Name: Enterococcus diestrammenae ORL-24
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24004

Type strain:

Species: Enterococcus diestrammenae

Strain Designation: ORL-24

Culture col. no.: DSM 29072, JCM 18359, KACC 16708

Strain history: J.-W. Bae ORL-24.

NCBI tax ID(s): 1155073 (species)

SI-ID 402198

DSM 29072

Section

Enterococcus diestrammenae ORL-24 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from gut of the camel cricket Diestrammena coreana.

coccus-shaped
Gram-positive
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus diestrammenae**
	Full Scientific Name (LPSN)	Enterococcus diestrammenae Kim et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #31026]	Gram stain	positive

[Ref.: #31026]	Cell length	1.15 µm

[Ref.: #31026]	Cell width	1.15 µm

[Ref.: #31026]	Cell shape	coccus-shaped

[Ref.: #31026]	Motility	no

[Ref.: #20958]	Type of hemolysis	alpha/beta
[Ref.: #20958]	Hemolysis ability	1
[Ref.: #20958]	Incubation period	1-2 days

[Ref.: #31026]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20958]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #20958]

Culture medium growth

[Ref.: #20958]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20958]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #20958]	growth	37 °C
[Ref.: #31026]	growth	10-37 °C
[Ref.: #31026]	optimum	33.5 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #20958]	Temperature range	mesophilic
[Ref.: #31026]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31026]			optimum	8
[Ref.: #31026]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #20958]	Oxygen tolerance	anaerobe
[Ref.: #31026]	Oxygen tolerance	facultative anaerobe

[Ref.: #31026]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31026]		NaCl		growth	0-5	%

[Ref.: #31026]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31026]	2-oxopentanoate ChEBI	+	carbon source
[Ref.: #68381]	alpha-cyclodextrin ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	L-arabinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #31026]	lactate ChEBI	+	carbon source
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #31026]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #31026]	pyruvate ChEBI	+	carbon source
[Ref.: #68381]	raffinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #31026]	thymidine ChEBI	+	carbon source
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #31026]	uridine ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	no	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	-	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #31026]	acid phosphatase	+	3.1.3.2
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	-	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	-	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #20958]	catalase	-	1.11.1.6
[Ref.: #20958]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #20958]	10625	-	+	+	-	-	-	+/-	-	-	+/-	+	-	-	+	-	+	-	-	+	-	+	+	-	-	-	+	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20958]	Sample type/isolated from	gut of the camel cricket Diestrammena coreana
[Ref.: #20958]	Host species	Diestrammena coreana
[Ref.: #20958]	Country	Republic of Korea
[Ref.: #20958]	Country ISO 3 Code	KOR
[Ref.: #20958]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Gut of the camel cricket
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20958]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Enterococcus diestrammenae JCM 18359 gene for 16S rRNA, partial sequence	LC521987	1489	ENA	1155073 tax ID
[Ref.: #20958]	Enterococcus diestrammenae strain ORL-24 16S ribosomal RNA gene, partial sequence	JQ650245	1439	ENA	1155073 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus diestrammenae strain DSM 29072	1155073.5	wgs	PATRIC	1155073 tax ID	*
[Ref.: #66792]	Enterococcus diestrammenae DSM 29072	2901213754	draft	JGI IMG/M	1155073 tax ID	*

[Ref.: #31026]	GC-content	41.3 mol%
[Ref.: #67770]	GC-content	41.3 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus diestrammenae strain DSM 29072 PATRIC: 1155073.5
[Ref.: #69480]	motile	no	93.86	yes
[Ref.: #69480]	gram-positive	yes	93.596	no
[Ref.: #69480]	anaerobic	no	95.407	no
[Ref.: #69480]	aerobic	no	96.609	yes
[Ref.: #69480]	halophile	yes	73.43	no
[Ref.: #69480]	spore-forming	no	88.308	yes
[Ref.: #69480]	flagellated	no	97.397	no
[Ref.: #69480]	thermophile	no	98.794	yes
[Ref.: #69480]	glucose-util	yes	89.431	no
[Ref.: #69480]	glucose-ferment	yes	85.04	no

Availability in culture collections External links

[Ref.: #20958]

Culture collection no.

DSM 29072, JCM 18359, KACC 16708

[Ref.: #87979]

SI-ID 402198

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Enterococcus diestrammenae sp. nov., isolated from the gut of Diestrammena coreana.	Kim JY, Shin NR, Na HK, Hyun DW, Whon TW, Kim PS, Yun JH, Bae JW	Int J Syst Evol Microbiol	10.1099/ijs.0.050062-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20958

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29072

#31026

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27356 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68381

Automatically annotated from API rID32STR .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#87979

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402198.1 )

#27356

IJSEM 4540 2013 ( DOI 10.1099/ijs.0.050062-0 , PubMed 23907226 )

* These data were automatically processed and therefore are not curated

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Enterococcus diestrammenae strain DSM 29072

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