Cronobacter universalis DSM 27963 is a Gram-negative, rod-shaped bacterium that was isolated from fresh water.
Gram-negative rod-shaped genome sequence 16S sequence Bacteria| @ref 20215 |
|
|
| Domain Bacteria |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Enterobacterales |
| Family Enterobacteriaceae |
| Genus Cronobacter |
| Species Cronobacter universalis |
| Full scientific name Cronobacter universalis Joseph et al. 2012 |
| 30278 | Productionyes |
| @ref: | 21154 |
| multimedia content: | DSM_27963.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_27963.jpg |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 21154 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68368 | 27613 ChEBI | amygdalin | + | fermentation | from API 20E |
| 68368 | 29016 ChEBI | arginine | + | hydrolysis | from API 20E |
| 30278 | 16947 ChEBI | citrate | + | carbon source | |
| 68368 | 16947 ChEBI | citrate | + | assimilation | from API 20E |
| 68368 | 17634 ChEBI | D-glucose | + | fermentation | from API 20E |
| 68368 | 5291 ChEBI | gelatin | + | hydrolysis | from API 20E |
| 68368 | 30849 ChEBI | L-arabinose | + | fermentation | from API 20E |
| 68368 | 62345 ChEBI | L-rhamnose | + | fermentation | from API 20E |
| 68368 | 25094 ChEBI | lysine | - | degradation | from API 20E |
| 30278 | 15792 ChEBI | malonate | + | carbon source | |
| 68368 | 28053 ChEBI | melibiose | + | fermentation | from API 20E |
| 68368 | 17268 ChEBI | myo-inositol | + | fermentation | from API 20E |
| 30278 | 17632 ChEBI | nitrate | + | reduction | |
| 68368 | 17632 ChEBI | nitrate | + | reduction | from API 20E |
| 30278 | 18257 ChEBI | ornithine | + | carbon source | |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 68368 | 30911 ChEBI | sorbitol | - | fermentation | from API 20E |
| 68368 | 27897 ChEBI | tryptophan | - | energy source | from API 20E |
| 68368 | 16199 ChEBI | urea | - | hydrolysis | from API 20E |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68368 | arginine dihydrolase | + | 3.5.3.6 | from API 20E |
| 68368 | beta-galactosidase | + | 3.2.1.23 | from API 20E |
| 30278 | catalase | + | 1.11.1.6 | |
| 68368 | cytochrome oxidase | - | 1.9.3.1 | from API 20E |
| 68368 | gelatinase | + | from API 20E | |
| 68368 | lysine decarboxylase | - | 4.1.1.18 | from API 20E |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 68368 | urease | - | 3.5.1.5 | from API 20E |
| @ref | ONPG | ADH (Arg) | LDC (Lys) | ODC | CIT | H2S productionH2S | URE | TDA (Trp) | IND | Acetoin production (Voges Proskauer test)VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX | Nitrite productionNO2 | Reduction to N2N2 | MotilityMOB | Growth on MacConkey mediumMAC | OF-O | OF-F | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 21154 | + | + | - | - | + | - | - | - | - | + | + | + | + | + | - | + | + | + | + | + | - | + | - | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | |
| 21154 | + | + | - | - | + | - | - | - | - | + | + | + | + | + | - | + | - | + | + | + | - | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | |
| 21154 | + | + | - | - | + | - | - | - | - | + | + | + | - | + | - | + | - | + | + | + | - | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. |
| @ref | Sample type | Country | Country ISO 3 Code | Continent | |
|---|---|---|---|---|---|
