Name: Flavobacterium cutihirudinis E89
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24023

Type strain:

Species: Flavobacterium cutihirudinis

Strain Designation: E89

Culture col. no.: DSM 25795, CIP 110384, LMG 26922

Strain history: CIP <- 2012, P. Kämpfer, Giessen Univ., Giessen, Germany: strain E89

NCBI tax ID(s): 1265740 (species)

SI-ID 379501

LMG 26922, CIP 110374, DSM 25795, CIP 110384

Other strains from Flavobacterium cutihirudinis

F. cutihirudinis SF001207(FSU),

Section

Flavobacterium cutihirudinis E89 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from skin of a juvenile medical leech from a leech farm.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Flavobacterium*
	Species	**Flavobacterium cutihirudinis**
	Full Scientific Name (LPSN)	Flavobacterium cutihirudinis Glaeser et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30971]	Gram stain	negative
[Ref.: #118826]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.987

[Ref.: #30971]	Cell length	2.5 µm

[Ref.: #30971]	Cell width	0.9 µm

[Ref.: #30971]	Cell shape	rod-shaped
[Ref.: #118826]	Cell shape	rod-shaped

[Ref.: #30971]	Motility	yes
[Ref.: #118826]	Motility	yes

[Ref.: #30971]	Flagellum arrangement	gliding

[Ref.: #30971]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #37940]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

[Ref.: #37940]

Culture medium growth

[Ref.: #37940]

Culture medium composition

expand / minimize

[Ref.: #20570]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #20570]

Culture medium growth

[Ref.: #20570]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20570]

Culture medium composition

expand / minimize

[Ref.: #118826]

Culture medium

CIP Medium 566

[Ref.: #118826]

Culture medium growth

[Ref.: #118826]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #20570]	growth	28 °C
[Ref.: #30971]	growth	10-30 °C
[Ref.: #30971]	optimum	26.5 °C
[Ref.: #37940]	growth	25 °C

[Ref.: #20570]	Temperature range	mesophilic
[Ref.: #30971]	Temperature range	mesophilic
[Ref.: #37940]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30971]			optimum	8
[Ref.: #30971]			growth	6.5-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30971]	Oxygen tolerance	aerobe
[Ref.: #118826]	Oxygen tolerance	obligate aerobe

[Ref.: #30971]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30971]		NaCl		growth	0-3	%

[Ref.: #30971]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30971]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	galactose ChEBI	+	carbon source
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #30971]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #118826]	nitrate ChEBI	+	reduction
[Ref.: #118826]	nitrite ChEBI	+	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	pyruvate ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	sorbitol ChEBI	+	carbon source
[Ref.: #30971]	sucrose ChEBI	+	carbon source
[Ref.: #30971]	trehalose ChEBI	+	carbon source
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #30971]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #118826]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118826]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #30971]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #30971]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118826]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118826]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #118826]	11766	not determinedn.d.	-	-	-	+	-	+/-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	+	-	+	-	+	+	-	-	+/-	-	+/-	-	+/-	+/-	+/-	-	+/-	-	-	-	-	+/-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118826]	12563	-	+	-	-	-	+	+	-	-	-	+	+	-	-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20570]	Sample type/isolated from	skin of a juvenile medical leech (Hirudo verbana) from a leech farm
[Ref.: #20570]	Host species	Hirudo verbana
[Ref.: #20570]	Geographic location (country and/or sea, region)	Hessen, Biebertal
[Ref.: #20570]	Country	Germany
[Ref.: #20570]	Country ISO 3 Code	DEU
[Ref.: #20570]	Continent	Europe

[Ref.: #118826]	Sample type/isolated from	Animal, Surface of a juvenile leech, Hirudo verbana
[Ref.: #118826]	Geographic location (country and/or sea, region)	Leech farm , Biebertal, Hessen
[Ref.: #118826]	Country	Germany
[Ref.: #118826]	Country ISO 3 Code	DEU
[Ref.: #118826]	Continent	Europe
[Ref.: #118826]	Isolation date	2009
* marker position based on {}

Isolation sources categories

#Host	#Invertebrates (Other)	#Annelida
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20570]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118826]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20570]	Flavobacterium cutihirudinis strain E89 16S ribosomal RNA gene, partial sequence	JX966231	1373	ENA	1265740 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Flavobacterium cutihirudinis DSM 25795	GCA_003385895	scaffold	GenBank	1265740 tax ID	*
[Ref.: #66792]	Flavobacterium cutihirudinis strain DSM 25795	1265740.4	wgs	PATRIC	1265740 tax ID	*
[Ref.: #66792]	Flavobacterium cutihirudinis DSM 25795	2695421040	draft	JGI IMG/M	1265740 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Flavobacterium cutihirudinis DSM 25795 NCBI: GCA_003385895.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Flavobacterium cutihirudinis DSM 25795 NCBI: GCA_003385895.1
[Ref.: #69480]	flagellated	no	93.016	yes
[Ref.: #69480]	gram-positive	no	98.2	yes
[Ref.: #69480]	anaerobic	no	99.685	no
[Ref.: #69480]	aerobic	yes	88.792	no
[Ref.: #69480]	halophile	no	96.45	no
[Ref.: #69480]	spore-forming	no	94.544	yes
[Ref.: #69480]	glucose-util	yes	93.233	yes
[Ref.: #69480]	thermophile	no	99.602	yes
[Ref.: #69480]	motile	no	77.983	no
[Ref.: #69480]	glucose-ferment	no	87.964	no

Availability in culture collections External links

[Ref.: #20570]

Culture collection no.

DSM 25795, CIP 110384, LMG 26922

[Ref.: #87997]

SI-ID 379501

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flavobacterium cutihirudinis sp. nov., isolated from the skin of the medical leech Hirudo verbana.	Glaeser SP, Galatis H, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.048736-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20570

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25795

#30971

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27301 (see below)

#37940 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87997

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID379501.1 )

#118826 Collection of Institut Pasteur ; Curators of the CIP; CIP 110384

#27301

IJSEM 2841 2013 ( DOI 10.1099/ijs.0.048736-0 , PubMed 23315416 )

* These data were automatically processed and therefore are not curated

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Flavobacterium cutihirudinis DSM 25795

Flavobacterium cutihirudinis DSM 25795

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