Name: Blastococcus endophyticus DSM 45413
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24054

Type strain:

Species: Blastococcus endophyticus

Culture col. no.: DSM 45413, CCTCC AA 209045, KCTC 19998, YIM 68236, JCM 17896, JCM 31483

Strain history: <- S-K Tang, Yunnan Inst., China

NCBI tax ID(s): 673521 (species)

Section

Blastococcus endophyticus DSM 45413 is an aerobe, mesophilic, Gram-positive bacterium that has a white to pink to black pigmentation and was isolated from surface sterilized, healthy leaves of the traditional Chinese medicinal plant Camptotheca acuminata.

Gram-positive
pigmented
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Geodermatophilales*
	Family	*Geodermatophilaceae*
	Genus	*Blastococcus*
	Species	**Blastococcus endophyticus**
	Full Scientific Name (PNU)	Blastococcus endophyticus Zhu et al. 2013

Synonym

"Trujillonella endophytica"

Information on morphological and physiological properties Morphology

[Ref.: #30996]	Gram stain	positive
[Ref.: #30996]	Motility	no

[Ref.: #67771]	Gram stain	positive

[Ref.: #24981]

Colony color

white to pink to black

[Ref.: #24981]	Pigment production	yes
[Ref.: #24981]	Pigmentation color	white to pink to black

Multimedia content

[Ref.: #20463]

Caption:

Medium 535 28°C

[Ref.: #20463]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20463]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #20463]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

[Ref.: #20463]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #20463]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

[Ref.: #20463]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #20463]

Culture medium growth

[Ref.: #20463]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #20463]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #20463]	growth	28 °C
[Ref.: #30996]	growth	10-45 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #20463]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH	pH range
[Ref.: #30996]			optimum	7
[Ref.: #30996]			growth	5.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #30996]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30996]		NaCl		growth	0-3	%

[Ref.: #67770]	Observation	quinones: MK-9(H₄)
[Ref.: #67771]	Observation	quinones: MK-9(H4), MK-9(H6) (4.2%), MK-8 (4.6%)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24981]	2-oxobutanoate ChEBI	-	oxidation
[Ref.: #24981]	3-hydroxybutyrate ChEBI	+/-	oxidation
[Ref.: #30996]	arabinose ChEBI	+	carbon source
[Ref.: #24981]	butyrate ChEBI	-	oxidation
[Ref.: #24981]	D-aspartate ChEBI	+/-	oxidation
[Ref.: #24981]	D-galacturonic acid ChEBI	+/-	oxidation
[Ref.: #24981]	D-glucarate ChEBI	+	oxidation
[Ref.: #24981]	D-gluconate ChEBI	+	oxidation
[Ref.: #24981]	D-mannitol ChEBI	+/-	oxidation
[Ref.: #24981]	D-sorbitol ChEBI	+	oxidation
[Ref.: #24981]	dextrin ChEBI	+	oxidation
[Ref.: #30996]	esculin ChEBI	+	hydrolysis
[Ref.: #24981]	galactarate ChEBI	-	oxidation
[Ref.: #24981]	gelatin ChEBI	+/-	oxidation
[Ref.: #24981]	gentiobiose ChEBI	+	oxidation
[Ref.: #24981]	L-alanine ChEBI	+/-	oxidation
[Ref.: #24981]	L-arginine ChEBI	-	oxidation
[Ref.: #24981]	L-aspartate ChEBI	-	oxidation
[Ref.: #24981]	methyl D-glucoside ChEBI	+	oxidation
[Ref.: #24981]	N-acetylglucosamine ChEBI	+/-	oxidation
[Ref.: #24981]	n-acetylneuraminate ChEBI	-	oxidation
[Ref.: #24981]	pectin ChEBI	-	oxidation
[Ref.: #30996]	ribose ChEBI	+	carbon source
[Ref.: #24981]	salicin ChEBI	+/-	oxidation
[Ref.: #24981]	sodium bromate ChEBI	+	oxidation
[Ref.: #30996]	tween 40 ChEBI	+	carbon source
[Ref.: #30996]	tween 80 ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30996]	acid phosphatase	+	3.1.3.2
[Ref.: #30996]	alkaline phosphatase	+	3.1.3.1
[Ref.: #24981]	catalase	+	1.11.1.6
[Ref.: #30996]	catalase	+	1.11.1.6
[Ref.: #24981]	cytochrome oxidase	-	1.9.3.1