| 21154 | fresh water | United Kingdom | GBR | Europe |
Global distribution of 16S sequence EF059877 (>99% sequence identity) for Cronobacter from Microbeatlas ![]()
| @ref | Pathogenicity human | Biosafety level | Biosafety level comment | |
|---|---|---|---|---|
| 21154 | yes, in single cases | 1 | Risk group (German classification) |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM127717v1 assembly for Cronobacter universalis NCTC 9529 | complete | 1074000 | 98.12 | ||||
| 66792 | 37419_E02 assembly for Cronobacter universalis NCTC 9529 | contig | 1074000 | 77.91 | ||||
| 66792 | Cuni9529 final contigs assembly for Cronobacter universalis NCTC 9529 | contig | 1074000 | 71.02 | ||||
| 66792 | ASM31932v1 assembly for Cronobacter universalis NCTC 9529 | scaffold | 1074000 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | aerobe | 89.70 | no |
| 125439 | gram_stain | BacteriaNetⓘ | negative | 99.80 | no |
| 125439 | motility | BacteriaNetⓘ | yes | 74.50 | no |
| 125439 | spore_formation | BacteriaNetⓘ | no | 98.20 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | no | 99.50 | yes |
| 125438 | anaerobic | anaerobicⓘ | no | 96.30 | no |
| 125438 | aerobic | aerobicⓘ | no | 64.61 | no |
| 125438 | spore-forming | spore-formingⓘ | no | 89.30 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 100.00 | yes |
| 125438 | flagellated | motile2+ⓘ | yes | 77.17 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Comparative Analysis of Mesophilic YqfB-Type Amidohydrolases. | Statkeviciute R, Sadauskas M, Rainyte J, Kavaliauskaite K, Meskys R. | Biomolecules | 10.3390/biom12101492 | 2022 | ||
| Enzymology | Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii. | Aly MA, Domig KJ, Kneifel W, Reimhult E. | Sensors (Basel) | 10.3390/s18072028 | 2018 | |
| Detection of Cronobacter Genus in Powdered Infant Formula by Enzyme-linked Immunosorbent Assay Using Anti-Cronobacter Antibody. | Song X, Shukla S, Lee G, Park S, Kim M. | Front Microbiol | 10.3389/fmicb.2016.01124 | 2016 | ||
| Sampling informational properties of codon usage through the tree of life. | Martinez O, Reyes-Valdes MH, Ochoa-Alejo N. | PLoS One | 10.1371/journal.pone.0335824 | 2025 | ||
| Detection of Genus and Three Important Species of Cronobacter Using Novel Genus- and Species-Specific Genes Identified by Large-Scale Comparative Genomic Analysis. | Wang L, Wu P, Su Y, Wei Y, Guo X, Yang L, Wang M, Liu B. | Front Microbiol | 10.3389/fmicb.2022.885543 | 2022 | ||
| Genetics | Cronobacter spp. in Commercial Powdered Infant Formula Collected From Nine Provinces in China: Prevalence, Genotype, Biofilm Formation, and Antibiotic Susceptibility. | Fei P, Jing H, Ma Y, Dong G, Chang Y, Meng Z, Jiang S, Xie Q, Li S, Chen X, Yang W. | Front Microbiol | 10.3389/fmicb.2022.900690 | 2022 | |
| An Evaluation of the Sensitivity and Applicability of a Droplet Digital Polymerase Chain Reaction Assay to Simultaneously Detect Pseudomonas aeruginosa and Pseudomonas fragi in Foods. | Huang J, Zhai L, Wang J, Sun X, Wang B, Wei Z. | Foods | 10.3390/foods13101453 | 2024 | ||
| Biotechnology | Characterization of Cronobacter spp. isolated from food of plant origin and environmental samples collected from farms and from supermarkets in the Czech Republic. | Vojkovska H, Karpiskova R, Orieskova M, Drahovska H. | Int J Food Microbiol | 10.1016/j.ijfoodmicro.2015.10.017 | 2016 | |
