Name: Corynebacterium lactis RW2-5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23877

Type strain:

Species: Corynebacterium lactis

Strain Designation: RW2-5

Culture col. no.: DSM 45799, CCUG 63372, CIP 110786, JCM 32211

Strain history: CIP <- 2014, DSMZ <- A. Lipski, Bonn Univ., Bonn, Germany: strain RW2-5 <- R. Wierz, Bonn Univ., Bonn, Germany

NCBI tax ID(s): 1231000 (species)

SI-ID 404474

DSM 45799, CCUG 63372, CIP 110786

Section

Corynebacterium lactis RW2-5 is an aerobe, mesophilic, Gram-positive animal pathogen that was isolated from raw cow milk.

Gram-positive
rod-shaped
aerobe
animal pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium lactis**
	Full Scientific Name (LPSN)	Corynebacterium lactis Wiertz et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #31049]	Gram stain	positive
[Ref.: #120525]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31049]	Cell length	1.3 µm

[Ref.: #31049]	Cell width	0.7 µm

[Ref.: #31049]	Cell shape	rod-shaped
[Ref.: #120525]	Cell shape	rod-shaped

[Ref.: #31049]	Motility	no
[Ref.: #120525]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91.57

[Ref.: #31049]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #41609]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #41609]

Culture medium growth

[Ref.: #41609]

Culture medium composition

expand / minimize

[Ref.: #20517]

Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)

[Ref.: #20517]

Culture medium growth

[Ref.: #20517]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20517]

Culture medium composition

expand / minimize

[Ref.: #120525]

Culture medium

CIP Medium 72

[Ref.: #120525]

Culture medium growth

[Ref.: #120525]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #20517]	growth	28 °C
[Ref.: #31049]	growth	30-42 °C
[Ref.: #41609]	growth	30 °C
[Ref.: #62974]	growth	37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #20517]	Temperature range	mesophilic
[Ref.: #41609]	Temperature range	mesophilic
[Ref.: #62974]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31049]	Oxygen tolerance	aerobe
[Ref.: #62974]	Oxygen tolerance	microaerophile

[Ref.: #31049]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.983

[Ref.: #67770]

Observation

quinones: MK-9(H₂), MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31049]	fructose ChEBI	+	carbon source
[Ref.: #31049]	glucose ChEBI	+	carbon source
[Ref.: #31049]	mannose ChEBI	+	carbon source
[Ref.: #31049]	ribose ChEBI	+	carbon source

	Enzyme	Enzyme activity	EC number
[Ref.: #31049]	alkaline phosphatase	+	3.1.3.1
[Ref.: #31049]	alpha-galactosidase	+	3.2.1.22
[Ref.: #31049]	catalase	+	1.11.1.6
[Ref.: #31049]	pyrazinamidase	+	3.5.1.B15
[Ref.: #31049]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20517]	Sample type/isolated from	raw cow milk
[Ref.: #20517]	Geographic location (country and/or sea, region)	Bonn
[Ref.: #20517]	Country	Germany
[Ref.: #20517]	Country ISO 3 Code	DEU
[Ref.: #20517]	Continent	Europe

[Ref.: #62974]	Sample type/isolated from	Raw cow milk
[Ref.: #62974]	Sampling date	2010-10-19
[Ref.: #62974]	Country	Germany
[Ref.: #62974]	Country ISO 3 Code	DEU
[Ref.: #62974]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Raw cow's milk
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe

[Ref.: #120525]	Sample type/isolated from	Food, Raw cow milk
[Ref.: #120525]	Geographic location (country and/or sea, region)	Bonn
[Ref.: #120525]	Country	Germany
[Ref.: #120525]	Country ISO 3 Code	DEU
[Ref.: #120525]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HE983829 (>99% sequence identity) for Corynebacterium lactis subclade from Microbeatlas

Aquatic Samples	72
Soil Samples	114
Animal Samples	1114
Plant Samples	8
Total Samples	1308

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20517]	Pathogenicity (animal)	yes, in single cases
[Ref.: #20517]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120525]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20517]	Corynebacterium lactis partial 16S rRNA gene, type strain RW2-5T	HE983829	1452	ENA	1408189 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Corynebacterium lactis RW2-5	GCA_001274895	complete	GenBank	1408189 tax ID	*
[Ref.: #66792]	Corynebacterium lactis RW2-5	1408189.4	complete	PATRIC	1408189 tax ID	*
[Ref.: #66792]	Corynebacterium lactis RW2-5	2645727750	complete	JGI IMG/M	1408189 tax ID	*

[Ref.: #67770]

GC-content

60.5 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Corynebacterium lactis RW2-5 PATRIC: 1408189.4
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium lactis RW2-5 RW2-5 NCBI: GCA_001274895.1
[Ref.: #69480]	motile	no	93.185	yes
[Ref.: #69480]	flagellated	no	95.916	yes
[Ref.: #69480]	gram-positive	yes	87.228	no
[Ref.: #69480]	anaerobic	no	98.044	yes
[Ref.: #69480]	halophile	yes	61.113	no
[Ref.: #69480]	spore-forming	no	88.212	yes
[Ref.: #69480]	thermophile	no	93.083	yes
[Ref.: #69480]	glucose-util	yes	88.89	no
[Ref.: #69480]	aerobic	no	74.837	no
[Ref.: #69480]	glucose-ferment	no	51.178	no

Availability in culture collections External links

[Ref.: #20517]

Culture collection no.

DSM 45799, CCUG 63372, CIP 110786, JCM 32211

[Ref.: #87889]

SI-ID 404474

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Corynebacterium frankenforstense sp. nov. and Corynebacterium lactis sp. nov., isolated from raw cow milk.	Wiertz R, Schulz SC, Muller U, Kampfer P, Lipski A	Int J Syst Evol Microbiol	10.1099/ijs.0.050757-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20517

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45799

#31049

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27379 (see below)

#41609 ; Curators of the CIP;
#62974 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 63372

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87889

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404474.1 )

#120525 Collection of Institut Pasteur ; Curators of the CIP; CIP 110786

#27379

IJSEM 4495 2013 ( DOI 10.1099/ijs.0.050757-0 , PubMed 23907219 )

* These data were automatically processed and therefore are not curated

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Corynebacterium lactis RW2-5

Corynebacterium lactis RW2-5 RW2-5

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