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	glycine betaine biosynthesis	100	5 of 5
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	molybdenum cofactor biosynthesis	100	9 of 9
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	glycogen metabolism	100	5 of 5
[Ref.: #66794]	teichoic acid biosynthesis	100	1 of 1
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	100	6 of 6
[Ref.: #66794]	starch degradation	100	10 of 10
[Ref.: #66794]	ethylmalonyl-CoA pathway	100	5 of 5
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	aerobactin biosynthesis	100	1 of 1
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	valine metabolism	100	9 of 9
[Ref.: #66794]	ethanol fermentation	100	2 of 2
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	threonine metabolism	100	10 of 10
[Ref.: #66794]	taurine degradation	100	1 of 1
[Ref.: #66794]	leucine metabolism	92.31	12 of 13
[Ref.: #66794]	pentose phosphate pathway	90.91	10 of 11
[Ref.: #66794]	Entner Doudoroff pathway	90	9 of 10
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	flavin biosynthesis	86.67	13 of 15
[Ref.: #66794]	heme metabolism	85.71	12 of 14
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	glutathione metabolism	85.71	12 of 14
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	vitamin B1 metabolism	84.62	11 of 13
[Ref.: #66794]	vitamin B12 metabolism	82.35	28 of 34
[Ref.: #66794]	pyrimidine metabolism	82.22	37 of 45
[Ref.: #66794]	purine metabolism	81.91	77 of 94
[Ref.: #66794]	phenylacetate degradation (aerobic)	80	4 of 5
[Ref.: #66794]	factor 420 biosynthesis	80	4 of 5
[Ref.: #66794]	3-chlorocatechol degradation	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	citric acid cycle	78.57	11 of 14
[Ref.: #66794]	4-hydroxymandelate degradation	77.78	7 of 9
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	allantoin degradation	77.78	7 of 9
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	lipid metabolism	77.42	24 of 31
[Ref.: #66794]	urea cycle	76.92	10 of 13
[Ref.: #66794]	methionine metabolism	76.92	20 of 26
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	dTDPLrhamnose biosynthesis	75	6 of 8
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	glutamate and glutamine metabolism	75	21 of 28
[Ref.: #66794]	toluene degradation	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	gluconeogenesis	75	6 of 8
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	cyclohexanol degradation	75	3 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	75	9 of 12
[Ref.: #66794]	non-pathway related	73.68	28 of 38
[Ref.: #66794]	tryptophan metabolism	73.68	28 of 38
[Ref.: #66794]	3-phenylpropionate degradation	73.33	11 of 15
[Ref.: #66794]	tyrosine metabolism	71.43	10 of 14
[Ref.: #66794]	ubiquinone biosynthesis	71.43	5 of 7
[Ref.: #66794]	tetrahydrofolate metabolism	71.43	10 of 14
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	70.59	12 of 17
[Ref.: #66794]	4-hydroxyphenylacetate degradation	70	7 of 10
[Ref.: #66794]	propionate fermentation	70	7 of 10
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	daunorubicin biosynthesis	66.67	6 of 9
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	isoprenoid biosynthesis	65.38	17 of 26
[Ref.: #66794]	phenol degradation	65	13 of 20
[Ref.: #66794]	proline metabolism	63.64	7 of 11
[Ref.: #66794]	metabolism of disaccharids	63.64	7 of 11
[Ref.: #66794]	oxidative phosphorylation	62.64	57 of 91
[Ref.: #66794]	arginine metabolism	62.5	15 of 24
[Ref.: #66794]	androgen and estrogen metabolism	62.5	10 of 16
[Ref.: #66794]	cysteine metabolism	61.11	11 of 18
[Ref.: #66794]	creatinine degradation	60	3 of 5
[Ref.: #66794]	arachidonate biosynthesis	60	3 of 5
[Ref.: #66794]	gallate degradation	60	3 of 5
[Ref.: #66794]	cellulose degradation	60	3 of 5
[Ref.: #66794]	carotenoid biosynthesis	59.09	13 of 22
[Ref.: #66794]	histidine metabolism	58.62	17 of 29
[Ref.: #66794]	alanine metabolism	58.62	17 of 29
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	degradation of sugar alcohols	56.25	9 of 16
[Ref.: #66794]	d-xylose degradation	54.55	6 of 11
[Ref.: #66794]	sulfate reduction	53.85	7 of 13
