Chlorobaculum tepidum (DSM 12025, ATCC 49652, TLS)

Name: Chlorobaculum tepidum (DSM 12025, ATCC 49652, TLS)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2395

Type strain

Strain Designation TLS

Culture col. no. DSM 12025 ATCC 49652 TLS

NCBI tax ID(s) 1097

Special Collections DSMZ Literature strains

History DSMZ <- M. T. Madigan

Links

Chlorobaculum tepidum TLS is an anaerobe, phototroph bacterium that was isolated from acidic high sulfide hot springs.

anaerobe phototroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 49224

DSM 12025,ATCC 49652,CIP 105973,NBRC 103806

@ref 20215

Last LPSN update 2026-05-29 08:25:27

Domain Bacteria

Phylum Chlorobiota

Class Chlorobiia

Order Chlorobiales

Family Chlorobiaceae

Genus Chlorobaculum

Species Chlorobaculum tepidum

Full scientific name Chlorobaculum tepidum (Wahlund et al. 1996) Imhoff 2003

Synonyms (1)

Chlorobium tepidum

Morphology

Cell morphology

@ref	Gram stain	Confidence	Cell width
23074			0.6-0.8 µm
125438	negative	92.026

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
4579	PFENNIG'S MEDIUM II (modified 1988, for green sulfur bacteria) (DSMZ Medium 29)		Medium recipe at MediaDive	Name: PFENNIG'S MEDIUM II (DSMZ Medium 29; with strain-specific modifications) Composition: NaHCO3 1.5 g/l Thiosulfate 1.0 g/l Na2S x 9 H2O 0.592592 g/l MgSO4 x 7 H2O 0.52 g/l Ammonium chloride 0.364 g/l KCl 0.364 g/l KH2PO4 0.364 g/l Dextrose 0.26 g/l Pyruvic acid sodium salt 0.26 g/l Ammonium acetate 0.26 g/l CaCl2 x 2 H2O 0.25 g/l Yeast extract 0.25 g/l Resazurin 0.00225 g/l HCl 0.002002 g/l FeSO4 x 7 H2O 0.00156 g/l Vitamin B12 0.001 g/l H3BO3 0.000312 g/l CoCl2 x 6 H2O 0.0001976 g/l MnCl2 x 4 H2O 0.000104 g/l ZnCl2 7.28e-05 g/l Na2MoO4 x 2 H2O 3.744e-05 g/l NiCl2 x 6 H2O 2.496e-05 g/l CuCl2 x 2 H2O 2.08e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
4579	positive	growth	47
23074

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
4579	anaerobe
23074	obligate anaerobe

Nutrition type

23074

Typephototroph

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23074	26710 ChEBI	sodium chloride	+	growth

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Acidic	-
#Condition	#Sulfuric	-
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4579	acidic high sulfide hot springs	Travelodge Stream, Sulphur Bay, Rotorna	New Zealand	NZL	Australia and Oceania

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4579	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM698v1 assembly for Chlorobaculum tepidum TLS	complete	GCA_000006985	194439.7	637000073	194439	92.91

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
23074	Chlorobaculum tepidum strain TLS 16S ribosomal RNA gene, partial sequence	M58468	1450		1097

GC content

@ref	GC-content (mol%)	Method
23074	56.5	Buoyant density centrifugation (BD)

