Name: Cellulomonas phragmiteti KB23
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2374

Type strain:

Species: Cellulomonas phragmiteti

Strain Designation: KB23

Culture col. no.: DSM 22512, JCM 19545, NBRC 110785, NCAIM B002303, NCAIM B.002303

Strain history: DSM 22512 <-- A. K. Borsodi KB23 <-- A. Rusznyák.

NCBI tax ID(s): 478780 (species)

SI-ID 400944

DSM 22512

Section

Cellulomonas phragmiteti KB23 is a mesophilic bacterium that was isolated from reed periphyton in a shallow soda pond.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Cellulomonadaceae*
	Genus	*Cellulomonas*
	Species	**Cellulomonas phragmiteti**
	Full Scientific Name (LPSN)	Cellulomonas phragmiteti Rusznyák et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %93.238

[Ref.: #69225]	Colony color	Dahlia yellow (1033)
[Ref.: #69225]	Cultivation medium used	ISP 3

[Ref.: #69225]	Colony color	Dahlia yellow (1033)
[Ref.: #69225]	Cultivation medium used	ISP 4

[Ref.: #69225]	Colony color	Daffodil yellow (1007)
[Ref.: #69225]	Cultivation medium used	ISP 5

[Ref.: #69225]	Colony color	Melon yellow (1028)
[Ref.: #69225]	Cultivation medium used	suter with tyrosine

[Ref.: #69225]	Colony color	Saffron yellow (1017)
[Ref.: #69225]	Cultivation medium used	ISP 7

[Ref.: #69225]	Colony color	Zinc yellow (1018)
[Ref.: #69225]	Cultivation medium used	suter without tyrosine

[Ref.: #69225]	Pigment production	no
[Ref.: #69225]	Pigment name	Melanin
[Ref.: #69225]	Pigment production	no
[Ref.: #69225]	Pigment name	soluble pigment

[Ref.: #69225]	Multicellular complex forming ability	no
[Ref.: #69225]	Multicellular complex name	Aerial mycelium
[Ref.: #69225]	Medium Name (multicellularity)	ISP 2

[Ref.: #69225]	Multicellular complex forming ability	no
[Ref.: #69225]	Multicellular complex name	Aerial mycelium
[Ref.: #69225]	Medium Name (multicellularity)	ISP 3

[Ref.: #69225]	Multicellular complex forming ability	no
[Ref.: #69225]	Multicellular complex name	Aerial mycelium
[Ref.: #69225]	Medium Name (multicellularity)	ISP 4

[Ref.: #69225]	Multicellular complex forming ability	no
[Ref.: #69225]	Multicellular complex name	Aerial mycelium
[Ref.: #69225]	Medium Name (multicellularity)	ISP 5

[Ref.: #69225]	Multicellular complex forming ability	no
[Ref.: #69225]	Multicellular complex name	Aerial mycelium
[Ref.: #69225]	Medium Name (multicellularity)	ISP 6

[Ref.: #69225]	Multicellular complex forming ability	no
[Ref.: #69225]	Multicellular complex name	Aerial mycelium
[Ref.: #69225]	Medium Name (multicellularity)	ISP 7

[Ref.: #69225]	Multicellular complex forming ability	no
[Ref.: #69225]	Multicellular complex name	Aerial mycelium
[Ref.: #69225]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69225]	Multicellular complex forming ability	no
[Ref.: #69225]	Multicellular complex name	Aerial mycelium
[Ref.: #69225]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69225]

Caption:

Plates (GYM, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69225]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69225]

Caption:

Plates (GYM, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69225]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16374]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #16374]

Culture medium growth

[Ref.: #16374]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16374]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16374]			growth	28 °C
[Ref.: #67770]			growth	28 °C

[Ref.: #16374]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.375

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69225]		NaCl		growth	0-5	%

[Ref.: #16374]	Murein short key	A21.04
[Ref.: #16374]	Murein types	A4ß L-Orn-D-Asp

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69225]	arabinose ChEBI	+	growth
[Ref.: #69225]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69225]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69225]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	+	fermentation	* from API Coryne
[Ref.: #69225]	inositol ChEBI	-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne
[Ref.: #69225]	mannose ChEBI	+	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69225]	raffinose ChEBI	+	growth
[Ref.: #69225]	rhamnose ChEBI	-	growth
[Ref.: #69225]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69225]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API Coryne
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69225]	3719	-	-	-	-	-	+	+	+	+	-	-	not determinedn.d.	+	-	+	+	+	-	+	+	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69225]	5903	not determinedn.d.	-	+/-	+/-	-	+	+/-	+/-	-	+/-	-	+/-	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16374]	Sample type/isolated from	reed (Phragmites australis) periphyton in a shallow soda pond
[Ref.: #16374]	Host species	Phragmites australis
[Ref.: #16374]	Geographic location (country and/or sea, region)	Budapest, Kiskunság National Park
[Ref.: #16374]	Country	Hungary
[Ref.: #16374]	Country ISO 3 Code	HUN
[Ref.: #16374]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Phragmites australis-associated biofilm originating from a soda pond in Kiskunság National Park
[Ref.: #67770]	Host species	Phragmites australis
[Ref.: #67770]	Country	Hungary
[Ref.: #67770]	Country ISO 3 Code	HUN
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Pond (small)
#Host	#Other	-
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Condition	#Alkaline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16374]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16374]	Cellulomonas sp. KB23 partial 16S rRNA gene, strain KB23	AM902253	453	ENA	478780 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cellulomonas phragmiteti NBRC 110785	GCA_016862775	contig	GenBank	478780 tax ID	*
[Ref.: #66792]	Cellulomonas phragmiteti strain NBRC 110785	478780.3	wgs	PATRIC	478780 tax ID	*

[Ref.: #16374]

GC-content

74.8 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cellulomonas phragmiteti strain NBRC 110785 PATRIC: 478780.3
[Ref.: #69481]	spore-forming	yes	73	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cellulomonas phragmiteti strain NBRC 110785 PATRIC: 478780.3
[Ref.: #69480]	gram-positive	yes	91.806	no
[Ref.: #69480]	anaerobic	no	98.926	no
[Ref.: #69480]	halophile	no	83.347	no
[Ref.: #69480]	spore-forming	no	84.88	no
[Ref.: #69480]	glucose-util	yes	90.604	no
[Ref.: #69480]	aerobic	yes	89.703	no
[Ref.: #69480]	thermophile	no	99.756	yes
[Ref.: #69480]	flagellated	no	97.019	no
[Ref.: #69480]	motile	no	87.033	no
[Ref.: #69480]	glucose-ferment	no	74.009	no

Availability in culture collections External links

[Ref.: #16374]

Culture collection no.

DSM 22512, JCM 19545, NBRC 110785, NCAIM B002303, NCAIM B.002303

[Ref.: #71929]

SI-ID 400944

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond.	Rusznyak A, Toth EM, Schumann P, Sproer C, Makk J, Szabo G, Vladar P, Marialigeti K, Borsodi AK	Int J Syst Evol Microbiol	10.1099/ijs.0.022608-0	2010	*

References

#16374

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22512

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69225

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71929

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400944.1 )

* These data were automatically processed and therefore are not curated

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Cellulomonas phragmiteti strain NBRC 110785

Cellulomonas phragmiteti strain NBRC 110785

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