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	53.85	7 of 13
[Ref.: #66794]	degradation of sugar acids	52	13 of 25
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	resorcinol degradation	50	1 of 2
[Ref.: #66794]	sphingosine metabolism	50	3 of 6
[Ref.: #66794]	mannosylglycerate biosynthesis	50	1 of 2
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	degradation of hexoses	50	9 of 18
[Ref.: #66794]	catecholamine biosynthesis	50	2 of 4
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	lysine metabolism	47.62	20 of 42
[Ref.: #66794]	degradation of pentoses	46.43	13 of 28
[Ref.: #66794]	phenylpropanoid biosynthesis	46.15	6 of 13
[Ref.: #66794]	vitamin B6 metabolism	45.45	5 of 11
[Ref.: #66794]	lipid A biosynthesis	44.44	4 of 9
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	benzoyl-CoA degradation	42.86	3 of 7
[Ref.: #66794]	ascorbate metabolism	40.91	9 of 22
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	polyamine pathway	39.13	9 of 23
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	chlorophyll metabolism	33.33	6 of 18
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	mevalonate metabolism	28.57	2 of 7
[Ref.: #66794]	aclacinomycin biosynthesis	28.57	2 of 7
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	alginate biosynthesis	25	1 of 4
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20463]	Sample type/isolated from	surface sterilized, healthy leaves of the traditional Chinese medicinal plant Camptotheca acuminata
[Ref.: #20463]	Host species	Camptotheca acuminata
[Ref.: #20463]	Geographic location (country and/or sea, region)	Yunnan Province
[Ref.: #20463]	Country	China
[Ref.: #20463]	Country ISO 3 Code	CHN
[Ref.: #20463]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Leaves of Camptotheca acuminata in Yunnan Province
[Ref.: #67770]	Host species	Camptotheca acuminata
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From leaves of' Camptotheca acuminata' Decne.
[Ref.: #67771]	Geographic location (country and/or sea, region)	Yunnan Province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Leaf (Phyllosphere)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20463]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20463]	Blastococcus endophyticus strain YIM 68236 16S ribosomal RNA gene, partial sequence	GQ494034	1435	ENA	673521 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Blastococcus endophyticus strain DSM 45413	673521.3	wgs	PATRIC	673521 tax ID	*
[Ref.: #66792]	Blastococcus endophyticus DSM 45413	2599185194	draft	JGI IMG/M	673521 tax ID	*
[Ref.: #67770]	Blastococcus endophyticus DSM 45413	GCA_900110555	scaffold	GenBank	673521 tax ID
[Ref.: #66792]	Succinivibrionaceae bacterium HRGM_Genome_2896	GCA_019421435	scaffold	PATRIC	2053620 tax ID	*

[Ref.: #20463]	GC-content	71.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30996]	GC-content	71.6 mol%

Availability in culture collections External links

[Ref.: #20463]

Culture collection no.

DSM 45413, CCTCC AA 209045, KCTC 19998, YIM 68236, JCM 17896, JCM 31483

[Ref.: #20218]

Associated Passport(s) in StrainInfo

891006, 891005, 891007, 891004

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Blastococcus endophyticus sp. nov., an actinobacterium isolated from Camptotheca acuminata.	Zhu WY, Zhang JL, Qin YL, Xiong ZJ, Zhang DF, Klenk HP, Zhao LX, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.049239-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#20463

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45413

#24981

K. L. Hezbri, Moussa,Nouioui, Imen,Gtari, Maher,Rohde, Manfred,Spröer, Cathrin,Schumann, Peter,Klenk, Hans-Peter,Ghodhbane-Gtari, Faten,Montero-Calasanz, Maria del Carmen: Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus. IJSEM 66: 4864 - 4872 2016 ( DOI 10.1099/ijsem.0.001443 , PubMed 27553620 )

#30996

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27326 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#27326

IJSEM 3269 2013 ( DOI 10.1099/ijs.0.049239-0 , PubMed 23475341 )

* These data were automatically processed and therefore are not curated

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