| Multilocus sequence typing of Cronobacter spp. from powdered infant formula and milk powder production factories | Sonbol H, Joseph S, McAuley CM, Craven HM, Forsythe SJ. | Int Dairy J | 10.1016/j.idairyj.2012.11.004 | 2013 | ||
| Cronobacter spp.--opportunistic food-borne pathogens. A review of their virulence and environmental-adaptive traits. | Jaradat ZW, Al Mousa W, Elbetieha A, Al Nabulsi A, Tall BD. | J Med Microbiol | 10.1099/jmm.0.073742-0 | 2014 | ||
| Biotechnology | Comparison of methods for the microbiological identification and profiling of Cronobacter species from ingredients used in the preparation of infant formula. | Cetinkaya E, Joseph S, Ayhan K, Forsythe SJ. | Mol Cell Probes | 10.1016/j.mcp.2012.10.003 | 2013 | |
| Foodborne pathogens in Africa: Understanding Cronobacter sakazakii. | Mazi IM, Onyeaka H, Nnaji ND. | Public Health Chall | 10.1002/puh2.53 | 2023 | ||
| Genetics | Analysis of the Molecular Diversity Among Cronobacter Species Isolated From Filth Flies Using Targeted PCR, Pan Genomic DNA Microarray, and Whole Genome Sequencing Analyses. | Jang H, Chase HR, Gangiredla J, Grim CJ, Patel IR, Kothary MH, Jackson SA, Mammel MK, Carter L, Negrete F, Finkelstein S, Weinstein L, Yan Q, Iversen C, Pagotto F, Stephan R, Lehner A, Eshwar AK, Fanning S, Farber J, Gopinath GR, Tall BD, Pava-Ripoll M. | Front Microbiol | 10.3389/fmicb.2020.561204 | 2020 | |
| Quantitative Detection of Viable but Nonculturable Cronobacter sakazakii Using Photosensitive Nucleic Acid Dye PMA Combined with Isothermal Amplification LAMP in Raw Milk. | Hu L, Zhang S, Xue Y, Zhang Y, Zhang W, Wang S. | Foods | 10.3390/foods11172653 | 2022 | ||
| Prevalence, characterization, and antibiotic susceptibility of Cronobacter spp. in a milk powder processing environment: The first reported case in Serbia. | Csorba C, Pajic M, Blagojevic B, Forsythe S, Radinovic M, Velebit B. | Food Sci Nutr | 10.1002/fsn3.2681 | 2022 | ||
| Phylogeny | Genomic Analysis of Putative Virulence Factors Affecting Cytotoxicity of Cronobacter. | Cui J, Hu J, Du X, Yan C, Xue G, Li S, Cui Z, Huang H, Yuan J. | Front Microbiol | 10.3389/fmicb.2019.03104 | 2019 | |
| Genetics | Characterization of Cronobacter sakazakii Strains Originating from Plant-Origin Foods Using Comparative Genomic Analyses and Zebrafish Infectivity Studies. | Jang H, Eshwar A, Lehner A, Gangiredla J, Patel IR, Beaubrun JJ, Chase HR, Negrete F, Finkelstein S, Weinstein LM, Ko K, Addy N, Ewing L, Woo J, Lee Y, Seo K, Jaradat Z, Srikumar S, Fanning S, Stephan R, Tall BD, Gopinath GR. | Microorganisms | 10.3390/microorganisms10071396 | 2022 | |
| Comparative Proteomic Analysis of Adhesion/Invasion Related Proteins in Cronobacter sakazakii Based on Data-Independent Acquisition Coupled With LC-MS/MS. | Li P, Dong X, Wang XY, Du T, Du XJ, Wang S. | Front Microbiol | 10.3389/fmicb.2020.01239 | 2020 | ||
| Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Cronobacter sakazakii Isolates from Powdered Infant Formula Collected from Chinese Retail Markets. | Fei P, Jiang Y, Jiang Y, Yuan X, Yang T, Chen J, Wang Z, Kang H, Forsythe SJ. | Front Microbiol | 10.3389/fmicb.2017.02026 | 2017 | ||