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Chlorobaculum tepidum TLS TLS
NCBI: GCA_000006985.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	92.03	no
125438	anaerobic	anaerobicⓘ	yes	80.26	no
125438	aerobic	aerobicⓘ	no	82.98	yes
125438	spore-forming	spore-formingⓘ	no	83.68	no
125438	thermophilic	thermophileⓘ	no	57.78	yes
125438	flagellated	motile2+ⓘ	no	80.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Molecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria.	Puskar R, Du Truong C, Swain K, Chowdhury S, Chan KY, Li S, Cheng KW, Wang TY, Poh YP, Mazor Y, Liu H, Chou TF, Nannenga BL, Chiu PL.	Nat Commun	10.1038/s41467-022-33505-4	2022
Enzymology	Conversion of Escherichia coli into Mixotrophic CO₂ Assimilation with Malate and Hydrogen Based on Recombinant Expression of 2-Oxoglutarate:Ferredoxin Oxidoreductase Using Adaptive Laboratory Evolution.	Cheng YC, Huang WH, Lo SC, Huang E, Chiang EI, Huang CC, Yang YT.	Microorganisms	10.3390/microorganisms11020253	2023
Enzymology	hypD as a marker for [NiFe]-hydrogenases in microbial communities of surface waters.	Beimgraben C, Gutekunst K, Opitz F, Appel J.	Appl Environ Microbiol	10.1128/aem.00690-14	2014
	Pyrite stimulates the growth and sulfur oxidation capacity of anoxygenic phototrophic sulfur bacteria in euxinic environments.	Li R, Liu X, Wu G, Li G, Chen JH, Jiang H, Dong H.	Sci Adv	10.1126/sciadv.adu7080	2025
	Spectropolarimetry of Primitive Phototrophs as Global Surface Biosignatures.	Sparks WB, Parenteau MN, Blankenship RE, Germer TA, Patty CHL, Bott KM, Telesco CM, Meadows VS.	Astrobiology	10.1089/ast.2020.2272	2021
	Biosynthesis of unnatural glycolipids possessing diyne moiety in the acyl chain in the green sulfur photosynthetic bacterium Chlorobaculum tepidum grown by supplementation of 10,12-heptadecadiynic acid.	Saga Y, Yoshida N, Yamada S, Mizoguchi T, Tamiaki H.	Biochem Biophys Rep	10.1016/j.bbrep.2016.11.007	2017
Metabolism	Insights into the excitonic states of individual chlorosomes from Chlorobaculum tepidum.	Jendrny M, Aartsma TJ, Kohler J.	Biophys J	10.1016/j.bpj.2014.03.020	2014
	Large-Scale Molecular Evolutionary Analysis Uncovers a Variety of Polynucleotide Kinase Clp1 Family Proteins in the Three Domains of Life.	Saito M, Sato A, Nagata S, Tamaki S, Tomita M, Suzuki H, Kanai A.	Genome Biol Evol	10.1093/gbe/evz195	2019
Enzymology	Deciphering Evolutionary Trajectories of Lactate Dehydrogenases Provides New Insights into Allostery.	Robin AY, Brochier-Armanet C, Bertrand Q, Barette C, Girard E, Madern D.	Mol Biol Evol	10.1093/molbev/msad223	2023
Metabolism	Envelope proteins of the CsmB/CsmF and CsmC/CsmD motif families influence the size, shape, and composition of chlorosomes in Chlorobaculum tepidum.	Li H, Bryant DA.	J Bacteriol	10.1128/jb.00707-09	2009
Metabolism	Bacteriochlorophyllide c C-8(2) and C-12(1) methyltransferases are essential for adaptation to low light in Chlorobaculum tepidum.	Gomez Maqueo Chew A, Frigaard NU, Bryant DA.	J Bacteriol	10.1128/jb.00519-07	2007
Genetics	Genomic analysis reveals key aspects of prokaryotic symbiosis in the phototrophic consortium "Chlorochromatium aggregatum".	Liu Z, Muller J, Li T, Alvey RM, Vogl K, Frigaard NU, Rockwell NC, Boyd ES, Tomsho LP, Schuster SC, Henke P, Rohde M, Overmann J, Bryant DA.	Genome Biol	10.1186/gb-2013-14-11-r127	2013
Metabolism	Isorenieratene biosynthesis in green sulfur bacteria requires the cooperative actions of two carotenoid cyclases.	Maresca JA, Romberger SP, Bryant DA.	J Bacteriol	10.1128/jb.00758-08	2008
Phylogeny	Phylogeny of green sulfur bacteria on the basis of gene sequences of 16S rRNA and of the Fenna-Matthews-Olson protein.	Alexander B, Andersen JH, Cox RP, Imhoff JF	Arch Microbiol	10.1007/s00203-002-0432-4	2002

References

#4579	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12025
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23074	Johannes F. Imhoff: Phylogenetic taxonomy of the family Chlorobiaceae on the basis of 16S rRNA and fmo (Fenna-Matthews-Olson protein) gene sequences. IJSEM 53: 941 - 951 2003 ( DOI 10.1099/ijs.0.02403-0 , PubMed 12892110 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Chlorobaculum tepidum (DSM 12025, ATCC 49652, TLS)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Nutrition type

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000006985

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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