Oceanobacillus locisalsi (DSM 27649, CCUG 56608, CHL-21)

Name: Oceanobacillus locisalsi (DSM 27649, CCUG 56608, CHL-21)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 23736

Type strain

Strain Designation CHL-21

Culture col. no. DSM 27649 CCUG 56608 CHL-21 KCTC 13253 CIP 110275

NCBI tax ID(s) 546107

Special Collections DSMZ CCUG KCTC Literature strains CIP

Links

Oceanobacillus locisalsi CHL-21 is an anaerobe, spore-forming, mesophilic prokaryote that was isolated from sediment sample, marine solar saltern.

spore-forming Gram-variable motile rod-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 374240

CCUG 56608,KCTC 13253,DSM 27649,CIP 110275

@ref 20215

Last LPSN update 2025-12-16 09:52:43

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Oceanobacillus

Species Oceanobacillus locisalsi

Full scientific name Oceanobacillus locisalsi Lee et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Flagellum arrangement
29826	variable	1.3-4 µm	0.6-1 µm	rod-shaped
67771				rod-shaped	monotrichous, polar
67771	variable
120640	positive			ovoid-shaped

Colony morphology

61040

Incubation period1 day

Pigmentation

29826

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
20696	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 50.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40054	Marine agar (MA)		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)
120640	CIP Medium 13	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
20696	positive	growth	30	mesophilic
29826	positive	growth	10-45
29826	positive	optimum	30-37	mesophilic
40054	positive	growth	30	mesophilic
61040	positive	growth	37	mesophilic
67771	positive	growth	30-37	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
29826	positive	optimum	7-7.5
29826	positive	growth	06-09	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
29826	anaerobe
61040	aerobe
67771	aerobe

Spore formation

29826

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29826	NaCl	positive	growth	0-25 %
29826	NaCl	positive	optimum	05-10 %

Observation

@ref	Observation
29826	aggregates in chains
67771	quinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29826	22599 ChEBI	arabinose	+	carbon source
29826	4853 ChEBI	esculin	+	hydrolysis
29826	28757 ChEBI	fructose	+	carbon source
29826	29864 ChEBI	mannitol	+	carbon source
29826	17632 ChEBI	nitrate	+	reduction
120640	17632 ChEBI	nitrate	+	reduction
120640	16301 ChEBI	nitrite	-	reduction
29826	30911 ChEBI	sorbitol	+	carbon source
29826	17992 ChEBI	sucrose	+	carbon source
29826	27082 ChEBI	trehalose	+	carbon source
29826	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120640	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
29826	catalase	+	1.11.1.6
120640	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120640	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
120640	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120640	-	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Sampling date
20696	sediment sample, marine solar saltern	Yellow Sea	Republic of Korea	KOR	Asia	35.6997	126.576 35.6997/126.576
61040	Soil,marine solar saltern	Yellow Sea	Republic of Korea	KOR	Asia			2007-06-01
67771	From marine solar saltern of the Yellow Sea		Republic of Korea	KOR	Asia
120640	Environment, Marine solar saltern	Yellow sea	Republic of Korea	KOR	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
20696	1	Risk group (German classification)
120640	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4267598v1 assembly for Oceanobacillus locisalsi CCUG 56608	scaffold	GCA_042675985	546107	23.72

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20696	Oceanobacillus locisalsi strain CHL-21 16S ribosomal RNA gene, partial sequence	EU817570	1520		546107

GC content

20696

GC-content (mol%)39.8

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanobacillus aidingensis sp. nov., a moderately halophilic bacterium.	Liu W, Yang SS	Antonie Van Leeuwenhoek	10.1007/s10482-014-0128-1	2014
Phylogeny	Oceanobacillus locisalsi sp. nov., isolated from a marine solar saltern.	Lee SY, Oh TK, Kim W, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijs.0.021907-0	2010

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20696	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27649
#29826	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26202 (see below)
#40054	; Curators of the CIP;
#61040	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56608
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68382	Automatically annotated from API zym .
#120640	Collection of Institut Pasteur ; Curators of the CIP; CIP 110275
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Oceanobacillus locisalsi (DSM 27649, CCUG 56608, CHL-21)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042675985

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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