| Genetics | Pan-genome analysis of the emerging foodborne pathogen Cronobacter spp. suggests a species-level bidirectional divergence driven by niche adaptation. | Grim CJ, Kotewicz ML, Power KA, Gopinath G, Franco AA, Jarvis KG, Yan QQ, Jackson SA, Sathyamoorthy V, Hu L, Pagotto F, Iversen C, Lehner A, Stephan R, Fanning S, Tall BD. | BMC Genomics | 10.1186/1471-2164-14-366 | 2013 | |
| Antibiotic and Desiccation Resistance of Cronobacter sakazakii and C. malonaticus Isolates from Powdered Infant Formula and Processing Environments. | Fei P, Jiang Y, Feng J, Forsythe SJ, Li R, Zhou Y, Man C. | Front Microbiol | 10.3389/fmicb.2017.00316 | 2017 | ||
| Biotechnology | Occurrence and Characterization of Cronobacter spp. in Dehydrated Rice Powder from Chinese Supermarket. | Huang Y, Pang Y, Wang H, Tang Z, Zhou Y, Zhang W, Li X, Tan D, Li J, Lin Y, Liu X, Huang W, Shi Y. | PLoS One | 10.1371/journal.pone.0131053 | 2015 | |
| Development of a Custom-Designed, Pan Genomic DNA Microarray to Characterize Strain-Level Diversity among Cronobacter spp. | Tall BD, Gangiredla J, Gopinath GR, Yan Q, Chase HR, Lee B, Hwang S, Trach L, Park E, Yoo Y, Chung T, Jackson SA, Patel IR, Sathyamoorthy V, Pava-Ripoll M, Kotewicz ML, Carter L, Iversen C, Pagotto F, Stephan R, Lehner A, Fanning S, Grim CJ. | Front Pediatr | 10.3389/fped.2015.00036 | 2015 | ||
| Phylogeny | Prevalence, Distribution, and Phylogeny of Type Two Toxin-Antitoxin Genes Possessed by Cronobacter Species where C. sakazakii Homologs Follow Sequence Type Lineages. | Finkelstein S, Negrete F, Jang H, Gangiredla J, Mammel M, Patel IR, Chase HR, Woo J, Lee Y, Wang CZ, Weinstein L, Tall BD, Gopinath GR. | Microorganisms | 10.3390/microorganisms7110554 | 2019 | |
| Proteome | Chemotaxis and Shorter O-Antigen Chain Length Contribute to the Strong Desiccation Tolerance of a Food-Isolated Cronobacter sakazakii Strain. | Qian C, Huang M, Du Y, Song J, Mu H, Wei Y, Zhang S, Yin Z, Yuan C, Liu B, Liu B. | Front Microbiol | 10.3389/fmicb.2021.779538 | 2021 | |
| Characterization of the Desiccation Tolerance of Cronobacter sakazakii Strains. | Du XJ, Wang XY, Dong X, Li P, Wang S. | Front Microbiol | 10.3389/fmicb.2018.02867 | 2018 | ||
| Genetics | Pan-genome diversification and recombination in Cronobacter sakazakii, an opportunistic pathogen in neonates, and insights to its xerotolerant lifestyle. | Lee IPA, Andam CP. | BMC Microbiol | 10.1186/s12866-019-1664-7 | 2019 | |
| Use of a Pan-Genomic DNA Microarray in Determination of the Phylogenetic Relatedness among Cronobacter spp. and Its Use as a Data Mining Tool to Understand Cronobacter Biology. | Tall BD, Gangiredla J, Grim CJ, Patel IR, Jackson SA, Mammel MK, Kothary MH, Sathyamoorthy V, Carter L, Fanning S, Iversen C, Pagotto F, Stephan R, Lehner A, Farber J, Yan QQ, Gopinath GR. | Microarrays (Basel) | 10.3390/microarrays6010006 | 2017 | ||
| Genetics | Reconstituting the History of Cronobacter Evolution Driven by Differentiated CRISPR Activity. | Zeng H, Zhang J, Wu Q, He W, Wu H, Ye Y, Li C, Ling N, Chen M, Wang J, Cai S, Lei T, Ding Y, Xue L. | Appl Environ Microbiol | 10.1128/aem.00267-18 | 2018 | |
| Comparative Outer Membrane Protein Analysis of High and Low-Invasive Strains of Cronobacter malonaticus. | Aldubyan MA, Almami IS, Benslimane FM, Alsonosi AM, Forsythe SJ. | Front Microbiol | 10.3389/fmicb.2017.02268 | 2017 | ||
