Tepidibacillus fermentans (DSM 23802, VKM B-2671, STGH)

Name: Tepidibacillus fermentans (DSM 23802, VKM B-2671, STGH)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 23768

Type strain

Strain Designation STGH

Culture col. no. DSM 23802 VKM B-2671 STGH

NCBI tax ID(s) 1281767

Special Collections DSMZ

Links

Tepidibacillus fermentans STGH is an anaerobe, thermophilic prokaryote that was isolated from water from the observation well of an underground gas storage.

anaerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 398409

VKM B-2671,DSM 23802

@ref 20215

Last LPSN update 2025-12-16 09:52:43

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Tepidibacillus

Species Tepidibacillus fermentans

Full scientific name Tepidibacillus fermentans Slobodkina et al. 2014

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21270	VULCANIBACILLUS MEDIUM (DSMZ Medium 1042)		Medium recipe at MediaDive	Name: VULCANIBACILLUS MEDIUM (DSMZ Medium 1042; with strain-specific modifications) Composition: Sea Salt 29.8507 g/l D-Glucose 2.48756 g/l NaNO3 0.995025 g/l NaHCO3 0.995025 g/l Yeast extract 0.497512 g/l FeSO4 x 7 H2O 0.0995025 g/l MgSO4 x 7 H2O 0.0597015 g/l NaCl 0.0199005 g/l MnSO4 x H2O 0.00995025 g/l (NH4)2Ni(SO4)2 x 6 H2O 0.00557214 g/l CoCl2 x 6 H2O 0.00358209 g/l ZnSO4 x 7 H2O 0.00358209 g/l CaCl2 x 2 H2O 0.00199005 g/l Sodium resazurin 0.000497512 g/l AlK(SO4)2 x 12 H2O 0.000358209 g/l CuSO4 x 5 H2O 0.000199005 g/l H3BO3 0.000199005 g/l Na2MoO4 x 2 H2O 0.000199005 g/l Pyridoxine hydrochloride 0.000199005 g/l Na2SeO4 0.000199005 g/l Na2WO4 x 2 H2O 0.000199005 g/l Riboflavin 9.95025e-05 g/l Calcium D-(+)-pantothenate 9.95025e-05 g/l Nicotinic acid 9.95025e-05 g/l (DL)-alpha-Lipoic acid 9.95025e-05 g/l Thiamine HCl 9.95025e-05 g/l p-Aminobenzoic acid 9.95025e-05 g/l VOSO4 x 5 H2O 4.97512e-05 g/l Folic acid 3.9801e-05 g/l Biotin 3.9801e-05 g/l Vitamin B12 1.99005e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21270	positive	growth	50	thermophilic

