate heptahydrate (20.000 g);Agar (20.000 g);Yeast extract (5.000 g);Ferrous chloride tetrahydrate
116217	CIP Medium 330	Medium recipe at CIP
116217	CIP Medium 204	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
20968	positive	growth	37	mesophilic
39909	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
116217	positive	growth	25-45
116217	negative	growth	5
116217	negative	growth	15

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		96.4

Halophily

@ref	Salt	Tested relation	Concentration
116217	NaCl	growth	0 %
116217	NaCl	growth	2 %
116217	NaCl	growth	4 %
116217	NaCl	growth	6 %
116217	NaCl	growth	8 %
116217	NaCl	growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
116217	16947 ChEBI	citrate	-	carbon source
116217	4853 ChEBI	esculin	-	hydrolysis
116217	606565 ChEBI	hippurate	-	hydrolysis
116217	15792 ChEBI	malonate	-	assimilation
116217	17632 ChEBI	nitrate	-	builds gas from
116217	17632 ChEBI	nitrate	-	reduction
116217	16301 ChEBI	nitrite	-	builds gas from
116217	16301 ChEBI	nitrite	-	reduction

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
116217	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116217	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
116217	15688 ChEBI	acetoin	-
116217	17234 ChEBI	glucose		+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
116217	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
116217	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
116217	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116217	caseinase	-	3.4.21.50
116217	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
116217	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
116217	gamma-glutamyltransferase	-	2.3.2.2
116217	gelatinase	-
116217	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
116217	lipase	-
68382	lipase (C 14)	-		from API zym
116217	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
116217	ornithine decarboxylase	-	4.1.1.17
116217	oxidase	+
116217	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
116217	tryptophan deaminase	-
116217	tween esterase	-
116217	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116217	-	-	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
116217	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Condition	#Saline

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
20968	solar saltern	Alexandria	Egypt	EGY	Africa
67770	Solar saltern	Alexandria	Egypt	EGY	Africa
116217	Solar saltern	Alexandria	Egypt	EGY	Africa	1996

Taxonmaps

69479

Global distribution of 16S sequence AB037474 (>99% sequence identity) for Haloferax from Microbeatlas

Aquatic Samples	7
Soil Samples	1
Animal Samples
Plant Samples
Total Samples	8

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
20968	1	Risk group (German classification)
116217	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM33673v1 assembly for Haloferax volcanii JCM 10717	contig	GCA_000336735	1227458.3	2554235481	1227458	71.15

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20968	Haloferax alexandrinus gene for 16S ribosomal RNA, complete sequence	AB037474	1472	114529
124043	Haloferax alexandrinus gene for 16S rRNA, complete sequence, strain: JCM 10717.	AB663373	1473	114529
124043	Haloferax alexandrinus partial 16S rRNA gene, strain KCTC 12962	FR669667	1436	114529

GC content

@ref	GC-content (mol%)	Method
20968	59.5	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Haloferax alexandrinus JCM 10717
NCBI: GCA_000336735

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.40	no
125439	motility	BacteriaNetⓘ	yes	69.60	no
125439	gram_stain	BacteriaNetⓘ	negative	82.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	58.70	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Nitrate reduction in Haloferax alexandrinus: the case of assimilatory nitrate reductase.	Kilic V, Kilic GA, Kutlu HM, Martinez-Espinosa RM	Extremophiles	10.1007/s00792-017-0924-4	2017
Phylogeny	Haloferax alexandrinus sp. nov., an extremely halophilic canthaxanthin-producing archaeon from a solar saltern in Alexandria (Egypt).	Asker D, Ohta Y	Int J Syst Evol Microbiol	10.1099/00207713-52-3-729	2002

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20968	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27206
#39909	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116217	Collection of Institut Pasteur ; Curators of the CIP; CIP 107620
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Haloferax volcanii (DSM 27206, CIP 107620, JCM 10717)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000336735

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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