| Natural Compounds With Antibacterial Activity Against Cronobacter spp. in Powdered Infant Formula: A Review. | Polat Yemis G, Delaquis P. | Front Nutr | 10.3389/fnut.2020.595964 | 2020 | ||
| Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance. | Feeney A, Johnston CD, Govender R, O'Mahony J, Coffey A, Sleator RD. | Gut Pathog | 10.1186/1757-4749-6-15 | 2014 | ||
| Screening of genes involved in interactions with intestinal epithelial cells in Cronobacter sakazakii. | Du XJ, Zhang X, Li P, Xue R, Wang S. | AMB Express | 10.1186/s13568-016-0246-4 | 2016 | ||
| The Secretion of Toxins and Other Exoproteins of Cronobacter: Role in Virulence, Adaption, and Persistence. | Jang H, Gopinath GR, Eshwar A, Srikumar S, Nguyen S, Gangiredla J, Patel IR, Finkelstein SB, Negrete F, Woo J, Lee Y, Fanning S, Stephan R, Tall BD, Lehner A. | Microorganisms | 10.3390/microorganisms8020229 | 2020 | ||
| Metabolism | Hfq plays important roles in virulence and stress adaptation in Cronobacter sakazakii ATCC 29544. | Kim S, Hwang H, Kim KP, Yoon H, Kang DH, Ryu S. | Infect Immun | 10.1128/iai.03161-14 | 2015 | |
| Analysis and Characterization of Proteins Associated with Outer Membrane Vesicles Secreted by Cronobacter spp. | Kothary MH, Gopinath GR, Gangiredla J, Rallabhandi PV, Harrison LM, Yan QQ, Chase HR, Lee B, Park E, Yoo Y, Chung T, Finkelstein SB, Negrete FJ, Patel IR, Carter L, Sathyamoorthy V, Fanning S, Tall BD. | Front Microbiol | 10.3389/fmicb.2017.00134 | 2017 | ||
| Cronobacter: an emergent pathogen causing meningitis to neonates through their feeds. | Tall BD, Chen Y, Yan Q, Gopinath GR, Grim CJ, Jarvis KG, Fanning S, Lampel KA. | Sci Prog | 10.3184/003685014x13994743930498 | 2014 | ||
| Genetics | Genomic characterization of malonate positive Cronobacter sakazakii serotype O:2, sequence type 64 strains, isolated from clinical, food, and environment samples. | Gopinath GR, Chase HR, Gangiredla J, Eshwar A, Jang H, Patel I, Negrete F, Finkelstein S, Park E, Chung T, Yoo Y, Woo J, Lee Y, Park J, Choi H, Jeong S, Jun S, Kim M, Lee C, Jeong H, Fanning S, Stephan R, Iversen C, Reich F, Klein G, Lehner A, Tall BD. | Gut Pathog | 10.1186/s13099-018-0238-9 | 2018 | |
| Genetics | Complete genome sequence and phenotype microarray analysis of Cronobacter sakazakii SP291: a persistent isolate cultured from a powdered infant formula production facility. | Yan Q, Power KA, Cooney S, Fox E, Gopinath GR, Grim CJ, Tall BD, McCusker MP, Fanning S. | Front Microbiol | 10.3389/fmicb.2013.00256 | 2013 | |
| Genetics | The structure of O-polysaccharide isolated from Cronobacter universalis NCTC 9529T. | Marszewska K, Czerwicka M, Forsythe SJ, Saldak E, Szulta S, Dziadziuszko H, Ossowska K, Kaczynski Z | Carbohydr Res | 10.1016/j.carres.2014.07.014 | 2014 | |
| Phylogeny | Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients. | Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.032292-0 | 2011 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #21154 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27963 |
| #26619 | IJSEM 1277 2012 ( DOI 10.1099/ijs.0.032292-0 , PubMed 22661070 ) |
| #30278 | Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26619 |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #68368 | Automatically annotated from API 20E . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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