Physiology and metabolism

Oxygen tolerance

21270

Oxygen toleranceanaerobe

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	denitrification	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	ribulose monophosphate pathway	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	gluconeogenesis	100	8 of 8
66794	kanosamine biosynthesis II	100	2 of 2
66794	biotin biosynthesis	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	suberin monomers biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	palmitate biosynthesis	100	22 of 22
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	vitamin B1 metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	photosynthesis	85.71	12 of 14
66794	glycolate and glyoxylate degradation	83.33	5 of 6
66794	vitamin B12 metabolism	82.35	28 of 34
66794	propionate fermentation	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	starch degradation	80	8 of 10
66794	lipoate biosynthesis	80	4 of 5
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	glycogen metabolism	80	4 of 5
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	isoleucine metabolism	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	proline metabolism	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	glutamate and glutamine metabolism	71.43	20 of 28
66794	heme metabolism	71.43	10 of 14
66794	pyrimidine metabolism	71.11	32 of 45
66794	glycolysis	70.59	12 of 17
66794	Entner Doudoroff pathway	70	7 of 10
66794	serine metabolism	66.67	6 of 9
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	formaldehyde oxidation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	1,4-dihydroxy-6-naphthoate biosynthesis	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	cyanate degradation	66.67	2 of 3
66794	tetrahydrofolate metabolism	64.29	9 of 14
66794	citric acid cycle	64.29	9 of 14
66794	purine metabolism	63.83	60 of 94
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	alanine metabolism	62.07	18 of 29
66794	phenylalanine metabolism	61.54	8 of 13
66794	methionine metabolism	61.54	16 of 26
66794	urea cycle	61.54	8 of 13
66794	methylglyoxal degradation	60	3 of 5
66794	cellulose degradation	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	coenzyme M biosynthesis	60	6 of 10
66794	oxidative phosphorylation	58.24	53 of 91
66794	non-pathway related	57.89	22 of 38
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	propanol degradation	57.14	4 of 7
66794	d-mannose degradation	55.56	5 of 9
66794	lipid metabolism	54.84	17 of 31
66794	arginine metabolism	54.17	13 of 24
66794	leucine metabolism	53.85	7 of 13
66794	histidine metabolism	51.72	15 of 29
66794	sphingosine metabolism	50	3 of 6
66794	cysteine metabolism	50	9 of 18
66794	ethanol fermentation	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	acetate fermentation	50	2 of 4
66794	tryptophan metabolism	50	19 of 38
66794	glycine metabolism	50	5 of 10
66794	lysine metabolism	45.24	19 of 42
66794	nitrate assimilation	44.44	4 of 9
66794	tyrosine metabolism	42.86	6 of 14
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	hydrogen production	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	glutathione metabolism	35.71	5 of 14
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	degradation of hexoses	33.33	6 of 18
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	degradation of sugar alcohols	31.25	5 of 16
66794	sulfate reduction	30.77	4 of 13
66794	polyamine pathway	30.43	7 of 23
66794	myo-inositol biosynthesis	30	3 of 10
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	degradation of sugar acids	28	7 of 25
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	ascorbate metabolism	27.27	6 of 22
66794	vitamin B6 metabolism	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	methanogenesis from CO2	25	3 of 12
66794	degradation of pentoses	25	7 of 28
66794	toluene degradation	25	1 of 4
66794	lipid A biosynthesis	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Engineered product
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
21270	water from the observation well of an underground gas storage	North Caucasus Region, Severo-Stavropolskoye	Russia	RUS	Europe

Taxonmaps

69479

Global distribution of 16S sequence KC242245 (>99% sequence identity) for Tepidibacillus fermentans subclade from Microbeatlas

Aquatic Samples	103
Soil Samples	71
Animal Samples	24
Plant Samples	48
Total Samples	246

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21270	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM434288v1 assembly for Tepidibacillus fermentans DSM 23802	scaffold	GCA_004342885	1281767.3	2784746787	1281767	62.26

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21270	Tepidibacillus fermentans strain STGH 16S ribosomal RNA gene, partial sequence	KC242245	1589		1281767

GC content

@ref	GC-content (mol%)	Method
21270	34.8	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Tepidibacillus fermentans DSM 23802
NCBI: GCA_004342885

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	77.30	no
125439	motility	BacteriaNetⓘ	yes	79.70	no
125439	gram_stain	BacteriaNetⓘ	variable	77.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	79.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Tepidibacillus fermentans DSM 23802
NCBI: GCA_004342885.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	72.00	no
125438	anaerobic	anaerobicⓘ	yes	74.60	yes
125438	aerobic	aerobicⓘ	no	82.22	yes
125438	spore-forming	spore-formingⓘ	yes	87.73	no
125438	thermophilic	thermophileⓘ	yes	55.34	no
125438	flagellated	motile2+ⓘ	yes	76.61	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tepidibacillus marianensis sp. nov., a novel heterotrophic iron-reducing bacterium isolated from Mariana Trench sediment.	Li S, Li C, Pei J, Liu R, Fang J, Wei Y, He Y, Li S, Feng Q, Zhang C, Guo T, Jiang Y, Hu Y, Jiang Z, Shi L, Dong Y.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006438	2024
Metabolism	Tepidibacillus fermentans gen. nov., sp. nov.: a moderately thermophilic anaerobic and microaerophilic bacterium from an underground gas storage.	Slobodkina GB, Panteleeva AN, Kostrikina NA, Kopitsyn DS, Bonch-Osmolovskaya EA, Slobodkin AI	Extremophiles	10.1007/s00792-013-0564-2	2013

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21270	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23802
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Tepidibacillus fermentans (DSM 23802, VKM B-2671, STGH)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004342885

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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