Name: Enterobacter sp. WS 20255
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23914

Type strain:

Species: Enterobacter sp.

Strain Designation: WS 20255

Culture col. no.: DSM 30060

Strain history: <- Bakteriologisches Institut der Sueddeutschen Versuchs- und Forschungsanstalt fuer Milchwirtschaft, Freising-Weihenstephan, Germany; WS 20255

NCBI tax ID(s): 2747372 (species)

SI-ID 113620

DSM 30060

Other strains from Enterobacter sp.

E. sp. WS 10183, DSM 30056
E. sp. CT 33, CT33, DSM 25355
E. sp. WS 20834, DSM 30111
E. sp. SCF1, DSM 25997
E. sp. EnspKS513, KS 513, DSM 29416
E. sp. T1-1, DSM 102032
E. sp. J49, DSM 105031
E. sp. CCUG 26141
E. sp. CCUG 39230
E. sp. CCUG 42838
E. sp. CCUG 45335
E. sp. CCUG 48344
E. sp. CCUG 48763
E. sp. CCUG 50044
E. sp. CCUG 52022
E. sp. CCUG 55030
E. sp. CCUG 58135, CB 163/09
E. sp. CCUG 69729
E. sp. CCUG 70166
E. sp. IMI 373955
E. sp. Stevenage 150518_C13, DSM 107791
E. sp. St_T4, DSM 110306
E. sp. JCM 17987
E. sp. JCM 17988
E. sp. JCM 17989
E. sp. JCM 17990

Section

Enterobacter sp. WS 20255 is a mesophilic, Gram-negative bacterium that was isolated from minced meat.

Gram-negative
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Enterobacter*
	Full Scientific Name (LPSN)	Enterobacter Hormaeche and Edwards 1960 (Approved Lists 1980)

Synonym

"Cloaca"

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.994

[Ref.: #69480]	Motility	yes Confidence in %97.458

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20543]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #20543]

Culture medium growth

[Ref.: #20543]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20543]

Culture medium composition

expand / minimize

[Ref.: #20543]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #20543]

Culture medium growth

[Ref.: #20543]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20543]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #20543]			growth	30 °C

[Ref.: #20543]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.955

[Ref.: #20543]

Name of produced compound

restriction endonuclease EccI

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20543]	17974	+	-	-	+	+	-	-	-	-	+	-	+	+	-	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20543]	Sample type/isolated from	minced meat
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Meat

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20543]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterobacter sp. DSM 30060	GCA_013375955	complete	GenBank	2747372 tax ID	*
[Ref.: #66792]	Enterobacter sp. DSM 30060	2747372.3	complete	PATRIC	2747372 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterobacter sp. DSM 30060 DSM 30060 NCBI: GCA_013375955.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterobacter sp. DSM 30060 DSM 30060 NCBI: GCA_013375955.1
[Ref.: #69480]	motile	yes	87.975	no
[Ref.: #69480]	flagellated	no	65.1	no
[Ref.: #69480]	gram-positive	no	97.972	no
[Ref.: #69480]	anaerobic	no	96.18	no
[Ref.: #69480]	aerobic	yes	83.982	no
[Ref.: #69480]	halophile	no	86.786	no
[Ref.: #69480]	spore-forming	no	93.024	no
[Ref.: #69480]	thermophile	no	99.148	no
[Ref.: #69480]	glucose-util	yes	95.024	no
[Ref.: #69480]	glucose-ferment	yes	93.359	yes

Availability in culture collections External links

[Ref.: #20543]

Culture collection no.

DSM 30060

[Ref.: #87921]

SI-ID 113620

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20543

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 30060

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87921

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID113620.1 )

* These data were automatically processed and therefore are not curated

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Enterobacter sp. DSM 30060 DSM 30060

Enterobacter sp. DSM 30060 DSM 30060

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